Addgene pGal4AD-CIBN Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pGal4AD-CIBN Sequencing Result

Map View

800 600 400 200 End (818) Esp3I - BsmBI (805) NaeI (720) NgoMIV (718) BanII - Bsp1286I (678) DrdI (655) NspI (654) PciI (650) TaqII (645) BstEII (625) EcoRI (601) T7 (573 .. 592) MflI * - BglII - BstYI (566) BstXI (504) MslI (502) MluI (464) CsiI - SexAI * (435) MmeI (421) AclI (359) MfeI (344) AfeI (332) RsrII (295) KpnI (221) BanI Acc65I (217) EarI (193) AvaI - BsoBI (181) XmnI (176) HindIII (147) BsgI (90) BfuAI - BspMI (47) Start (0) SV40 NLS GAL4 activation domain T7 promoter pGal4AD-CIBN 818 bp

Sequence View

BspMIBfuAIStart (0) 5ʹ 3ʹ 60 t a a a a a a g t t t g c t g t c t t g c t a t c n a g t a t a a a t a g a c c t g c a a t t a t t a a t c t t t t g t a t t t t t t c a a a c g a c a g a a c g a t a g n t c a t a t t t a t c t g g a c g t t a a t a a t t a g a a a a c a

BsgI 120 t t c c t c g t c a t t g t t c t c g t t c c c t t t c t t c c t t g t t t c t t t t t c t g c a c a a t a t t t c a a a a g g a g c a g t a a c a a g a g c a a g g g a a a g a a g g a a c a a a g a a a a a g a c g t g t t a t a a a g t t

XmnIHindIII 180 MDKAELI g c t a t a c c a a g c a t a c a a t c a a c t c c a a g c t t t g c a a a g a t g g a t a a a g c g g a a t t a a t t c g a t a t g g t t c g t a t g t t a g t t g a g g t t c g a a a c g t t t c t a c c t a t t t c g c c t t a a t t a a

Acc65IBanIKpnIEarIBsoBIAvaI 240 130 PKKKRKV (in frame with SV40 NLS) ELGTA 770 ANFNQ SV40 NLS GAL4 activation domain PEPPKKKRKVELGTAANFNQ c c c g a g c c t c c a a a a a a g a a g a g a a a g g t c g a a t t g g g t a c c g c c g c c a a t t t t a a t c a a g g g c t c g g a g g t t t t t t c t t c t c t t t c c a g c t t a a c c c a t g g c g g c g g t t a a a a t t a g t t

RsrII 300 775 780 785 790 SGNIADSSLSFTFTNSSNGP GAL4 activation domain SGNIADSSLSFTFTNSSNGP a g t g g g a a t a t t g c t g a t a g c t c a t t g t c c t t c a c t t t c a c t a a c a g t a g c a a c g g t c c g t c a c c c t t a t a a c g a c t a t c g a g t a a c a g g a a g t g a a a g t g a t t g t c a t c g t t g c c a g g c

AclIMfeIAfeI 360 795 800 805 810 NLITTQTNSQALSQPIASSN GAL4 activation domain NLITTQTNSQALSQPIASSN a a c c t c a t a a c a a c t c a a a c a a a t t c t c a a g c g c t t t c a c a a c c a a t t g c c t c c t c t a a c t t g g a g t a t t g t t g a g t t t g t t t a a g a g t t c g c g a a a g t g t t g g t t a a c g g a g g a g a t t g

420 815 820 825 830 VHDNFMNNEITASKIDDGNN GAL4 activation domain VHDNFMNNEITASKIDDGNN g t t c a t g a t a a c t t c a t g a a t a a t g a a a t c a c g g c t a g t a a a a t t g a t g a t g g t a a t a a t c a a g t a c t a t t g a a g t a c t t a t t a c t t t a g t g c c g a t c a t t t t a a c t a c t a c c a t t a t t a

MluISexAI*CsiIMmeI 480 835 840 845 850 SKPLSPGWTDQTAYNAFGIT GAL4 activation domain SKPLSPGWTDQTAYNAFGIT t c a a a a c c a c t g t c a c c t g g t t g g a c g g a c c a a a c t g c g t a t a a c g c g t t t g g a a t c a c t a g t t t t g g t g a c a g t g g a c c a a c c t g c c t g g t t t g a c g c a t a t t g c g c a a a c c t t a g t g a

MslIBstXI 540 855 860 865 870 TGMFNTTTMDDVYNYLFDDE GAL4 activation domain TGMFNTTTMDDVYNYLFDDE a c a g g g a t g t t t a a t a c c a c t a c a a t g g a t g a t g t a t a t a a c t a t c t a t t c g a t g a t g a a t g t c c c t a c a a a t t a t g g t g a t g t t a c c t a c t a c a t a t a t t g a t a g a t a a g c t a c t a c t t

BstYIBglIIMflI* 600 875 880 DTPPNPKKE (in frame with GAL4 activation domain) IFNTTHYRASA GAL4 activation domain T7 promoter T A A T A C G A C T C A C T A T A G G G T7 DTPPNPKKEIFNTTHYRASA g a t a c c c c a c c a a a c c c a a a a a a a g a g a t c t t t a a t a c g a c t c a c t a t a g g g c g a g c g c c c t a t g g g g t g g t t t g g g t t t t t t t c t c t a g a a a t t a t g c t g a g t g a t a t c c c g c t c g c g g

TaqIIPciINspIDrdIBstEIIEcoRI 660 (in frame with GAL4 activation domain) EFMNGAIGGDLLLNFPDMSV EFMNGAIGGDLLLNFPDMSV g a a t t c a t g a a t g g a g c t a t a g g a g g t g a c c t t t t g c t c a a t t t t c c t g a c a t g t c g g t c c t t a a g t a c t t a c c t c g a t a t c c t c c a c t g g a a a a c g a g t t a a a a g g a c t g t a c a g c c a g

NgoMIVNaeIBsp1286IBanII 720 (in frame with GAL4 activation domain) LERQRAHLKYLNPTFDSPLA LERQRAHLKYLNPTFDSPLA c t a g a g c g c c a a a g g g c t c a c c t c a a g t a c c t c a a t c c c a c c t t t g a t t c t c c t c t c g c c g a t c t c g c g g t t t c c c g a g t g g a g t t c a t g g a g t t a g g g t g g a a a c t a a g a g g a g a g c g g

780 (in frame with GAL4 activation domain) GFFADSSMITGGEMDSYLST GFFADSSMITGGEMDSYLST g g c t t c t t t g c c g a t t c t t c a a t g a t t a c c g g c g g c g a g a t g g a c a g c t a t c t t t c g a c t c c g a a g a a a c g g c t a a g a a g t t a c t a a t g g c c g c c g c t c t a c c t g t c g a t a g a a a g c t g a

End (818) BsmBIEsp3I 3ʹ 5ʹ 818 (in frame with GAL4 activation domain) AGLNLPMMYGET AGLNLPMMYGET g c c g g t t t g a a t c t t c c g a t g a t g t a c g g t g a g a c g a c c g g c c a a a c t t a g a a g g c t a c t a c a t g c c a c t c t g c t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.