Addgene pCDNA6-IgK-hFRa(23-234)C11S-th-Fc Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCDNA6-IgK-hFRa(23-234)C11S-th-Fc Sequencing Result


Map View

F1ori-F (6521 .. 6542) F1ori-R (6311 .. 6330) BGH-rev (5948 .. 5965) PmeI (5932) 6xHis AgeI (5902) BstBI (5852) XbaI (5839) BsrGI (5515) EcoNI (5400) SacII (5346) BmgBI (5267) AleI (5253) KpnI (5098) Acc65I (5094) PstI (5084) XcmI (5060) PasI (5042) BamHI (4738) EcoRI (4452) pMT2-F (4423 .. 4442) BmtI (4305) NheI (4301) T7 (4269 .. 4288) SacI (4224) Eco53kI (4222) CMV-F (4175 .. 4195) SnaBI (3996) NdeI (3890) CMV enhancer SpeI (3655) Bpu10I (3586) MfeI (3567) pRS-marker (3450 .. 3469) BglII (3418) SgrDI (3404) pBABE 3' (75 .. 95) SV40 promoter SV40pro-F (322 .. 341) StuI (393) AvrII (394) MscI (526) EagI (639) PpuMI (682) BsaBI * (821) BlpI (918) SV40pA-R (1116 .. 1135) EBV-rev (1170 .. 1189) BstZ17I (1211) M13 Reverse (1249 .. 1265) lac operator M13/pUC Reverse (1262 .. 1284) CAP binding site L4440 (1480 .. 1497) PciI (1590) pBR322ori-F (1731 .. 1750) ScaI (2963) Amp-R (3033 .. 3052) SspI (3287) pCDNA6-IgK-hFRa(23-234)C11S-th-Fc 6586 bp

Sequence View

5ʹ 3ʹ 60 f1 ori g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c

120 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g

180 SV40 promoter a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g

240 SV40 promoter a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a

300 SV40 promoter SV40 ori c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g

360 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c t g a g c t g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g a c t c g a

StuIAvrII 420 SV40 promoter SV40 ori a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c g g g t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g c c c

480 EM7 promoter a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c a c g t g t t g a c a a t t a a t c a t c g g c a t a g t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c g t g c a c a a c t g t t a a t t a g t a g c c g t a t c

MscI 540 1 5 MAKPLS EM7 promoter BSD t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g g a a c t a a a c c a t g g c c a a g c c t t t g t c t a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t c c t t g a t t t g g t a c c g g t t c g g a a a c a g a

600 10 15 20 25 QEESTLIERATATINSIPIS BSD c a a g a a g a a t c c a c c c t c a t t g a a a g a g c a a c g g c t a c a a t c a a c a g c a t c c c c a t c t c t g t t c t t c t t a g g t g g g a g t a a c t t t c t c g t t g c c g a t g t t a g t t g t c g t a g g g g t a g a g a

EagI 660 30 35 40 45 EDYSVASAALSSDGRIFTGV BSD g a a g a c t a c a g c g t c g c c a g c g c a g c t c t c t c t a g c g a c g g c c g c a t c t t c a c t g g t g t c c t t c t g a t g t c g c a g c g g t c g c g t c g a g a g a g a t c g c t g c c g g c g t a g a a g t g a c c a c a g

PpuMI 720 50 55 60 65 NVYHFTGGPCAELVVLGTAA BSD a a t g t a t a t c a t t t t a c t g g g g g a c c t t g t g c a g a a c t c g t g g t g c t g g g c a c t g c t g c t t t a c a t a t a g t a a a a t g a c c c c c t g g a a c a c g t c t t g a g c a c c a c g a c c c g t g a c g a c g a

780 70 75 80 85 AAAAGNLTCIVAIGNENRGI BSD g c t g c g g c a g c t g g c a a c c t g a c t t g t a t c g t c g c g a t c g g a a a t g a g a a c a g g g g c a t c c g a c g c c g t c g a c c g t t g g a c t g a a c a t a g c a g c g c t a g c c t t t a c t c t t g t c c c c g t a g

BsaBI* 840 90 95 100 105 LSPCGRCRQVLLDLHPGIKA BSD t t g a g c c c c t g c g g a c g g t g c c g a c a g g t g c t t c t c g a t c t g c a t c c t g g g a t c a a a g c c a a c t c g g g g a c g c c t g c c a c g g c t g t c c a c g a a g a g c t a g a c g t a g g a c c c t a g t t t c g g

900 110 115 120 125 IVKDSDGQPTAVGIRELLPS BSD a t a g t g a a g g a c a g t g a t g g a c a g c c g a c g g c a g t t g g g a t t c g t g a a t t g c t g c c c t c t t a t c a c t t c c t g t c a c t a c c t g t c g g c t g c c g t c a a c c c t a a g c a c t t a a c g a c g g g a g a

BlpI 960 130 GYVWEG* BSD g g t t a t g t g t g g g a g g g c t a a g c a c t t c g t g g c c g a g g a g c a g g a c t g a c a c g t g c t a c g c c a a t a c a c a c c c t c c c g a t t c g t g a a g c a c c g g c t c c t c g t c c t g a c t g t g c a c g a t g c

1020 a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t

1080 SV40 poly(A) signal c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t

1140 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t

1200 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t

BstZ17I 1260 M13 rev C A G T A T C G A C A A M13 Reverse t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a

1320 -10 M13 rev lac operator lac promoter A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t

1380 -35 lac promoter CAP binding site g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a

1440 g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g

1500 G A G C G A G T G A C T G A G C G A L4440 g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c

1560 c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g

PciI 1620 c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c

1680 ori g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t

1740 ori T T T C T A T G G T pBR322ori-F t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t

1800 ori C C G C A A A G G G pBR322ori-F g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c

1860 ori a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c

1920 ori g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a

1980 ori t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t

2040 ori c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c

2100 ori c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a

2160 ori t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g

2220 ori c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c

2280 ori c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c

2340 g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t

2400 g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c

2460 285 280 275 *WHKILSAGIEAIQRNR AmpR *WHKILSAGIEAIQRNR g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c

2520 270 265 260 255 EDMTAQSGTTYIVVIRSPKG AmpR EDMTAQSGTTYIVVIRSPKG t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g

2580 250 245 240 235 DPGLAAIIGRSGREGAGSKD AmpR DPGLAAIIGRSGREGAGSKD a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g

2640 230 225 220 215 AIFWGAPLASRLLPGAVKDA AmpR AIFWGAPLASRLLPGAVKDA a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g

2700 210 205 200 195 EMWDILQQRSALTLLEGTLL AmpR EMWDILQQRSALTLLEGTLL c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c

2760 190 185 180 175 KRLTTAMAVPMTTDREDNPI AmpR KRLTTAMAVPMTTDREDNPI t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a

2820 170 165 160 155 AENLEPEWRDLRTVHDGMNH AmpR AENLEPEWRDLRTVHDGMNH g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c

2880 150 145 140 135 LFATLEKPGGITTLLLNAAT AmpR LFATLEKPGGITTLLLNAAT c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a

2940 130 125 120 115 NDSMTIAASCLERVTMGDTL AmpR NDSMTIAASCLERVTMGDTL g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c

ScaI 3000 110 105 100 95 HKETVPSYEVLDNQSYHIRR AmpR HKETVPSYEVLDNQSYHIRR a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c

3060 90 85 80 75 GLQEQGADIRSLVAGCLLVK AmpR A T A A T A C C G C G C C A C A T A G C Amp-R GLQEQGADIRSLVAGCLLVK a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a

3120 70 65 60 55 FTSMMPFREEPRFSELIKGS AmpR FTSMMPFREEPRFSELIKGS a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a

3180 50 45 40 35 NLDLEIYGVRAGLQDEADKV AmpR NLDLEIYGVRAGLQDEADKV g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g

3240 30 25 KVLTEPH 20 15 signal sequence AFVPLCFAAFFPI AmpR KVLTEPHAFVPLCFAAFFPI t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a

SspI 3300 10 5 1 signal sequence LAVRFHQISM AmpR AmpR promoter LAVRFHQISM a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a

3360 AmpR promoter t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t

BglIISgrDI 3420 AmpR promoter a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c g a c g g a t c g g g a g a t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g c t g c c t a g c c c t c t

3480 A T G T T A G A C G A G A C T A C G G C pRS-marker t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g

3540 c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t

Bpu10IMfeI 3600 g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a

SpeI 3660 CMV enhancer t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a g t a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t c a

3720 CMV enhancer t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a

3780 CMV enhancer a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c

3840 CMV enhancer t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c

NdeI 3900 CMV enhancer g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a

3960 CMV enhancer a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c

SnaBI 4020 CMV enhancer a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c

4080 CMV promoter g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a

4140 CMV promoter c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g

4200 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c

Eco53kISacI 4260 CMV promoter t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a

BmtINheI 4320 1 MGW T7 promoter IgH signal sequence 1 MGW IgH signal sequence Kozak sequence T A A T A C G A C T C A C T A T A G G G T7 a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g c g c c a c c a t g g g a t g t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a c c g a t c g c g g t g g t a c c c t a c

4380 5 10 15 SCIILFLVATAT IgH signal sequence 5 10 15 SCIILFLVATAT IgH signal sequence g a g c t g t a t c a t c c t c t t c t t g g t a g c a a c a g c t a c a g g t a a g g g g c t c a c a g t a g c a g g c t c g a c a t a g t a g g a g a a g a a c c a t c g t t g t c g a t g t c c a t t c c c c g a g t g t c a t c g t c c

4440 IgH signal sequence IgH signal sequence T T G C C T T T C T C T C C A C A G pMT2-F MGDNDIHFAFLST c t t g a g g t c t g g a c a t a t a t a t g g g t g a c a a t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g a a c t c c a g a c c t g t a t a t a t a c c c a c t g t t a c t g t a g g t g a a a c g g a a a g a g a g g t g t c

EcoRI 4500 GVHS (in frame with IgH signal sequence) EFQTRIAWARTELLNV IgH signal sequence GVHS (in frame with IgH signal sequence) EFQTRIAWARTELLNV IgH signal sequence G T pMT2-F GVHSEFQTRIAWARTELLNV g t g t c c a c t c c g a a t t c c a g a c a a g g a t t g c a t g g g c c a g g a c t g a g c t t c t c a a t g t c t c a c a g g t g a g g c t t a a g g t c t g t t c c t a a c g t a c c c g g t c c t g a c t c g a a g a g t t a c a g a

4560 (in frame with IgH signal sequence) CMNAKHHKEKPGPEDKLHEQ (in frame with IgH signal sequence) CMNAKHHKEKPGPEDKLHEQ CMNAKHHKEKPGPEDKLHEQ g c a t g a a c g c c a a g c a c c a c a a g g a a a a g c c a g g c c c c g a g g a c a a g t t g c a t g a g c a g t c g t a c t t g c g g t t c g t g g t g t t c c t t t t c g g t c c g g g g c t c c t g t t c a a c g t a c t c g t c a

4620 (in frame with IgH signal sequence) CRPWRKNACCSTNTSQEAHK (in frame with IgH signal sequence) CRPWRKNACCSTNTSQEAHK CRPWRKNACCSTNTSQEAHK g t c g a c c c t g g a g g a a g a a t g c c t g c t g t t c t a c c a a c a c c a g c c a g g a a g c c c a t a a g g c a g c t g g g a c c t c c t t c t t a c g g a c g a c a a g a t g g t t g t g g t c g g t c c t t c g g g t a t t c c

4680 (in frame with IgH signal sequence) DVSYLYRFNWNHCGEMAPAC (in frame with IgH signal sequence) DVSYLYRFNWNHCGEMAPAC DVSYLYRFNWNHCGEMAPAC a t g t t t c c t a c c t a t a t a g a t t c a a c t g g a a c c a c t g t g g a g a g a t g g c a c c t g c c t g c a t a c a a a g g a t g g a t a t a t c t a a g t t g a c c t t g g t g a c a c c t c t c t a c c g t g g a c g g a c g t

BamHI 4740 (in frame with IgH signal sequence) KRHFIQDTCLYECSPNLGPW (in frame with IgH signal sequence) KRHFIQDTCLYECSPNLGPW KRHFIQDTCLYECSPNLGPW a a c g g c a t t t c a t c c a g g a c a c c t g c c t c t a c g a g t g c t c c c c c a a c t t g g g g c c c t g g a t t g c c g t a a a g t a g g t c c t g t g g a c g g a g a t g c t c a c g a g g g g g t t g a a c c c c g g g a c c t

4800 (in frame with IgH signal sequence) IQQVDQSWRKERVLNVPLCK (in frame with IgH signal sequence) IQQVDQSWRKERVLNVPLCK IQQVDQSWRKERVLNVPLCK t c c a g c a g g t g g a t c a g a g c t g g c g c a a a g a g c g g g t a c t g a a c g t g c c c c t g t g c a a a g a g g t c g t c c a c c t a g t c t c g a c c g c g t t t c t c g c c c a t g a c t t g c a c g g g g a c a c g t t t c

4860 (in frame with IgH signal sequence) EDCEQWWEDCRTSYTCKSNW (in frame with IgH signal sequence) EDCEQWWEDCRTSYTCKSNW EDCEQWWEDCRTSYTCKSNW a g g a c t g t g a g c a a t g g t g g g a a g a t t g t c g c a c c t c c t a c a c c t g c a a g a g c a a c t g g c t c c t g a c a c t c g t t a c c a c c c t t c t a a c a g c g t g g a g g a t g t g g a c g t t c t c g t t g a c c g

4920 (in frame with IgH signal sequence) HKGWNWTSGFNKCAVGAACQ (in frame with IgH signal sequence) HKGWNWTSGFNKCAVGAACQ HKGWNWTSGFNKCAVGAACQ a c a a g g g c t g g a a c t g g a c t t c a g g g t t t a a c a a g t g c g c a g t g g g a g c t g c c t g c c a a c t g t t c c c g a c c t t g a c c t g a a g t c c c a a a t t g t t c a c g c g t c a c c c t c g a c g g a c g g t t g

4980 (in frame with IgH signal sequence) PFHFYFPTPTVLCNEIWTHS (in frame with IgH signal sequence) PFHFYFPTPTVLCNEIWTHS PFHFYFPTPTVLCNEIWTHS c t t t c c a t t t c t a c t t c c c c a c a c c c a c t g t t c t g t g c a a t g a a a t c t g g a c t c a c t c c t g a a a g g t a a a g a t g a a g g g g t g t g g g t g a c a a g a c a c g t t a c t t t a g a c c t g a g t g a g g a

5040 (in frame with IgH signal sequence) YKVSNYSRGSGRCIQMWFDP (in frame with IgH signal sequence) YKVSNYSRGSGRCIQMWFDP YKVSNYSRGSGRCIQMWFDP a c a a g g t c a g c a a c t a c a g c c g a g g g a g t g g c c g c t g c a t c c a g a t g t g g t t c g a c c c a g t g t t c c a g t c g t t g a t g t c g g c t c c c t c a c c g g c g a c g t a g g t c t a c a c c a a g c t g g g t c

Acc65IKpnIPstIXcmIPasI 5100 (in frame with IgH signal sequence) AQGNPNEEVARFYAAAMSGT (in frame with IgH signal sequence) AQGNPNEEVARFYAAAMSGT thrombin site AQGNPNEEVARFYAAAMSGT c c c a g g g c a a c c c c a a t g a g g a g g t g g c g a g g t t c t a t g c t g c a g c c a t g a g t g g t a c c c g g g t c c c g t t g g g g t t a c t c c t c c a c c g c t c c a a g a t a c g a c g t c g g t a c t c a c c a t g g g

5160 (in frame with IgH signal sequence) LVPRGSEPKSSDKTHTCPPC 1 5 LVPRGS (in frame with thrombin site) EPKSSDKTHTCPPC thrombin site LVPRGSEPKSSDKTHTCPPC t g g t t c c g c g t g g t t c c g a g c c c a a a t c t a g t g a c a a a a c t c a c a c a t g c c c a c c g t g c c a c c a a g g c g c a c c a a g g c t c g g g t t t a g a t c a c t g t t t t g a g t g t g t a c g g g t g g c a c g g

5220 (in frame with IgH signal sequence) PAPELLGGPSVFLFPPKPKD (in frame with thrombin site) PAPELLGGPSVFLFPPKPKD PAPELLGGPSVFLFPPKPKD c a g c a c c t g a a c t c c t g g g g g g a c c g t c a g t c t t c c t c t t c c c c c c a a a a c c c a a g g a c a g t c g t g g a c t t g a g g a c c c c c c t g g c a g t c a g a a g g a g a a g g g g g g t t t t g g g t t c c t g t

BmgBIAleI 5280 (in frame with IgH signal sequence) TLMISRTPEVTCVVVDVSHE (in frame with thrombin site) TLMISRTPEVTCVVVDVSHE TLMISRTPEVTCVVVDVSHE c c c t c a t g a t c t c c c g g a c c c c t g a g g t c a c a t g c g t g g t g g t g g a c g t g a g c c a c g a a g g g g a g t a c t a g a g g g c c t g g g g a c t c c a g t g t a c g c a c c a c c a c c t g c a c t c g g t g c t t c

5340 (in frame with IgH signal sequence) DPEVKFNWYVDGVEVHNAKT (in frame with thrombin site) DPEVKFNWYVDGVEVHNAKT DPEVKFNWYVDGVEVHNAKT a c c c t g a g g t c a a g t t c a a c t g g t a c g t g g a c g g c g t g g a g g t g c a t a a t g c c a a g a c a a t g g g a c t c c a g t t c a a g t t g a c c a t g c a c c t g c c g c a c c t c c a c g t a t t a c g g t t c t g t t

SacII 5400 (in frame with IgH signal sequence) KPREEQYNSTYRVVSVLTVL (in frame with thrombin site) KPREEQYNSTYRVVSVLTVL KPREEQYNSTYRVVSVLTVL a g c c g c g g g a g g a g c a g t a c a a c a g c a c g t a c c g t g t g g t c a g c g t c c t c a c c g t c c t g c t c g g c g c c c t c c t c g t c a t g t t g t c g t g c a t g g c a c a c c a g t c g c a g g a g t g g c a g g a c g

EcoNI 5460 (in frame with IgH signal sequence) HQDWLNGKEYKCKVSNKALP (in frame with thrombin site) HQDWLNGKEYKCKVSNKALP HQDWLNGKEYKCKVSNKALP a c c a g g a c t g g c t g a a t g g c a a g g a g t a c a a g t g c a a g g t c t c c a a c a a a g c c c t c c c a g t g g t c c t g a c c g a c t t a c c g t t c c t c a t g t t c a c g t t c c a g a g g t t g t t t c g g g a g g g t c

BsrGI 5520 (in frame with IgH signal sequence) APIEKTISKAKGQPREPQVY (in frame with thrombin site) APIEKTISKAKGQPREPQVY APIEKTISKAKGQPREPQVY c c c c c a t c g a g a a a a c c a t c t c c a a a g c c a a a g g g c a g c c c c g a g a a c c a c a g g t g t a c a g g g g g t a g c t c t t t t g g t a g a g g t t t c g g t t t c c c g t c g g g g c t c t t g g t g t c c a c a t g t

5580 (in frame with IgH signal sequence) TLPPSRDELTKNQVSLTCLV (in frame with thrombin site) TLPPSRDELTKNQVSLTCLV TLPPSRDELTKNQVSLTCLV c c c t g c c c c c a t c c c g g g a t g a g c t g a c c a a g a a c c a g g t c a g c c t g a c c t g c c t g g t c a g g g a c g g g g g t a g g g c c c t a c t c g a c t g g t t c t t g g t c c a g t c g g a c t g g a c g g a c c a g t

5640 (in frame with IgH signal sequence) KGFYPSDIAVEWESNGQPEN (in frame with thrombin site) KGFYPSDIAVEWESNGQPEN KGFYPSDIAVEWESNGQPEN a a g g c t t c t a t c c c a g c g a c a t c g c c g t g g a g t g g g a g a g c a a t g g g c a g c c g g a g a a c a t t c c g a a g a t a g g g t c g c t g t a g c g g c a c c t c a c c c t c t c g t t a c c c g t c g g c c t c t t g t

5700 (in frame with IgH signal sequence) NYKATPPVLDSDGSFFLYSK (in frame with thrombin site) NYKATPPVLDSDGSFFLYSK NYKATPPVLDSDGSFFLYSK a c t a c a a g g c c a c g c c t c c c g t g c t g g a c t c c g a c g g c t c c t t c t t c c t c t a c a g c a a g c t g a t g t t c c g g t g c g g a g g g c a c g a c c t g a g g c t g c c g a g g a a g a a g g a g a t g t c g t t c g

5760 (in frame with IgH signal sequence) LTVDKSRWQQGNVFSCSVMH (in frame with thrombin site) LTVDKSRWQQGNVFSCSVMH LTVDKSRWQQGNVFSCSVMH t c a c c g t g g a c a a g a g c a g g t g g c a g c a g g g g a a c g t c t t c t c a t g c t c c g t g a t g c a t g a g t g g c a c c t g t t c t c g t c c a c c g t c g t c c c c t t g c a g a a g a g t a c g a g g c a c t a c g t a c

5820 (in frame with IgH signal sequence) EALHNHYTQKSLSLSPGKHH (in frame with thrombin site) EALHNHYTQKSLSLSPGK 1 HH 6xHis EALHNHYTQKSLSLSPGKHH a g g c t c t g c a c a a c c a c t a c a c g c a g a a g a g c c t c t c c c t g t c t c c g g g t a a a c a t c a c c t c c g a g a c g t g t t g g t g a t g t g c g t c t t c t c g g a g a g g g a c a g a g g c c c a t t t g t a g t g g

BstBIXbaI 5880 HHHH* 1 5 GKPIPNPL V5 tag 5 HHHH * 6xHis HHHH* a c c a t c a c c a t t g a t a a t t c t a g a g g g c c c t t c g a a g g t a a g c c t a t c c c t a a c c c t c t c t g g t a g t g g t a a c t a t t a a g a t c t c c c g g g a a g c t t c c a t t c g g a t a g g g a t t g g g a g a g

PmeIAgeI 5940 10 LGLDST RTG 1 5 HHHHHH * V5 tag 6xHis c t c g g t c t c g a t t c t a c g c g t a c c g g t c a t c a t c a c c a t c a c c a t t g a g t t t a a a c c c g c g a g c c a g a g c t a a g a t g c g c a t g g c c a g t a g t a g t g g t a g t g g t a a c t c a a a t t t g g g c g

6000 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c

6060 bGH poly(A) signal c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a

6120 bGH poly(A) signal g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g

6180 bGH poly(A) signal a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a

6240 f1 ori t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g

6300 f1 ori g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g

6360 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g

6420 f1 ori c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g

6480 f1 ori g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c

6540 f1 ori G T G G A C T C T T G T T C C A A A C T F1ori-F g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a

3ʹ 5ʹ 6586 f1 ori G G F1ori-F g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.