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PurposeEXPRESs plasmid for human erythrocyte encoding BSG/EMMPRIN/CD147 and CD4d3+4-Comp-His-Stop pentamer
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36147 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBasigin
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Alt nameBSG
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SpeciesH. sapiens (human)
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Insert Size (bp)970
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Entrez GeneBSG (a.k.a. 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, TCSF)
- Promoter CMV
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Tags
/ Fusion Proteins
- ratCD4d3+4 (C terminal on insert)
- pentamerization domain of rat cartilaginous oligomeric matrix protein (COMP) (C terminal on insert)
- His tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BasiginCD4d3+4-Comp-His-Stop was a gift from Gavin Wright (Addgene plasmid # 36147 ; http://n2t.net/addgene:36147 ; RRID:Addgene_36147) -
For your References section:
Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner JC, Wright GJ. Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606. 10.1038/nature10606 PubMed 22080952