Addgene VHACg/pDONR221 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: VHACg/pDONR221 Sequencing Result


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PciI (5705) DrdI (5603) pBR322ori-F (5550 .. 5569) ApaLI (5391) PflMI (4793) Bpu10I (4547) AsiSI - PvuI (4530) Kan-R (4172 .. 4191) pENTR-R (4094 .. 4113) M13 Reverse (3976 .. 3992) M13 rev T7 (3952 .. 3971) NcoI (3770) BstAPI (3608) SphI (3606) EcoRI (3285) PfoI * (3177) SnaBI (2821) L4440 (92 .. 109) BspQI - SapI (111) rrnB T2 terminator BsaHI (327) M13/pUC Forward (522 .. 544) M13 Forward (536 .. 553) M13 fwd AhdI (549) PspOMI (563) ApaI (567) PaeR7I - PspXI - XhoI (760) MfeI (981) XcmI (1017) NmeAIII (1474) BtgZI (1512) DraIII (1535) DraI (1558) CsiI - SexAI * (1722) HindIII (1965) PmlI (2298) BlpI (2421) AvrII (2665) PpuMI (2669) XmnI (2770) XbaI (2811) VHACg/pDONR221 5711 bp

Sequence View

5ʹ 3ʹ 60 c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t

BspQISapI 120 A G C G A G T C A G T G A G C G A G L4440 t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t

180 g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t

240 c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g

300 rrnB T2 terminator t a g c c a g g a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a a t c g g t c c t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t

BsaHI 360 g t t t g a t g c c t g g c a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c c a a a c t a c g g a c c g t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g

420 rrnB T1 terminator a c a a c g t t c a a a t c c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a t g t t g c a a g t t t a g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t

480 rrnB T1 terminator c a a c a g a t a a a a c g a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g t t g t c t a t t t t g c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c

540 M13 fwd C C C A G T C A C G A C G T T G T A A M13/pUC Forward T G T A A M13 Forward g c a g t t c c c t a c t c t c g c g t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a c g t c a a g g g a t g a g a g c g c a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t

PspOMIApaIAhdI 600 M13 fwd attL1 A A C G M13/pUC Forward A A C G A C G G C C A G T M13 Forward a a c g a c g g c c a g t c t t a a g c t c g g g c c c c a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c t t g c t g c c g g t c a g a a t t c g a g c c c g g g g t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g

660 attL1 c t g t t c g t t g c a a c a a a t t g a t g a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a g a c a a g c a a c g t t g t t t a a c t a c t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t

720 attL1 a g c a g g c t t t a g a t g a a t c a a t c t t c t t a c t t c a g a g a c t c g c g a t t t t g g t t t g a g g a c t c g t c c g a a a t c t a c t t a g t t a g a a g a a t g a a g t c t c t g a g c g c t a a a a c c a a a c t c c t g

PaeR7IPspXIXhoI 780 g a c g a t t c a g a g t t a g g g c t t t g a a g c t g a a t c a a a a c a c t c g a g c c a a t t t a g t t t g t a c t g c t a a g t c t c a a t c c c g a a a c t t c g a c t t a g t t t t g t g a g c t c g g t t a a a t c a a a c a t

840 a c c c t a g a a g t t t t t g t a g g g t t t t a c c g t c t c t g t t t t t t a t t a t t t g g a t c t c t g a a c t g g g a t c t t c a a a a a c a t c c c a a a a t g g c a g a g a c a a a a a a t a a t a a a c c t a g a g a c t t g

900 a c a t t t g g c c c a g t a t t a g c c c a t t t a c c t a t c t t c t c a a a a t a t t t g g t t c t t a a c a a a t g t a a a c c g g g t c a t a a t c g g g t a a a t g g a t a g a a g a g t t t t a t a a a c c a a g a a t t g t t t

960 t t c a a g c c t a a c c a t t a g t a c a a t t t t a a t t t t g a t c t g t a c a t t t t c a a a a a c c g g a c t a a g t t c g g a t t g g t a a t c a t g t t a a a a t t a a a a c t a g a c a t g t a a a a g t t t t t g g c c t g a

XcmIMfeI 1020 c g a a c c a a g a t c a a a t t c a a c a a t t g t g g c a a t t t t a a t g g t c g a g t c t c c a g t g t g t a t g c t t g g t t c t a g t t t a a g t t g t t a a c a c c g t t a a a a t t a c c a g c t c a g a g g t c a c a c a t a

1080 g t g g c a g t a g g a g a a c g t g c c g g g g g a t a t t a a a c c g g c t c a a t g c t a c a g t c g t a c c g g c a c c g t c a t c c t c t t g c a c g g c c c c c t a t a a t t t g g c c g a g t t a c g a t g t c a g c a t g g c c

1140 t t t g t t t t t t t a c t c g t t g g t a t a t a a t t a a a a c c g g t t a t c c a a t t t g a c a g g c a g a g a a a a c a a a a a a a t g a g c a a c c a t a t a t t a a t t t t g g c c a a t a g g t t a a a c t g t c c g t c t c t

1200 g a a g a a g a t t t g a t t g a a g t a c a g a t c c a t c g g a g a a g a a g a c a g a g a g a t t g a a g a t c a c t t c t t c t a a a c t a a c t t c a t g t c t a g g t a g c c t c t t c t t c t g t c t c t c t a a c t t c t a g t

1260 a t g a c t t c g a g a t a t t g g g t g g t g t c t c t t c c a g t g a a a g a c t c t g c t t c t t c g t t a t g g t a c t g a a g c t c t a t a a c c c a c c a c a g a g a a g g t c a c t t t c t g a g a c g a a g a a g c a a t a c c

1320 a a t c g a t t a c a a g a g c a g a t c t c t a a g c a t t c c t t t g a t a c t c c a g t t t a t c g g g t a c t c t t a g c t a a t g t t c t c g t c t a g a g a t t c g t a a g g a a a c t a t g a g g t c a a a t a g c c c a t g a g

1380 t g a t t t t t g c t c g a t t t g g t c t t a t t g c a a a a t t t a g c a c a g a t c t t a t a c t a t c t c t t g a c t a a a a a c g a g c t a a a c c a g a a t a a c g t t t t a a a t c g t g t c t a g a a t a t g a t a g a g a a c

1440 c t a c t t t c g t g t c t a c g a t t c t a a t t a c g a t c t a a t t c c g t g a g t t t t g g t t t c a a t t t c g a t g a a a g c a c a g a t g c t a a g a t t a a t g c t a g a t t a a g g c a c t c a a a a c c a a a g t t a a a g

NmeAIII 1500 g c a g t t t a a c a t t c c t a a t c t t c g t g t t g g a a c c t t a g a t t c t c t a c t c g c t c t c g g c g a c g t c a a a t t g t a a g g a t t a g a a g c a c a a c c t t g g a a t c t a a g a g a t g a g c g a g a g c c g c t

DraIDraIIIBtgZI 1560 t g a t c t g c t t a a g g t a a a a t t c a t t t g a t c a c t g a g t g g a t t t t a t g t t t t t g a t t t t a a a c t a g a c g a a t t c c a t t t t a a g t a a a c t a g t g a c t c a c c t a a a a t a c a a a a a c t a a a a t t

1620 a a t t c c t c t c t t g a t t g a g a a a t t t t t g a g a t t t g t a g t c g a a c a g c t t t g t g g a a g g a g t t a a g g a g a g a a c t a a c t c t t t a a a a a c t c t a a a c a t c a g c t t g t c g a a a c a c c t t c c t c

1680 t t t c g c a a a a g a t c a g g a g a c a g a t c g a a g a a t t g g a g a g g a t t t c t g g t g t t g a g a g c a a a a g c g t t t t c t a g t c c t c t g t c t a g c t t c t t a a c c t c t c c t a a a g a c c a c a a c t c t c g t

SexAI*CsiI 1740 a c g c t c t t a c a g t t g a t g g a g t t c c t g t t g a t t c t t a t c t c a c c a g g t a t t t g t g a t t t t t g c g a g a a t g t c a a c t a c c t c a a g g a c a a c t a a g a a t a g a g t g g t c c a t a a a c a c t a a a a

1800 c c g t c t c a g a a g g a g t t a a t t g c t a a t t t g g t g g t g a t c g a a t t g t t a a a g a a c g a a a c t g g c a g a g t c t t c c t c a a t t a a c g a t t a a a c c a c c a c t a g c t t a a c a a t t t c t t g c t t t g a

1860 t t t g t t g a t t g g t g g a c t c a a g g t t t g t g t g g g a t g a a g c t a a g t a c c c a a c a a t g t c a c a a a c a a c t a a c c a c c t g a g t t c c a a a c a c a c c c t a c t t c g a t t c a t g g g t t g t t a c a g t g

1920 c t c t g a a g g a g g t t g t g g a c a a t a t t c a g t c t c a g g t t g c a a a a a t t g a g g a t g a t c t c a g a g a c t t c c t c c a a c a c c t g t t a t a a g t c a g a g t c c a a c g t t t t t a a c t c c t a c t a g a g t

HindIII 1980 a g g t a t t g t a g c t t a a a c c t t t t g t t t t a t t g t a a a a a a a a a g g a a g c t t c a c c t t t a a g t c c a t a a c a t c g a a t t t g g a a a a c a a a a t a a c a t t t t t t t t t c c t t c g a a g t g g a a a t t c

2040 c a g a a a g a t t a g c t g c a t g a g a c c g a t g g t a t a a a a t t t g c a a t t c g a g t t t c t t a c g a t g t c t t t c t a a t c g a c g t a c t c t g g c t a c c a t a t t t t a a a c g t t a a g c t c a a a g a a t g c t a

2100 g a t c a a a t g a a t t g c t t t g g t t c a a a t g g t g g t g t t a a c g a c t c t g a t t g c a c a t t t t a t c t a g t t t a c t t a a c g a a a c c a a g t t t a c c a c c a c a a t t g c t g a g a c t a a c g t g t a a a a t a

2160 t c a g g t t c g t g t t g c t g a a t a t a a c a a t a t c c g t g g t c a a c t c a a t g c c a t t a a c c g a a a a g t c c a a g c a c a a c g a c t t a t a t t g t t a t a g g c a c c a g t t g a g t t a c g g t a a t t g g c t t t

2220 g c a a a g t g g a a g g t a a t t c t t t t c t t c t c a t a t t t a t a c t a t a a g a g c a c a c t a g a a a c t c g t t t c a c c t t c c a t t a a g a a a a g a a g a g t a t a a a t a t g a t a t t c t c g t g t g a t c t t t g a

2280 g t g g g c g g t t t a g c a a c a a c a a a t t t t g a a g t t t g t t t t c t c a t c t a t c t g a t t t t t g c t c a c c c g c c a a a t c g t t g t t g t t t a a a a c t t c a a a c a a a a g a g t a g a t a g a c t a a a a a c g a

PmlI 2340 t g a a g c a t g a t t t a c c a c g t g a a c g t t t t a g a a t a a t a c a a a g t t a t t t t c c a g a a t a t t a c t t c g t a c t a a a t g g t g c a c t t g c a a a a t c t t a t t a t g t t t c a a t a a a a g g t c t t a t a a

2400 g c t g t t g g t c t t t a t c g a t a t c t c a g c t t t g t t t a g t a t c t t t g t g g c a t t g t g g c t g a t c g a c a a c c a g a a a t a g c t a t a g a g t c g a a a c a a a t c a t a g a a a c a c c g t a a c a c c g a c t a

BlpI 2460 c g t c c t c c t t t t t g t c t c a g c t t a g c t g t t c g t g a c c t c t c a a a c t t g g t t a a g c c a g a a g c a g g a g g a a a a a c a g a g t c g a a t c g a c a a g c a c t g g a g a g t t t g a a c c a a t t c g g t c t t

2520 g a t a t t g t c g a g t c c g a a c a t c t c g t a a c t c t c c t t g c a g t t g t t c c g a a g t a t t c c c a a c t a t a a c a g c t c a g g c t t g t a g a g c a t t g a g a g g a a c g t c a a c a a g g c t t c a t a a g g g t t

2580 a a a g a c t g g c t a g c a t g t t a t g a g a c t t t g a c c g a t t a t g t g g t a a a t t t g t t g a c t t g c t t t c t g a c c g a t c g t a c a a t a c t c t g a a a c t g g c t a a t a c a c c a t t t a a a c a a c t g a a c g

2640 t a g g t t t a t t g a a t a g a c c a g t g g t a a c a c t a c t g g t t t g a c g t a c c g t g a t t t t a a t c a a t c c a a a t a a c t t a t c t g g t c a c c a t t g t g a t g a c c a a a c t g c a t g g c a c t a a a a t t a g t

AvrIIPpuMI 2700 a t t t a t c c t a a c t t g t t t c a g g t t c c t a g g t c c t c g a a g a a a t t g t t t g a g g a t a a t g a g t a a a t a g g a t t g a a c a a a g t c c a a g g a t c c a g g a g c t t c t t t a a c a a a c t c c t a t t a c t c

2760 t a t g c c c t t t a c a c c g t c a c t c t c t t t a c t c g t g t c g c a g a t a a t t t c a g g a t t g c t g c t a t a c g g g a a a t g t g g c a g t g a g a g a a a t g a g c a c a g c g t c t a t t a a a g t c c t a a c g a c g a

XbaIXmnI 2820 c g t g a g a a a g g a t t c c a a g t g a g t a t t c t g t t c a a g t t c c t g a t a t t a c c t c t a g a g t t a g c a c t c t t t c c t a a g g t t c a c t c a t a a g a c a a g t t c a a g g a c t a t a a t g g a g a t c t c a a t

SnaBI 2880 c g t a g a a g t t a a a a a t g a t t a c a t g c t a g t a g g a g t g a a a t t t c c t a c g a a c t g a t t g t a g c a t c t t c a a t t t t t a c t a a t g t a c g a t c a t c c t c a c t t t a a a g g a t g c t t g a c t a a c a t

2940 t t c a t g g t g c a a g t t c a g a a a t t t g g t t t a g a t t c a a a g a t t t g t t c a t c a t a t a t t g t a a a g t a c c a c g t t c a a g t c t t t a a a c c a a a t c t a a g t t t c t a a a c a a g t a g t a t a t a a c a t

3000 c a c c a t c c t c t t c t a g g t c c g t g a t t t t g a a c a a a g t g t t g a a g c a c a a g a g a c t c g t a a g t g g t a g g a g a a g a t c c a g g c a c t a a a a c t t g t t t c a c a a c t t c g t g t t c t c t g a g c a t t

3060 a c a a g a g c t a g c a a a g t t g g t t c a g g a t c a g g a a a g t t t g a g a a g c t c t c t t t t g c a g t g t g t t c t c g a t c g t t t c a a c c a a g t c c t a g t c c t t t c a a a c t c t t c g a g a g a a a a c g t c a c

3120 g t g c t a c a c c a g t t a t g g a g a g g t a g g t a t c t a c a c a t t g c t c t g a a t a g a t a t t c t c t t c a c g a t g t g g t c a a t a c c t c t c c a t c c a t a g a t g t g t a a c g a g a c t t a t c t a t a a g a g a a

PfoI* 3180 g t a a t a c a a t a c c t g a a t c t g t t t t g a t c t t c g g t c t a c a t c t g t a g g t t t t c a g c t c c t c a t t a t g t t a t g g a c t t a g a c a a a a c t a g a a g c c a g a t g t a g a c a t c c a a a a g t c g a g g a

3240 g g a t g c a t t t c t g t g c t g t g c g t a c a t t c g c t g a g a g c a t t a t g a g a t a c g g t t t a c c t c c c t a c g t a a a g a c a c g a c a c g c a t g t a a g c g a c t c t c g t a a t a c t c t a t g c c a a a t g g a g

EcoRI 3300 c g g c g t t c t t g g t a a a t t a g g a c t c c a a g c t a t g c t a t t t t a g a g a a t t c c t c t a c c c t a g c c g c a a g a a c c a t t t a a t c c t g a g g t t c g a t a c g a t a a a a t c t c t t a a g g a g a t g g g a t

3360 c t a t c t c c c t t a c c a a a t t a c t t t c t c t t g t c t t t c c a g g c a t g t g t c t t a t c t c c g g c t g a t a g a g g g a a t g g t t t a a t g a a a g a g a a c a g a a a g g t c c g t a c a c a g a a t a g a g g c c g a

3420 g t g a a a a g t g a a a a g a a a g t a c g c t c c a t t c t t g a a c g c t t g t g t g a t t c t a c c a a c a g g c a c t t t t c a c t t t t c t t t c a t g c g a g g t a a g a a c t t g c g a a c a c a c t a a g a t g g t t g t c c

3480 t a t g t t a a a a c t c t c t a c c t a t c t a t c g c t c t a g g t g t a g g a t a c a t g g a a g c t g t t t a a a t a c a a t t t t g a g a g a t g g a t a g a t a g c g a g a t c c a c a t c c t a t g t a c c t t c g a c a a a t t

3540 c a t c a a c c t t c a t a t t g a t c t g g c g a a g a a a a a t g t a c t g a t a g t t a t g a c c t t a a t a a c g t a g t t g g a a g t a t a a c t a g a c c g c t t c t t t t t a c a t g a c t a t c a a t a c t g g a a t t a t t g

3600 g a a a g a a a t t g c a g a a g t t t g a g g a g a a a g t c a g g a a c c a a g t a a a a c c a a a c t t g t a t t c t t t c t t t a a c g t c t t c a a a c t c c t c t t t c a g t c c t t g g t t c a t t t t g g t t t g a a c a t a a

SphIBstAPI 3660 a g c a t g c a a t g c g t t c t t g a g g c t a a a t c t a t t a a t c a g a a c t a a g t c g c t t t a g t t a g a t c g t a c g t t a c g c a a g a a c t c c g a t t t a g a t a a t t a g t c t t g a t t c a g c g a a a t c a a t c t

3720 t t c a t c t a t t g a t g t a g t g t a g t t c g a t g a g a t t t c a a a t t g a g c g t t t t g g g t c t c a t t a a g t a g a t a a c t a c a t c a c a t c a a g c t a c t c t a a a g t t t a a c t c g c a a a a c c c a g a g t a a

NcoI 3780 g t a t g a a a a c a a t g c a g t t t a t a c t g g a a a a g c g a g g a g g a t g c a g g a g c c a t g g c t g g t c a t a c t t t t g t t a c g t c a a a t a t g a c c t t t t c g c t c c t c c t a c g t c c t c g g t a c c g a c c a

3840 attL2 t t a g c t g g t g a c t c a g a g a c a c a t c c t t a t g t c t c c t t c a c t a t c a a c c t t g c t c a c c c a a a t c g a c c a c t g a g t c t c t g t g t a g g a a t a c a g a g g a a g t g a t a g t t g g a a c g a g t g g g t

3900 attL2 g c t t t c t t g t a c a a a g t t g g c a t t a t a a g a a a g c a t t g c t t a t c a a t t t g t t g c a a c g a a c g a a a g a a c a t g t t t c a a c c g t a a t a t t c t t t c g t a a c g a a t a g t t a a a c a a c g t t g c t t

3960 attL2 T7 promoter G G G A T A T C A T7 c a g g t c a c t a t c a g t c a a a a t a a a a t c a t t a t t t g c c a t c c a g c t g a t a t c c c c t a t a g t g t c c a g t g a t a g t c a g t t t t a t t t t a g t a a t a a a c g g t a g g t c g a c t a t a g g g g a t a t c a

4020 T7 promoter M13 rev C T C A G C A T A A T T7 C A G T A T C G A C A A A G G A C M13 Reverse g a g t c g t a t t a c a t g g t c a t a g c t g t t t c c t g g c a g c t c t g g c c c g t g t c t c a a a a t c t c c t c a g c a t a a t g t a c c a g t a t c g a c a a a g g a c c g t c g a g a c c g g g c a c a g a g t t t t a g a g

4080 t g a t g t t a c a t t g c a c a a g a t a a a a a t a t a t c a t c a t g a a c a a t a a a a c t g t c t g c t t a c a c t a c a a t g t a a c g t g t t c t a t t t t t a t a t a g t a g t a c t t g t t a t t t t g a c a g a c g a a t g

4140 1 5 10 MSHIQRETSRPR KanR G T T C C C C A C A A T A C T C G G T A pENTR-R MSHIQRETSRPR a t a a a c a g t a a t a c a a g g g g t g t t a t g a g c c a t a t t c a a c g g g a a a c g t c g a g g c c g c g a t a t t t g t c a t t a t g t t c c c c a c a a t a c t c g g t a t a a g t t g c c c t t t g c a g c t c c g g c g c t

4200 15 20 25 30 LNSNMDADLYGYKWARDNVG KanR C C A T A T T T A C C C G A G C G C T A Kan-R LNSNMDADLYGYKWARDNVG t t a a a t t c c a a c a t g g a t g c t g a t t t a t a t g g g t a t a a a t g g g c t c g c g a t a a t g t c g g g a a t t t a a g g t t g t a c c t a c g a c t a a a t a t a c c c a t a t t t a c c c g a g c g c t a t t a c a g c c c

4260 35 40 45 50 QSGATIYRLYGKPDAPELFL KanR QSGATIYRLYGKPDAPELFL c a a t c a g g t g c g a c a a t c t a t c g c t t g t a t g g g a a g c c c g a t g c g c c a g a g t t g t t t c t g g t t a g t c c a c g c t g t t a g a t a g c g a a c a t a c c c t t c g g g c t a c g c g g t c t c a a c a a a g a c

4320 55 60 65 70 KHGKGSVANDVTDEMVRLNW KanR KHGKGSVANDVTDEMVRLNW a a a c a t g g c a a a g g t a g c g t t g c c a a t g a t g t t a c a g a t g a g a t g g t c a g a c t a a a c t g g t t t g t a c c g t t t c c a t c g c a a c g g t t a c t a c a a t g t c t a c t c t a c c a g t c t g a t t t g a c c

4380 75 80 85 90 LTEFMPLPTIKHFIRTPDDA KanR LTEFMPLPTIKHFIRTPDDA c t g a c g g a a t t t a t g c c t c t t c c g a c c a t c a a g c a t t t t a t c c g t a c t c c t g a t g a t g c a g a c t g c c t t a a a t a c g g a g a a g g c t g g t a g t t c g t a a a a t a g g c a t g a g g a c t a c t a c g t

4440 95 100 105 110 WLLTTAIPGKTAFQVLEEYP KanR WLLTTAIPGKTAFQVLEEYP t g g t t a c t c a c c a c t g c g a t c c c c g g a a a a a c a g c a t t c c a g g t a t t a g a a g a a t a t c c t a c c a a t g a g t g g t g a c g c t a g g g g c c t t t t t g t c g t a a g g t c c a t a a t c t t c t t a t a g g a

4500 115 120 125 130 DSGENIVDALAVFLRRLHSI KanR DSGENIVDALAVFLRRLHSI g a t t c a g g t g a a a a t a t t g t t g a t g c g c t g g c a g t g t t c c t g c g c c g g t t g c a t t c g a t t c t a a g t c c a c t t t t a t a a c a a c t a c g c g a c c g t c a c a a g g a c g c g g c c a a c g t a a g c t a a

Bpu10IAsiSIPvuI 4560 135 140 145 150 PVCNCPFNSDRVFRLAQAQS KanR PVCNCPFNSDRVFRLAQAQS c c t g t t t g t a a t t g t c c t t t t a a c a g c g a t c g c g t a t t t c g t c t c g c t c a g g c g c a a t c a g g a c a a a c a t t a a c a g g a a a a t t g t c g c t a g c g c a t a a a g c a g a g c g a g t c c g c g t t a g t

4620 155 160 165 170 RMNNGLVDASDFDDERNGWP KanR RMNNGLVDASDFDDERNGWP c g a a t g a a t a a c g g t t t g g t t g a t g c g a g t g a t t t t g a t g a c g a g c g t a a t g g c t g g c c t g c t t a c t t a t t g c c a a a c c a a c t a c g c t c a c t a a a a c t a c t g c t c g c a t t a c c g a c c g g a

4680 175 180 185 190 VEQVWKEMHKLLPFSPDSVV KanR VEQVWKEMHKLLPFSPDSVV g t t g a a c a a g t c t g g a a a g a a a t g c a t a a a c t t t t g c c a t t c t c a c c g g a t t c a g t c g t c c a a c t t g t t c a g a c c t t t c t t t a c g t a t t t g a a a a c g g t a a g a g t g g c c t a a g t c a g c a g

4740 195 200 205 210 THGDFSLDNLIFDEGKLIGC KanR THGDFSLDNLIFDEGKLIGC a c t c a t g g t g a t t t c t c a c t t g a t a a c c t t a t t t t t g a c g a g g g g a a a t t a a t a g g t t g t t g a g t a c c a c t a a a g a g t g a a c t a t t g g a a t a a a a a c t g c t c c c c t t t a a t t a t c c a a c a

PflMI 4800 215 220 225 230 IDVGRVGIADRYQDLAILWN KanR IDVGRVGIADRYQDLAILWN a t t g a t g t t g g a c g a g t c g g a a t c g c a g a c c g a t a c c a g g a t c t t g c c a t c c t a t g g a a c t a a c t a c a a c c t g c t c a g c c t t a g c g t c t g g c t a t g g t c c t a g a a c g g t a g g a t a c c t t g

4860 235 240 245 250 CLGEFSPSLQKRLFQKYGID KanR CLGEFSPSLQKRLFQKYGID t g c c t c g g t g a g t t t t c t c c t t c a t t a c a g a a a c g g c t t t t t c a a a a a t a t g g t a t t g a t a c g g a g c c a c t c a a a a g a g g a a g t a a t g t c t t t g c c g a a a a a g t t t t t a t a c c a t a a c t a

4920 255 260 265 270 NPDMNKLQFHLMLDEFF* KanR NPDMNKLQFHLMLDEFF* a a t c c t g a t a t g a a t a a a t t g c a g t t t c a t t t g a t g c t c g a t g a g t t t t t c t a a t c a g a a t t a g g a c t a t a c t t a t t t a a c g t c a a a g t a a a c t a c g a g c t a c t c a a a a a g a t t a g t c t t

4980 t t g g t t a a t t g g t t g t a a c a c t g g c a g a g c a t t a c g c t g a c t t g a c g g g a c g g c g c a a g c a a c c a a t t a a c c a a c a t t g t g a c c g t c t c g t a a t g c g a c t g a a c t g c c c t g c c g c g t t c g

5040 t c a t g a c c a a a a t c c c t t a a c g t g a g t t a c g c g t c g t t c c a c t g a g c g t c a g a c c c c g t a a g t a c t g g t t t t a g g g a a t t g c a c t c a a t g c g c a g c a a g g t g a c t c g c a g t c t g g g g c a t

5100 ori g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t

5160 ori a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a

5220 ori t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c

5280 ori c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t

5340 ori a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t

ApaLI 5400 ori a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c

5460 ori c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t

5520 ori a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t

5580 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g

DrdI 5640 ori g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g

5700 ori c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a

PciI 3ʹ 5ʹ 5711 g c t c a c a t g t t c g a g t g t a c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.