Addgene YopH (residues 164-468) Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: YopH (residues 164-468) Sequencing Result


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PspOMI (34) HincII - HpaI (4298) BsmBI - Esp3I (4185) BstBI (3740) SgrAI (3143) NspI (3120) PciI (3116) BmtI (3020) NheI (3016) BspHI (2969) BfuAI - BspMI (2571) BglI (2293) ApaI - BanII (38) LacI-R (558 .. 577) BstAPI (565) AclI (581) PfoI (673) EcoNI (709) T7 (720 .. 739) T7 promoter PacI (779) NotI (784) PstI - SbfI (796) AvaI - BsoBI - PaeR7I - PspXI - XhoI (800) XbaI (807) RBS BsaBI * (844) MscI (875) BsaI (1333) AccI (1357) BstZ17I (1358) MfeI (1419) PshAI (1438) TatI (1674) BamHI (1793) AsiSI (1803) AscI (1808) CsiI - SexAI * (1815) DraIII (1819) FseI (1827) T7 Term (1898 .. 1916) EcoO109I (1928) Bsu36I (1967) AgeI (2016) DrdI - PflFI - Tth111I (2076) BsaAI (2165) YopH (residues 164-468) 4559 bp

Sequence View

PspOMIBanIIApaI 5ʹ 3ʹ 60 195 190 185 180 ALRLRASVSSLPGALLAIQQ lacI LRLRASVSSLPGALLAIQQ g c c a g a c g c a g a c g c g c c g a g a c a g a a c t t a a t g g g c c c g c t a a c a g c g c g a t t t g c t g g c g g t c t g c g t c t g c g c g g c t c t g t c t t g a a t t a c c c g g g c g a t t g t c g c g c t a a a c g a c c

120 175 170 165 160 HGLAVLHEVGLRTGDEHSFI lacI HGLAVLHEVGLRTGDEHSFI t g a c c c a a t g c g a c c a g a t g c t c c a c g c c c a g t c g c g t a c c g t c t t c a t g g g a g a a a a t a a c t g g g t t a c g c t g g t c t a c g a g g t g c g g g t c a g c g c a t g g c a g a a g t a c c c t c t t t t a t

180 155 150 145 140 ISNIPTQDSVDLFLAPVNTC lacI ISNIPTQDSVDLFLAPVNTC a t a c t g t t g a t g g g t g t c t g g t c a g a g a c a t c a a g a a a t a a c g c c g g a a c a t t a g t g c a g t a t g a c a a c t a c c c a c a g a c c a g t c t c t g t a g t t c t t t a t t g c g g c c t t g t a a t c a c g t c

240 135 130 125 120 AAEVAIADQDDLPYNIILGS lacI AAEVAIADQDDLPYNIILGS g c a g c t t c c a c a g c a a t g g c a t c c t g g t c a t c c a g c g g a t a g t t a a t g a t c a g c c c a c t g c g t c g a a g g t g t c g t t a c c g t a g g a c c a g t a g g t c g c c t a t c a a t t a c t a g t c g g g t g a c

300 115 110 105 100 VRQALLNHVAAKCAEVGSRE lacI VRQALLNHVAAKCAEVGSRE a c g c g t t g c g c g a g a a g a t t g t g c a c c g c c g c t t t a c a g g c t t c g a c g c c g c t t c g t t c t t g c g c a a c g c g c t c t t c t a a c a c g t g g c g g c g a a a t g t c c g a a g c t g c g g c g a a g c a a g a

360 95 90 85 80 VMSVVVSAGLQDARSKIAAV lacI VMSVVVSAGLQDARSKIAAV a c c a t c g a c a c c a c c a c g c t g g c a c c c a g t t g a t c g g c g c g a g a t t t a a t c g c c g c g a c a t g g t a g c t g t g g t g g t g c g a c c g t g g g t c a a c t a g c c g c g c t c t a a a t t a g c g g c g c t g t

420 75 70 65 60 IQSPAHLALSSTAVGILLSQ lacI IQSPAHLALSSTAVGILLSQ a t t t g c g a c g g c g c g t g c a g g g c c a g a c t g g a g g t g g c a a c g c c a a t c a g c a a c g a c t g t t a a a c g c t g c c g c g c a c g t c c c g g t c t g a c c t c c a c c g t t g c g g t t a g t c g t t g c t g a c a

480 55 50 45 40 KGALQQAVRNPIYNLEAMAA lacI KGALQQAVRNPIYNLEAM t t g c c c g c c a g t t g t t g t g c c a c g c g g t t g g g a a t g t a a t t c a g c t c c g c c a t c g c c g c t a a c g g g c g g t c a a c a a c a c g g t g c g c c a a c c c t t a c a t t a a g t c g a g g c g g t a g c g g c g a

540 35 30 25 20 EVKERTKASVHSAQNVVRSV lacI t c c a c t t t t t c c c g c g t t t t c g c a g a a a c g t g g c t g g c c t g g t t c a c c a c g c g g g a a a c g a g g t g a a a a a g g g c g c a a a a g c g t c t t t g c a c c g a c c g g a c c a a g t g g t g c g c c c t t t g c

AclIBstAPI 600 15 10 5 1 TQYSVGAYEAVDYLTVPKM lacI lacI promoter G G C A T A C T C T G C G A C A T C G T LacI-R g t c t g a t a a g a g a c a c c g g c a t a c t c t g c g a c a t c g t a t a a c g t t a c t g g t t t c a c a t t c c a g a c t a t t c t c t g t g g c c g t a t g a g a c g c t g t a g c a t a t t g c a a t g a c c a a a g t g t a a g

660 lacI promoter a c c a c c c t g a a t t g a c t c t c t t c c g g g c g c t a t c a t g c c a t a c c g c g a a a g g t t t t g c g c t g g t g g g a c t t a a c t g a g a g a a g g c c c g c g a t a g t a c g g t a t g g c g c t t t c c a a a a c g c g

EcoNIPfoI 720 lacI promoter T7 promoter T T7 c a t t c g a t g g t g t c c g g g a t c t c g a c g c t c t c c c t t a t g c g a c t c c t g c a t t a g g a a a t t g t a a g c t a c c a c a g g c c c t a g a g c t g c g a g a g g g a a t a c g c t g a g g a c g t a a t c c t t t a a

PacI 780 T7 promoter lac operator A A T A C G A C T C A C T A T A G G G T7 a a t a c g a c t c a c t a t a g g g a g a g a a t t g t g a g c g g a t a a c a a t t c c c c t g t a g a t t a a t t t t a t g c t g a g t g a t a t c c c t c t c t t a a c a c t c g c c t a t t g t t a a g g g g a c a t c t a a t t a a

XbaIPaeR7IBsoBIPspXIXhoIAvaISbfIPstINotI 840 RBS a a g c g g c c g c c c t g c a g g a c t c g a g t t c t a g a a a t a a t t t t g t t t a a c t t t a a g a a g g a g t t c g c c g g c g g g a c g t c c t g a g c t c a a g a t c t t t a t t a a a a c a a a t t g a a a t t c t t c c t c

MscIBsaBI* 900 RBS MRERPHTSGHHGAGEAR a t a t a g a t c a t g c g t g a a c g a c c a c a c a c t t c t g g c c a t c a c g g g g c t g g c g a a g c c a g a t a t a t c t a g t a c g c a c t t g c t g g t g t g t g a a g a c c g g t a g t g c c c c g a c c g c t t c g g t c t

960 ATAPSTVSPYGPEARAELSS g c c a c c g c a c c a a g c a c t g t t t c t c c t t a t g g c c c a g a a g c g c g c g c a g a a c t c a g c a g c c g g t g g c g t g g t t c g t g a c a a a g a g g a a t a c c g g g t c t t c g c g c g c g t c t t g a g t c g t c g

1020 RLTTLRNTLAPATNDPRYLQ c g c c t c a c c a c a t t g c g c a a t a c g c t g g c g c c a g c a a c g a a t g a t c c g c g t t a c t t a c a a g c g g a g t g g t g t a a c g c g t t a t g c g a c c g c g g t c g t t g c t t a c t a g g c g c a a t g a a t g t t

1080 ACGGEKLNRFRDIQCCRQTA g c c t g c g g c g g t g a a a a g c t a a a c c g a t t t a g a g a t a t t c a a t g c t g t c g g c a a a c c g c a c g g a c g c c g c c a c t t t t c g a t t t g g c t a a a t c t c t a t a a g t t a c g a c a g c c g t t t g g c g t

1140 VRADLNANYIQVGNTRTIAC g t a c g c g c c g a t c t t a a t g c c a a t t a c a t c c a g g t c g g t a a c a c t c g t a c c a t a g c g t g c c a t g c g c g g c t a g a a t t a c g g t t a a t g t a g g t c c a g c c a t t g t g a g c a t g g t a t c g c a c g

1200 QYPLQSQLESHFRMLAENRT c a g t a t c c g c t a c a a t c t c a a c t t g a a a g c c a t t t c c g t a t g c t g g c a g a a a a c c g a a c g g t c a t a g g c g a t g t t a g a g t t g a a c t t t c g g t a a a g g c a t a c g a c c g t c t t t t g g c t t g c

1260 PVLAVLASSSEIANQRFGMP c c a g t g t t g g c t g t t t t a g c g t c c a g t t c t g a g a t a g c c a a t c a a a g a t t c g g t a t g c c a g g t c a c a a c c g a c a a a a t c g c a g g t c a a g a c t c t a t c g g t t a g t t t c t a a g c c a t a c g g t

1320 DYFRQSGTYGSITVESKMTQ g a t t a t t t c c g c c a g a g t g g t a c c t a t g g c a g t a t c a c t g t a g a g t c t a a a a t g a c t c a g c t a a t a a a g g c g g t c t c a c c a t g g a t a c c g t c a t a g t g a c a t c t c a g a t t t t a c t g a g t c

AccIBstZ17IBsaI 1380 QVGLGDGIMADMYTLTIREA c a a g t t g g t c t c g g t g a c g g g a t t a t g g c a g a t a t g t a t a c t t t a a c g a t t c g t g a a g c g g t t c a a c c a g a g c c a c t g c c c t a a t a c c g t c t a t a c a t a t g a a a t t g c t a a g c a c t t c g c

PshAIMfeI 1440 GQKTISVPVVHVGNWPDQTA g g t c a a a a a a c a a t c t c t g t t c c t g t g g t t c a t g t t g g c a a t t g g c c c g a t c a g a c c g c a c c a g t t t t t t g t t a g a g a c a a g g a c a c c a a g t a c a a c c g t t a a c c g g g c t a g t c t g g c g t

1500 VSSEVTKALASLVDQTAETK g t c a g c t c t g a a g t t a c c a a g g c a c t c g c t t c a c t g g t a g a t c a a a c a g c a g a a a c a a a a c a g t c g a g a c t t c a a t g g t t c c g t g a g c g a a g t g a c c a t c t a g t t t g t c g t c t t t g t t t t

1560 RNMYESKGSSAVGDDSKLRP c g c a a t a t g t a t g a a a g c a a a g g a a g t t c a g c g g t a g g a g a t g a c t c c a a a t t a c g g c c g g c g t t a t a c a t a c t t t c g t t t c c t t c a a g t c g c c a t c c t c t a c t g a g g t t t a a t g c c g g c

1620 VIHCRAGVGRTAQLIGAMCM g t a a t a c a t t g c c g t g c g g g t g t t g g c c g t a c t g c g c a a c t g a t t g g c g c a a t g t g c a t g c a t t a t g t a a c g g c a c g c c c a c a a c c g g c a t g a c g c g t t g a c t a a c c g c g t t a c a c g t a c

TatI 1680 NDSRNSQLSVEDMVSQMRVQ a a t g a t a g t c g t a a t a g t c a g t t a a g c g t a g a a g a t a t g g t c a g c c a a a t g c g a g t a c a a t t a c t a t c a g c a t t a t c a g t c a a t t c g c a t c t t c t a t a c c a g t c g g t t t a c g c t c a t g t t

1740 RNGIMVQKDEQLDVLIKLAE a g a a a t g g t a t t a t g g t a c a a a a a g a t g a g c a a c t t g a t g t t c t g a t t a a g t t g g c t g a a t c t t t a c c a t a a t a c c a t g t t t t t c t a c t c g t t g a a c t a c a a g a c t a a t t c a a c c g a c t t

BamHI 1800 GQGRPLLNS* g g a c a a g g g c g a c c a t t a t t a a a t a g c t a a t a a t a a g a t c c c a a c t c c a t a a g g a t c c g c c c t g t t c c c g c t g g t a a t a a t t t a t c g a t t a t t a t t c t a g g g t t g a g g t a t t c c t a g g c g

FseIAsiSIAscISexAI*CsiIDraIII 1860 g a t c g c g g c g c g c c a c c t g g t g g c c g g c c g g t a c c a c g c g t g c g c g c t g a t c c g g c t g c t c t a g c g c c g c g c g g t g g a c c a c c g g c c g g c c a t g g t g c g c a c g c g c g a c t a g g c c g a c g a

1920 T7 terminator G G C G A C T C G T T A T T G A T C G T7 Term a a c a a a g c c c g a a a g g a a g c t g a g t t g g c t g c t g c c a c c g c t g a g c a a t a a c t a g c a t a a t t g t t t c g g g c t t t c c t t c g a c t c a a c c g a c g a c g g t g g c g a c t c g t t a t t g a t c g t a t t

Bsu36IEcoO109I 1980 T7 terminator c c c c t t g g g g c c t c t a a a c g g g t c t t g a g g g g t t t t t t g c t g a a a c c t c a g g c a t t t g a g g g g g a a c c c c g g a g a t t t g c c c a g a a c t c c c c a a a a a a c g a c t t t g g a g t c c g t a a a c t c

AgeI 2040 a a g c a c a c g g t c a c a c t g c t t c c g g t a g t c a a t a a a c c g g t a a a c c a g c a a t a g a c a t a a t t c g t g t g c c a g t g t g a c g a a g g c c a t c a g t t a t t t g g c c a t t t g g t c g t t a t c t g t a t t

Tth111IPflFIDrdI 2100 g c g g c t a t t t a a c g a c c c t g c c c t g a a c c g a c g a c c g g g t c a t c g t g g c c g g a t c t t g c g c g c c g a t a a a t t g c t g g g a c g g g a c t t g g c t g c t g g c c c a g t a g c a c c g g c c t a g a a c g c

2160 260 255 *KGVVKTIEG SmR *KGVVKTIEG g c c c c t c g g c t t g a a c g a a t t g t t a g a c a t t a t t t g c c g a c t a c c t t g g t g a t c t c g c c t c g g g g a g c c g a a c t t g c t t a a c a a t c t g t a a t a a a c g g c t g a t g g a a c c a c t a g a g c g g a

BsaAI 2220 250 245 240 235 KVYHVFEELQDARSALRDEE SmR KVYHVFEELQDARSALRDEE t t c a c g t a a t g g a c a a a t t c t t c c a a c t g a t c t g c g c g c g a g g c c a a g c g a t c t t c t t c t a a g t g c a t t a c c t g t t t a a g a a g g t t g a c t a g a c g c g c g c t c c g g t t c g c t a g a a g a a g a

2280 230 225 220 215 QGLYAQRAELIVPQYQAPLR SmR QGLYAQRAELIVPQYQAPLR t g t c c a a g a t a a g c c t g t c t a g c t t c a a g t a t g a c g g g c t g a t a c t g g g c c g g c a g g c g c a c a g g t t c t a t t c g g a c a g a t c g a a g t t c a t a c t g c c c g a c t a t g a c c c g g c c g t c c g c g

BglI 2340 210 205 200 195 EMAWDAAVDKPAIKGTVASY SmR EMAWDAAVDKPAIKGTVASY t c c a t t g c c c a g t c g g c a g c g a c a t c c t t c g g c g c g a t t t t g c c g g t t a c t g c g c t g t a c a g g t a a c g g g t c a g c c g t c g c t g t a g g a a g c c g c g c t a a a a c g g c c a a t g a c g c g a c a t g

2400 190 185 180 175 WIRSLTLVVNREDGAWDPPS SmR WIRSLTLVVNREDGAWDPPS c a a a t g c g g g a c a a c g t a a g c a c t a c a t t t c g c t c a t c g c c a g c c c a g t c g g g c g g c g a g g t t t a c g c c c t g t t g c a t t c g t g a t g t a a a g c g a g t a g c g g t c g g g t c a g c c c g c c g c t c

2460 170 165 160 155 NWLTLTENLAEFLDQEPVPD SmR NWLTLTENLAEFLDQEPVPD t t c c a t a g c g t t a a g g t t t c a t t t a g c g c c t c a a a t a g a t c c t g t t c a g g a a c c g g a t c a a a g g t a t c g c a a t t c c a a a g t a a a t c g c g g a g t t t a t c t a g g a c a a g t c c t t g g c c t a g t

2520 150 145 140 135 FLEEAAPGVLAVSHERAKTL SmR FLEEAAPGVLAVSHERAKTL a a g a g t t c c t c c g c c g c t g g a c c t a c c a a g g c a a c g c t a t g t t c t c t t g c t t t t g t c a g c t t c t c a a g g a g g c g g c g a c c t g g a t g g t t c c g t t g c g a t a c a a g a g a a c g a a a a c a g t c g

BspMIBfuAI 2580 130 125 120 115 LIALDIDITAPEFIGALIDN SmR LIALDIDITAPEFIGALIDN a a g a t a g c c a g a t c a a t g t c g a t c g t g g c t g g c t c g a a g a t a c c t g c a a g a a t g t c a t t g t t c t a t c g g t c t a g t t a c a g c t a g c a c c g a c c g a g c t t c t a t g g a c g t t c t t a c a g t a a c

2640 110 105 100 95 RQWEGFQLERKAPYRWPIID SmR RQWEGFQLERKAPYRWPIID c g c t g c c a t t c t c c a a a t t g c a g t t c g c g c t t a g c t g g a t a a c g c c a c g g a a t g a t g t c g g c g a c g g t a a g a g g t t t a a c g t c a a g c g c g a a t c g a c c t a t t g c g g t g c c t t a c t a c a g c

2700 90 85 80 75 DHVVITVEVARLIESEGPSA SmR DHVVITVEVARLIESEGPSA t c g t g c a c a a c a a t g g t g a c t t c t a c a g c g c g g a g a a t c t c g c t c t c t c c a g g g g a a g c c a g c a c g t g t t g t t a c c a c t g a a g a t g t c g c g c c t c t t a g a g c g a g a g a g g t c c c c t t c g g

2760 70 65 60 55 STELLDNILARRTTEDLRVT SmR STELLDNILARRTTEDLRVT g a a g t t t c c a a a a g g t c g t t g a t c a a a g c t c g c c g c g t t g t t t c a t c a a g c c t t a c g g t c c t t c a a a g g t t t t c c a g c a a c t a g t t t c g a g c g g c g c a a c a a a g t a g t t c g g a a t g c c a g

2820 50 45 40 35 VTVLLDIDSHPKLGGDVASG SmR VTVLLDIDSHPKLGGDVASG a c c g t a a c c a g c a a a t c a a t a t c a c t g t g c g g c t t c a g g c c g c c a t c c a c t g c g g a g c c g t g g c a t t g g t c g t t t a g t t a t a g t g a c a c g c c g a a g t c c g g c g g t a g g t g a c g c c t c g g c

2880 30 25 20 15 YLHVALLTPELHREIVGVVE SmR YLHVALLTPELHREIVGVVE t a c a a a t g t a c g g c c a g c a a c g t c g g t t c g a g a t g g c g c t c g a t g a c g c c a a c t a c c t c t a t g t t t a c a t g c c g g t c g t t g c a g c c a a g c t c t a c c g c g a g c t a c t g c g g t t g a t g g a g a

2940 10 5 1 SLQTSVEAIVAERM SmR AmpR promoter SLQTSVEAIVAERM g a t a g t t g a g t c g a t a c t t c g g c g a t c a c c g c t t c c c t c a t a c t c t t c c t t t t t c a t t a t c t a t c a a c t c a g c t a t g a a g c c g c t a g t g g c g a a g g g a g t a t g a g a a g g a a a a a g t a a t a

BspHI 3000 AmpR promoter t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c

BmtINheI 3060 AmpR promoter a a a a a t a a a c a a a t a g c t a g c t c a c t c g g t c g c t a c g c t c c g g g c g t g a g a c t g c g g c g g t t t t t a t t t g t t t a t c g a t c g a g t g a g c c a g c g a t g c g a g g c c c g c a c t c t g a c g c c g c c

NspIPciI 3120 g c g c t g c g g a c a c a t a c a a a g t t a c c c a c a g a t t c c g t g g a t a a g c a g g g g a c t a a c a t g c g c g a c g c c t g t g t a t g t t t c a a t g g g t g t c t a a g g c a c c t a t t c g t c c c c t g a t t g t a c

SgrAI 3180 t g a g g c a a a a c a g c a g g g c c g c g c c g g t g g c g t t t t t c c a t a g g c t c c g c c c t c c t g c c a a c t c c g t t t t g t c g t c c c g g c g c g g c c a c c g c a a a a a g g t a t c c g a g g c g g g a g g a c g g t

3240 g a g t t c a c a t a a a c a g a c g c t t t t c c g g t g c a t c t g t g g g a g c c g t g a g g c t c a a c c a t g c t c a a g t g t a t t t g t c t g c g a a a a g g c c a c g t a g a c a c c c t c g g c a c t c c g a g t t g g t a c

3300 a a t c t g a c a g t a c g g g c g a a a c c c g a c a g g a c t t a a a g a t c c c c a c c g t t t c c g g c g g g t t t a g a c t g t c a t g c c c g c t t t g g g c t g t c c t g a a t t t c t a g g g g t g g c a a a g g c c g c c c a

3360 c g c t c c c t c t t g c g c t c t c c t g t t c c g a c c c t g c c g t t t a c c g g a t a c c t g t t c c g c c t t g c g a g g g a g a a c g c g a g a g g a c a a g g c t g g g a c g g c a a a t g g c c t a t g g a c a a g g c g g a a

3420 t c t c c c t t a c g g g a a g t g t g g c g c t t t c t c a t a g c t c a c a c a c t g g t a t c t c g g c t c g g t a g a g g g a a t g c c c t t c a c a c c g c g a a a g a g t a t c g a g t g t g t g a c c a t a g a g c c g a g c c a

3480 g t a g g t c g t t c g c t c c a a g c t g g g c t g t a a g c a a g a a c t c c c c g t t c a g c c c g a c t g c t g c a t c c a g c a a g c g a g g t t c g a c c c g a c a t t c g t t c t t g a g g g g c a a g t c g g g c t g a c g a c

3540 c g c c t t a t c c g g t a a c t g t t c a c t t g a g t c c a a c c c g g a a a a g c a c g g t a a a a c g c c a c t g c g g a a t a g g c c a t t g a c a a g t g a a c t c a g g t t g g g c c t t t t c g t g c c a t t t t g c g g t g a

3600 g g c a g c a g c c a t t g g t a a c t g g g a g t t c g c a g a g g a t t t g t t t a g c t a a a c a c g c g g t t g c c g t c g t c g g t a a c c a t t g a c c c t c a a g c g t c t c c t a a a c a a a t c g a t t t g t g c g c c a a c

3660 c t c t t g a a g t g t g c g c c a a a g t c c g g c t a c a c t g g a a g g a c a g a t t t g g t t g c t g t g c t c g a g a a c t t c a c a c g c g g t t t c a g g c c g a t g t g a c c t t c c t g t c t a a a c c a a c g a c a c g a g

3720 t g c g a a a g c c a g t t a c c a c g g t t a a g c a g t t c c c c a a c t g a c t t a a c c t t c g a t c a a a c c a c g c t t t c g g t c a a t g g t g c c a a t t c g t c a a g g g g t t g a c t g a a t t g g a a g c t a g t t t g g

BstBI 3780 a c c t c c c c a g g t g g t t t t t t c g a a g g t t a a g t c a g t t g g g g a a c t g c t t a a c c t t c g a t c t g g a g g g g t c c a c c a a a a a a g c t t c c a a t t c a g t c a a c c c c t t g a c g a a t t g g a a g c t a g

3840 a a a c c a c c t c c c c a g g t g g t t t t t t c g t t t a c a g g g c a a a a g a t t a c g c g c a g a a a a a a a t t t g g t g g a g g g g t c c a c c a a a a a a g c a a a t g t c c c g t t t t c t a a t g c g c g t c t t t t t t t

3900 g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c t g a a c c g c t c t t a t t t c a g t g c a a t t t a c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g a c t t g g c g a g a a t a a a g t c a c g t t a a a t

3960 t c t c t t c a a a t g t a g c a c c t g a a g t c a g c c c c a t a c g a t a t a a g t t g t a a t t c t c a t g t t a g a g a a g t t t a c a t c g t g g a c t t c a g t c g g g g t a t g c t a t a t t c a a c a t t a a g a g t a c a a

4020 a g t c a t g c c c c g c g c c c a c c g g a a g g a g c t g a c t g g g t t g a a g g c t c t c a a g g g c a t c g g t c a g t a c g g g g c g c g g g t g g c c t t c c t c g a c t g a c c c a a c t t c c g a g a g t t c c c g t a g c c

4080 360 *QG lacI *QG t c g a g a t c c c g g t g c c t a a t g a g t g a g c t a a c t t a c a t t a a t t g c g t t g c g c t c a c t g c c a g c t c t a g g g c c a c g g a t t a c t c a c t c g a t t g a a t g t a a t t a a c g c a a c g c g a g t g a c g g

4140 355 350 345 340 SELRSVQRALQMLSDALARP lacI SELRSVQRALQMLSDALARP c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c

BsmBIEsp3I 4200 335 330 325 320 SATQTNPALTTKRKVLSVPL lacI SATQTNPALTTKRKVLSVPL g a g a g g c g g t t t g c g t a t t g g g c g c c a g g g t g g t t t t t c t t t t c a c c a g t g a g a c g g g c a c t c t c c g c c a a a c g c a t a a c c c g c g g t c c c a c c a a a a a g a a a a g t g g t c a c t c t g c c c g t

4260 315 310 305 300 LQNGKVAQGQSLQLLRDVST lacI LQNGKVAQGQSLQLLRDVST a c a g c t g a t t g c c c t t c a c c g c c t g g c c c t g a g a g a g t t g c a g c a a g c g g t c c a c g c t g g t g t c g a c t a a c g g g a a g t g g c g g a c c g g g a c t c t c t c a a c g t c g t t c g c c a g g t g c g a c c

HincIIHpaI 4320 295 290 285 280 QGLLRFDQKITTLPPIYCSS lacI QGLLRFDQKITTLPPIYCSS t t t g c c c c a g c a g g c g a a a a t c c t g t t t g a t g g t g g t t a a c g g c g g g a t a t a a c a t g a g c a a a c g g g g t c g t c c g c t t t t a g g a c a a a c t a c c a c c a a t t g c c g c c c t a t a t t g t a c t c g

4380 275 270 265 260 DETDDYGVVSIDAGVRLGSE lacI DETDDYGVVSIDAGVRLGSE t g t c t t c g g t a t c g t c g t a t c c c a c t a c c g a g a t g t c c g c a c c a a c g c g c a g c c c g g a c t a c a g a a g c c a t a g c a g c a t a g g g t g a t g g c t c t a c a g g c g t g g t t g c g c g t c g g g c c t g a

4440 255 250 245 240 TIARMAGLAMQDNAVLMATP lacI TIARMAGLAMQDNAVLMATP c g g t a a t g g c g c g c a t t g c g c c c a g c g c c a t c t g a t c g t t g g c a a c c a g c a t c g c a g t g g g c c a t t a c c g c g c g t a a c g c g g g t c g c g g t a g a c t a g c a a c c g t t g g t c g t a g c g t c a c c

4500 235 230 225 220 VIGENLMQMTQQFGSMASWD lacI VIGENLMQMTQQFGSMASWD g a a c g a t g c c c t c a t t c a g c a t t t g c a t g g t t t g t t g a a a a c c g g a c a t g g c a c t c c a g t c t t g c t a c g g g a g t a a g t c g t a a a c g t a c c a a a c a a c t t t t g g c c t g t a c c g t g a g g t c a

3ʹ 5ʹ 4559 215 210 205 200 GEREAIPQIQNRTLYKHWG lacI GEREAIPQIQNRTLYKHWG c g c c t t c c c g t t c c g c t a t c g g c t g a a t t t g a t t g c g a g t g a g a t a t t t a t g c c a g c c a g c g g a a g g g c a a g g c g a t a g c c g a c t t a a a c t a a c g c t c a c t c t a t a a a t a c g g t c g g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.