Addgene AAV-PB-SRT-tdTomato_BC18 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: AAV-PB-SRT-tdTomato_BC18 Sequencing Result


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PsiI (200) Amp-R (6199 .. 6218) BpmI (5719) pBR322ori-F (4897 .. 4916) KpnI (4327) Acc65I (4323) BlpI (4268) EagI - NotI (4193) NsiI (4063) MreI - SgrAI (3775) PmeI (3753) BsaI (3737) PspXI (3691) BspDI - ClaI (3681) BsrGI (3670) M13/pUC Forward (379 .. 401) M13 Forward (393 .. 410) M13 fwd T7 (421 .. 440) T7 promoter PpuMI (1018) XbaI (1035) MfeI (1127) BglII (1626) PspOMI (1673) ApaI (1677) AflIII - PciI (1693) FseI (1764) PasI (1808) BstAPI (1840) BbvCI - Bpu10I (1962) DraIII (2096) EF1a-F (2186 .. 2206) AAV-PB-SRT-tdTomato_BC18 6506 bp

Sequence View

60 g c t a a t a a c a g g c c t g c t g g t a a t c g c a g g c c t t t t t a t t t g g t g t a t t t a g a a a a a t a a c g a t t a t t g t c c g g a c g a c c a t t a g c g t c c g g a a a a a t a a a c c a c a t a a a t c t t t t t a t t

120 a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t a a a t t g t a a g c g t t a a t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a t t t a a c a t t c g c a a t t a

180 a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g

PsiI 240 g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g g c c g c c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c c g g c g

300 t a c a g g g c g c t c c c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g t t t c g g t g c a t g t c c c g c g a g g g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c a a a g c c a c g

360 g g g c c t c t t c g c t a t t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t c c c g g a g a a g c g a t a a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a

420 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward g g g t a a c g c c a g g g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g a g c g c g a c g c c c a t t g c g g t c c c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c t c g c g c t g c

480 T7 promoter AAV2 ITR T A A T A C G A C T C A C T A T A G G G T7 t a a t a c g a c t c a c t a t a g g g c g a a t t g t a g g c g g a a g g c c g t c a a g g c c g c a t g g c t g c g a t t a t g c t g a g t g a t a t c c c g c t t a a c a t c c g c c t t c c g g c a g t t c c g g c g t a c c g a c g c

540 AAV2 ITR c g c t c g c t c g c t c a c t g a g g c c g c c c g g g c a a a g c c c g g g c g t c g g g c g a c c t t t g g t c g g c g a g c g a g c g a g t g a c t c c g g c g g g c c c g t t t c g g g c c c g c a g c c c g c t g g a a a c c a g c

600 AAV2 ITR c c c g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a a c t c c a t c a c t a g g g g g g g c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t t g a g g t a g t g a t c c c c

660 AAV2 ITR t t c c t a t t g a c g t c a g a t c g c t a a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a a g g a t a a c t g c a g t c t a g c g a t t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c

720 a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a

780 piggyBac right (3') inverted repeat c a a g t g t a t c a t a c g t c a a g g c c g c a t t t a a c c c t a g a a a g a t a a t c a t a t t g t g a c g t a g t t c a c a t a g t a t g c a g t t c c g g c g t a a a t t g g g a t c t t t c t a t t a g t a t a a c a c t g c a t

840 piggyBac right (3') inverted repeat c g t t a a a g a t a a t c a t g t g t a a a a t t g a c g c a t g t g t t t t a t c g g t c t g t a t a t c g a g g t g c a a t t t c t a t t a g t a c a c a t t t t a a c t g c g t a c a c a a a a t a g c c a g a c a t a t a g c t c c a

900 t t a t t t a t t a a t t t g a a t a g a t a t t a a g t t t t a t t a t a t t t a c a c t t a c a t a c t a a t a a t a a t a a a t a a t t a a a c t t a t c t a t a a t t c a a a a t a a t a t a a a t g t g a a t g t a t g a t t a t t a

960 a a a t t c a a c a a a c a a t t t a t t t a t g t t t a t t t a t t t a t t a a a a a a a a c a a a a a c t c a a a a t t t a a g t t g t t t g t t a a a t a a a t a c a a a t a a a t a a a t a a t t t t t t t t g t t t t t g a g t t t t

PpuMI 1020 t t t c t t c t a t a a a g t a a c a a a a c t t t t a t c g a a t t c c t g c a g c c c a g c t t g a t c t t a g g t a a a g a a g a t a t t t c a t t g t t t t g a a a a t a g c t t a a g g a c g t c g g g t c g a a c t a g a a t c c a

XbaI 1080 EF-1 α promoter c c c t c g a c g a a t t c t c t a g a g a a g a g c a t g c g t g a g g c t c c g g t g c c c g t c a g t g g g c a g g g g a g c t g c t t a a g a g a t c t c t t c t c g t a c g c a c t c c g a g g c c a c g g g c a g t c a c c c g t c

MfeI 1140 EF-1 α promoter a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t t g a a c c g g t g t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a a c t t g g c c a c

1200 EF-1 α promoter c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g c t c c g c c t t t g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c g a g g c g g a a a

1260 EF-1 α promoter t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g t t c t t t t t c a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g c a a g a a a a a g

1320 EF-1 α promoter EF-1 α intron A g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g c g g g c c t g g c c c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c g c c c g g a c c g g

1380 EF-1 α promoter EF-1 α intron A *PGQTGQIVEVGRAATR t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c g c c c c t g g c t g c a g t a c a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g c g g g g a c c g a c g t c a t g

1440 EF-1 α promoter EF-1 α intron A SEQDRAEPQFHTPSNSAKRK g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a g g c c t t g c g c t c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c t c c g g a a c g c g a

1500 EF-1 α promoter EF-1 α intron A LLGKAEHKLQPRAQASPGGR t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c t t g g g c g c t g g g g c c g c c g c a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g a a c c c g c g a c c c c g g c g g c g

1560 EF-1 α promoter EF-1 α intron A AFRTAGERRDRQKRYTELWK g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t a g c c a t t c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a t c g g t a a

1620 EF-1 α promoter EF-1 α intron A FNKIVQQSAKKRALYDQLHP t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t a a a t g c g a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a t t t a c g c

PspOMIApaIBglII 1680 EF-1 α promoter EF-1 α intron A GLDACQYKPKQPRPRRRPGH g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g g g c c c g t c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c c c g g g c a

AflIIIPciI 1740 EF-1 α promoter EF-1 α intron A TGACMNPSAPGALAAVSFRV g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g a a t c g g a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c t t a g c c t

FseI 1800 EF-1 α promoter EF-1 α intron A PTTELQGAQEPAQGRAATYR c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c g t g t a t c g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g c a c a t a g

BstAPIPasI 1860 EF-1 α promoter EF-1 α intron A GARPPLAPGTPVLQTLPFIA g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a a a g a t g g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t t t c t a c c

1920 EF-1 α promoter EF-1 α intron A AERGQQLSSLISSAASPLAP c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c t c g g g a g a g c g g g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g a g c c c t c t c g c c

Bpu10IBbvCI 1980 EF-1 α promoter EF-1 α intron A PHTVWVFSFPRETRLRRKM g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c g c t t c a t g t c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g c a g c g a a g t a c a

2040 EF-1 α promoter EF-1 α intron A g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g a g c t t t t g g a g t c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c t c g a a a a c c t c a

DraIII 2100 EF-1 α promoter EF-1 α intron A a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c t g a g t g g t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g a c t c a c c

2160 EF-1 α promoter EF-1 α intron A g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t t g c c c t t c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a a c g g g a a

2220 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t t t t t c t t a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a a a a a g a a

2280 1 5 10 MVSKGEEVIK EF-1 α promoter tdTomato EF-1 α intron A MVSKGEEVIK *TDHFSRWHHALALLDDF c c a t t t c a g g t g t c g t g a a a a c t a c g c c a a t g g t g a g c a a g g g c g a g g a g g t c a t c a a a g g g t a a a g t c c a c a g c a c t t t t g a t g c g g t t a c c a c t c g t t c c c g c t c c t c c a g t a g t t t c

2340 15 20 25 30 EFMRFKVRMEGSMNGHEFEI tdTomato EFMRFKVRMEGSMNGHEFEI LEHAELHAHLAGHVAVLELD a g t t c a t g c g c t t c a a g g t g c g c a t g g a g g g c t c c a t g a a c g g c c a c g a g t t c g a g a t c g t c a a g t a c g c g a a g t t c c a c g c g t a c c t c c c g a g g t a c t t g c c g g t g c t c a a g c t c t a g c

2400 35 40 45 50 EGEGEGRPYEGTQAAKLKVT tdTomato EGEGEGRPYEGTQAAKLKVT LALALAAGVLAGLGGLQLHG a g g g c g a g g g c g a g g g c c g c c c c t a c g a g g g c a c c c a g g c c g c c a a g c t g a a g g t g a c c a t c c c g c t c c c g c t c c c g g c g g g g a t g c t c c c g t g g g t c c g g c g g t t c g a c t t c c a c t g g t

2460 55 60 65 70 KGGPLPFAWDILSPQFMYGS tdTomato KGGPLPFAWDILSPQFMYGS LAAGQGEGPVDQGGLEHVAG a g g g c g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g t c c c c c c a g t t c a t g t a c g g c t c c a t c c c g c c g g g g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g g g t c a a g t a c a t g c c g a g g t

2520 75 80 85 90 KAYVKHPADIPDYKKLSFPE tdTomato KAYVKHPADIPDYKKLSFPE LRVHLVGGVDGIVLLQGEGL a g g c g t a c g t g a a g c a c c c c g c c g a c a t c c c c g a t t a c a a g a a g c t g t c c t t c c c c g a g g t c c g c a t g c a c t t c g t g g g g c g g c t g t a g g g g c t a a t g t t c t t c g a c a g g a a g g g g c t c c

2580 95 100 105 110 GFKWERVMNFEDGGLVTVTQ tdTomato GFKWERVMNFEDGGLVTVTQ AELPLAHHVELVATQHGHGL g c t t c a a g t g g g a g c g c g t g a t g a a c t t c g a g g a c g g c g g t c t g g t g a c c g t g a c c c a g g c g a a g t t c a c c c t c g c g c a c t a c t t g a a g c t c c t g c c g c c a g a c c a c t g g c a c t g g g t c c

2640 115 120 125 130 DSSLQDGTLIYKVKMRGTNF tdTomato DSSLQDGTLIYKVKMRGTNF VGGQLVARQDVLHLHAAGVE a c t c c t c c c t g c a g g a c g g c a c g c t g a t c t a c a a g g t g a a g a t g c g c g g c a c c a a c t t c c t g a g g a g g g a c g t c c t g c c g t g c g a c t a g a t g t t c c a c t t c t a c g c g c c g t g g t t g a a g g

2700 135 140 145 150 PPDGPVMQKKTMGWEASTER tdTomato PPDGPVMQKKTMGWEASTER GGVAGYHLLLGHAPLGGGLA c c c c c g a c g g c c c c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c a c c g a g c g c c g g g g g c t g c c g g g g c a t t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g t g g c t c g c g g

2760 155 160 165 170 LYPRDGVLKGEIHQALKLKD tdTomato LYPRDGVLKGEIHQALKLKD QVGAVAHQLALDVLGQLQLV t g t a c c c c c g c g a c g g c g t g c t g a a g g g c g a g a t c c a c c a g g c c c t g a a g c t g a a g g a c g a c a t g g g g g c g c t g c c g c a c g a c t t c c c g c t c t a g g t g g t c c g g g a c t t c g a c t t c c t g c

2820 175 180 185 190 GGHYLVEFKTIYMAKKPVQL tdTomato GGHYLVEFKTIYMAKKPVQL AAVVQHLELGDVHGLLGHLQ g c g g c c a c t a c c t g g t g g a g t t c a a g a c c a t c t a c a t g g c c a a g a a g c c c g t g c a a c t g c c g c c g g t g a t g g a c c a c c t c a a g t t c t g g t a g a t g t a c c g g t t c t t c g g g c a c g t t g a c g

2880 195 200 205 210 PGYYYVDTKLDITSHNEDYT tdTomato PGYYYVDTKLDITSHNEDYT GAVVVHVGLQVDGGVVLVVG c c g g c t a c t a c t a c g t g g a c a c c a a g c t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a g g c c g a t g a t g a t g c a c c t g t g g t t c g a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t

2940 215 220 225 230 IVEQYERSEGRHHLFLGHGT tdTomato IVEQYERSEGRHHLFLGHGT DHFLVLAGLAAVVQEQPM t c g t g g a a c a g t a c g a g c g c t c c g a g g g c c g c c a c c a c c t g t t c c t g g g g c a t g g c a c c g a g c a c c t t g t c a t g c t c g c g a g g c t c c c g g c g g t g g t g g a c a a g g a c c c c g t a c c g t g g c

3000 235 240 245 250 GSTGSGSSGTASSEDNNMAV tdTomato GSTGSGSSGTASSEDNNMAV g c a g c a c c g g c a g c g g c a g c t c c g g c a c c g c c t c c t c c g a g g a c a a c a a c a t g g c c g t c a c g t c g t g g c c g t c g c c g t c g a g g c c g t g g c g g a g g a g g c t c c t g t t g t t g t a c c g g c a g t

3060 255 260 265 270 IKEFMRFKVRMEGSMNGHEF tdTomato IKEFMRFKVRMEGSMNGHEF t c a a a g a g t t c a t g c g c t t c a a g g t g c g c a t g g a g g g c t c c a t g a a c g g c c a c g a g t t c g a g t t t c t c a a g t a c g c g a a g t t c c a c g c g t a c c t c c c g a g g t a c t t g c c g g t g c t c a a g c

3120 275 280 285 290 EIEGEGEGRPYEGTQTAKLK tdTomato EIEGEGEGRPYEGTQTAKLK a g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c

3180 295 300 305 310 VTKGGPLPFAWDILSPQFMY tdTomato VTKGGPLPFAWDILSPQFMY t g a c c a a g g g c g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g t c c c c c c a g t t c a t g t a c g a c t g g t t c c c g c c g g g g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g g g t c a a g t a c a t g c

3240 315 320 325 330 GSKAYVKHPADIPDYKKLSF tdTomato GSKAYVKHPADIPDYKKLSF g c t c c a a g g c g t a c g t g a a g c a c c c c g c c g a c a t c c c c g a t t a c a a g a a g c t g t c c t t c c c g a g g t t c c g c a t g c a c t t c g t g g g g c g g c t g t a g g g g c t a a t g t t c t t c g a c a g g a a g g

3300 335 340 345 350 PEGFKWERVMNFEDGGLVTV tdTomato PEGFKWERVMNFEDGGLVTV c c g a g g g c t t c a a g t g g g a g c g c g t g a t g a a c t t c g a g g a c g g c g g t c t g g t g a c c g t g a g g c t c c c g a a g t t c a c c c t c g c g c a c t a c t t g a a g c t c c t g c c g c c a g a c c a c t g g c a c t

3360 355 360 365 370 TQDSSLQDGTLIYKVKMRGT tdTomato TQDSSLQDGTLIYKVKMRGT c c c a g g a c t c c t c c c t g c a g g a c g g c a c g c t g a t c t a c a a g g t g a a g a t g c g c g g c a c c a g g g t c c t g a g g a g g g a c g t c c t g c c g t g c g a c t a g a t g t t c c a c t t c t a c g c g c c g t g g t

3420 375 380 385 390 NFPPDGPVMQKKTMGWEAST tdTomato NFPPDGPVMQKKTMGWEAST a c t t c c c c c c c g a c g g c c c c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c a c c g t g a a g g g g g g g c t g c c g g g g c a t t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g t g g c

3480 395 400 405 410 ERLYPRDGVLKGEIHQALKL tdTomato ERLYPRDGVLKGEIHQALKL a g c g c c t g t a c c c c c g c g a c g g c g t g c t g a a g g g c g a g a t c c a c c a g g c c c t g a a g c t g a t c g c g g a c a t g g g g g c g c t g c c g c a c g a c t t c c c g c t c t a g g t g g t c c g g g a c t t c g a c t

3540 415 420 425 430 KDGGHYLVEFKTIYMAKKPV tdTomato KDGGHYLVEFKTIYMAKKPV a g g a c g g c g g c c a c t a c c t g g t g g a g t t c a a g a c c a t c t a c a t g g c c a a g a a g c c c g t g c t c c t g c c g c c g g t g a t g g a c c a c c t c a a g t t c t g g t a g a t g t a c c g g t t c t t c g g g c a c g

3600 435 440 445 450 QLPGYYYVDTKLDITSHNED tdTomato QLPGYYYVDTKLDITSHNED a a c t g c c c g g c t a c t a c t a c g t g g a c a c c a a g c t g g a c a t c a c c t c c c a c a a c g a g g a c t t t g a c g g g c c g a t g a t g a t g c a c c t g t g g t t c g a c c t g t a g t g g a g g g t g t t g c t c c t g a

3660 455 460 465 470 YTIVEQYERSEGRHHLFLYG tdTomato YTIVEQYERSEGRHHLFLYG a c a c c a t c g t g g a a c a g t a c g a g c g c t c c g a g g g c c g c c a c c a c c t g t t c c t g t a c g g c a t g t g g t a g c a c c t t g t c a t g c t c g c g a g g c t c c c g g c g g t g g t g g a c a a g g a c a t g c c g t

PspXIBspDIClaIBsrGI 3720 475 MDELYK* tdTomato MDELYK* t g g a c g a g c t g t a c a a g t a a t c g a t a a c a a c t c g a g t a c c g g c t t a a g t g a t a g a t c g g a a c c t g c t c g a c a t g t t c a t t a g c t a t t g t t g a g c t c a t g g c c g a a t t c a c t a t c t a g c c t

SgrAIMreIPmeIBsaI 3780 a g a g c g g t t c a g c a g g a a t g c c g a g a c c g g t t t a a a c t c a g t g t c c a t a g c g a c g c c g g c t c t c g c c a a g t c g t c c t t a c g g c t c t g g c c a a a t t t g a g t c a c a g g t a t c g c t g c g g c c g

3840 g c g a t a a g c t a t c t a t a a c a a g a a a a t a t a t a t a t a a t a a g t t a t c a c g t a a g t a g a a c a c g c t a t t c g a t a g a t a t t g t t c t t t t a t a t a t a t a t t a t t c a a t a g t g c a t t c a t c t t g t

3900 t g a a a t a a c a a t a t a a t t a t c g t a t g a g t t a a a t c t t a a a a g t c a c g t a a a a g a t a a t c a a c t t t a t t g t t a t a t t a a t a g c a t a c t c a a t t t a g a a t t t t c a g t g c a t t t t c t a t t a g t

3960 t g c g t c a t t t t g a c t c a c g c g g t c g t t a t a g t t c a a a a t c a g t g a c a c t t a c c g c a t t g a a c g c a g t a a a a c t g a g t g c g c c a g c a a t a t c a a g t t t t a g t c a c t g t g a a t g g c g t a a c t

4020 c a a g c a c g c c t c a c g g g a g c t c c a a g c g g c g a c t g a g a t g t c c t a a a t g c a c a g c g a c g g g t t c g t g c g g a g t g c c c t c g a g g t t c g c c g c t g a c t c t a c a g g a t t t a c g t g t c g c t g c c

NsiI 4080 a t t c g c g c t a t t t a g a a a g a g a g a g c a a t a t t t c a a g a a t g c a t g c g t c a a t t t t a c g c a t a a g c g c g a t a a a t c t t t c t c t c t c g t t a t a a a g t t c t t a c g t a c g c a g t t a a a a t g c g t

4140 g a c t a t c t t t g g a a g g t t a a g a g c t c a t c t g a g a t g c a g g t a c a t c c c a c t g a t g a g t c c c t g a t a g a a a c c t t c c a a t t c t c g a g t a g a c t c t a c g t c c a t g t a g g g t g a c t a c t c a g g

EagINotI 4200 c a a a t a g g a c g a a a c g c g c t t c g g t g c g t c t g g g a t t c c a c t g c t a t c c a c g c g g c c g c a g t t t a t c c t g c t t t g c g c g a a g c c a c g c a g a c c c t a a g g t g a c g a t a g g t g c g c c g g c g t

4260 c g g c a t t a c g t t a g a c g t c t a g g c c a c a c c a c c c t g a a g g c g c c t g c t c g c c t c t g a t c t g c c g t a a t g c a a t c t g c a g a t c c g g t g t g g t g g g a c t t c c g c g g a c g a g c g g a g a c t a g a

BlpI 4320 g t t g a a g c t a a g c a g g g t c g g t c c t g g t t a g t a c t t g g a t g g g a c t c c g c c t g g t a a t a g c a a c t t c g a t t c g t c c c a g c c a g g a c c a a t c a t g a a c c t a c c c t g a g g c g g a c c a t t a t c

Acc65IKpnI 4380 c c g g t a c c g t a g g c t t g c a t g g t c a t a g c t g t t t c c t t g c g c t g g g g a t g c g g t g g g c t c g g c c a t g g c a t c c g a a c g t a c c a g t a t c g a c a a a g g a a c g c g a c c c c t a c g c c a c c c g a g

4440 AAV2 ITR t a t a g a g a g t c g a t c g t g t g a t c g a a g g a a c c c c t a g t g a t g g a g t t g g c c a c t c c c t c t a t a t c t c t c a g c t a g c a c a c t a g c t t c c t t g g g g a t c a c t a c c t c a a c c g g t g a g g g a g a

4500 AAV2 ITR c t g c g c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c c g a c g c c c g g g c t t t g a c g c g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g g c t g c g g g c c c g a a a

4560 AAV2 ITR g c c c g g g c g g c c t c a g t g a g c g a g c g a g c g c g c a g c a t g t a g a g a c t g g g c c t c a t g g g c c g g g c c c g c c g g a g t c a c t c g c t c g c t c g c g c g t c g t a c a t c t c t g a c c c g g a g t a c c c g

4620 c t t c c g c c t a t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a c a g a a g g c g g a t a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t g t

4680 t g g t c a t a g c t g t t t c c t t g c g t a t t g g g c g c t c t c c g c t t c c t c g c t c a c t g a c t c g c t a c c a g t a t c g a c a a a g g a a c g c a t a a c c c g c g a g a g g c g a a g g a g c g a g t g a c t g a g c g a

4740 rrnB T1 terminator g c g c t c g g t c g t t c g g g t a a a g c c t g g g g t g c c t a a a t a a a a c g a a a g g c c c a g t c t t c c c g c g a g c c a g c a a g c c c a t t t c g g a c c c c a c g g a t t t a t t t t g c t t t c c g g g t c a g a a g g

4800 rrnB T1 terminator g a c t g a g c c t t t c g t t t t a t t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c t g a c t c g g a a a g c a a a a t a a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c

4860 ori c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g

4920 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c

4980 ori g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a

5040 ori t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c

5100 ori t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a

5160 ori g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g

5220 ori t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a

5280 ori t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g

5340 ori t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t

5400 ori c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a

5460 ori c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g

5520 g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a

5580 *W AmpR *W a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g

5640 285 280 275 270 HKILSAGIEAIQRNREDMTA AmpR HKILSAGIEAIQRNREDMTA a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c

5700 265 260 255 250 QSGTTYIVVIRSPKGDPGLA AmpR QSGTTYIVVIRSPKGDPGLA c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c

BpmI 5760 245 240 235 230 AIIGRSGREGAGSKDAIFWG AmpR AIIGRSGREGAGSKDAIFWG c t g c a a t g a t a c c g c g a g a a c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c g a c g t t a c t a t g g c g c t c t t g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g

5820 225 220 215 210 APLASRLLPGAVKDAEMWDI AmpR APLASRLLPGAVKDAEMWDI c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t

5880 205 200 195 190 LQQRSALTLLEGTLLKRLTT AmpR LQQRSALTLLEGTLLKRLTT t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c

5940 185 180 175 170 AMAVPMTTDREDNPIAENLE AmpR AMAVPMTTDREDNPIAENLE t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a

6000 165 160 155 150 PEWRDLRTVHDGMNHLFATL AmpR PEWRDLRTVHDGMNHLFATL c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t

6060 145 140 135 130 EKPGGITTLLLNAATNDSMT AmpR EKPGGITTLLLNAATNDSMT g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c

6120 125 120 115 110 IAASCLERVTMGDTLHKETV AmpR IAASCLERVTMGDTLHKETV t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t

6180 105 100 95 90 PSYEVLDNQSYHIRRGLQEQ AmpR PSYEVLDNQSYHIRRGLQEQ c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a

6240 85 80 75 70 GADIRSLVAGCLLVKFTSMM AmpR A T A A T A C C G C G C C A C A T A G C Amp-R GADIRSLVAGCLLVKFTSMM g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t

6300 65 60 55 50 PFREEPRFSELIKGSNLDLE AmpR PFREEPRFSELIKGSNLDLE t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a

6360 45 40 35 30 IYGVRAGLQDEADKVKVLTE AmpR IYGVRAGLQDEADKVKVLTE c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a

6420 25 PH 20 15 10 signal sequence AFVPLCFAAFFPILAVRF AmpR PHAFVPLCFAAFFPILAVRF c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t

6480 5 1 signal sequence HQISM AmpR AmpR promoter HQISM a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a

6506 AmpR promoter g t c t c a t g a g c g g a t a c a t a t t t g a a c a g a g t a c t c g c c t a t g t a t a a a c t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.