Addgene pAAV.hSynap.iAChSnFR-Venus-NULL - Sequence Analyzer Skip to main content
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Sequence Analyzer: pAAV.hSynap.iAChSnFR-Venus-NULL


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BspQI - SapI (6191) NspI (6078) AflIII - PciI (6074) pBR322ori-F (5919 .. 5938) BsaI (5120) ScaI (4705) Amp-R (4617 .. 4636) XmnI (4586) F1ori-F (4110 .. 4131) F1ori-R (3900 .. 3919) M13/pUC Forward (3664 .. 3686) M13 Forward (3655 .. 3672) M13 fwd PacI (3643) MfeI (3354) SV40pA-R (3289 .. 3308) EBV-rev (3235 .. 3254) EagI - NotI (3202) pBluescriptKS (3179 .. 3195) KS primer FspAI (422) BbvCI (489) RsrII (585) KasI (642) NarI (643) SfoI (644) MreI - SgrAI (645) PluTI (646) AfeI (658) EcoRI (716) NheI (727) BmtI (731) BamHI (735) NcoI (746) AflII (910) BlpI (1228) EGFP-C (1260 .. 1281) Acc65I (1327) KpnI (1331) EGFP-N (1384 .. 1405) PasI (1530) BstEII (2008) StuI * (2018) BstXI (2061) BmgBI (2253) NruI (2269) PpuMI (2331) BfuAI - BspMI (2373) Myc XcmI (2505) HindIII (2575) WPRE-R (2640 .. 2660) BbsI (3115) pAAV.hSynap.iAChSnFR-Venus-NULL 6200 bp

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60 AAV2 ITR g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g c g c c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c g c g

120 AAV2 ITR g c t c g c t c g c t c a c t g a g g c c g c c c g g g c a a a g c c c g g g c g t c g g g c g a c c t t t g g t c g c c g a g c g a g c g a g t g a c t c c g g c g g g c c c g t t t c g g g c c c g c a g c c c g c t g g a a a c c a g c g

180 AAV2 ITR c c g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a a c t c c a t c a c t a g g g g t g g c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t t g a g g t a g t g a t c c c c a

240 AAV2 ITR t c c t t g t a g t t a a t g a t t a a c c c g c c a t g c t a c t t a t c t a c g t a g c c a t g c t c t a g g a a g a g g a a c a t c a a t t a c t a a t t g g g c g g t a c g a t g a a t a g a t g c a t c g g t a c g a g a t c c t t c

300 hSyn promoter a t c t c t g c a g a g g g c c c t g c g t a t g a g t g c a a g t g g g t t t t a g g a c c a g g a t g a g g c g g g t a g a g a c g t c t c c c g g g a c g c a t a c t c a c g t t c a c c c a a a a t c c t g g t c c t a c t c c g c c c

360 hSyn promoter g t g g g g g t g c c t a c c t g a c g a c c g a c c c c g a c c c a c t g g a c a a g c a c c c a a c c c c c a t t c c a c c c c c a c g g a t g g a c t g c t g g c t g g g g c t g g g t g a c c t g t t c g t g g g t t g g g g g t a a g

420 hSyn promoter c c c a a a t t g c g c a t c c c c t a t c a g a g a g g g g g a g g g g a a a c a g g a t g c g g c g a g g c g c g t g g g t t t a a c g c g t a g g g g a t a g t c t c t c c c c c t c c c c t t t g t c c t a c g c c g c t c c g c g c a

FspAI 480 hSyn promoter g c g c a c t g c c a g c t t c a g c a c c g c g g a c a g t g c c t t c g c c c c c g c c t g g c g g c g c g c g c c c g c g t g a c g g t c g a a g t c g t g g c g c c t g t c a c g g a a g c g g g g g c g g a c c g c c g c g c g c g g

BbvCI 540 hSyn promoter a c c g c c g c c t c a g c a c t g a a g g c g c g c t g a c g t c a c t c g c c g g t c c c c c g c a a a c t c c c c t g g c g g c g g a g t c g t g a c t t c c g c g c g a c t g c a g t g a g c g g c c a g g g g g c g t t t g a g g g g

RsrII 600 hSyn promoter t t c c c g g c c a c c t t g g t c g c g t c c g c g c c g c c g c c g g c c c a g c c g g a c c g c a c c a c g c g a a a g g g c c g g t g g a a c c a g c g c a g g c g c g g c g g c g g c c g g g t c g g c c t g g c g t g g t g c g c t

AfeIKasINarISfoISgrAIMreIPluTI 660 hSyn promoter g g c g c g a g a t a g g g g g g c a c g g g c g c g a c c a t c t g c g c t g c g g c g c c g g c g a c t c a g c g c c c g c g c t c t a t c c c c c c g t g c c c g c g c t g g t a g a c g c g a c g c c g c g g c c g c t g a g t c g c g

EcoRI 720 hSyn promoter t g c c t c a g t c t g c g g t g g g c a g c g g a g g a g t c g t g t c g t g c c t g a g a g c g c a g t c g a a t t a c g g a g t c a g a c g c c a c c c g t c g c c t c c t c a g c a c a g c a c g g a c t c t c g c g t c a g c t t a a

NcoIBamHIBmtINheI 780 1 5 10 METDTLLLWVL Ig κ leader Kozak sequence METDTLLLWVL c a a g c t g c t a g c a a g g a t c c c g c c a c c a t g g a g a c a g a c a c a c t c c t g c t a t g g g t a c t g g t t c g a c g a t c g t t c c t a g g g c g g t g g t a c c t c t g t c t g t g t g a g g a c g a t a c c c a t g a c

840 15 20 LLWVPGSTGD (in frame with Ig κ leader) RSANDTVVVG Ig κ leader LLWVPGSTGDRSANDTVVVG c t g c t c t g g g t t c c a g g t t c c a c t g g t g a c a g a t c t g c g a a c g a c a c c g t a g t t g t g g g c g a c g a g a c c c a a g g t c c a a g g t g a c c a c t g t c t a g a c g c t t g c t g t g g c a t c a a c a c c c g

900 (in frame with Ig κ leader) SIIFTEGIIVANMVAEMIEA SIIFTEGIIVANMVAEMIEA t c g a t c a t c t t t a c a g a a g g g a t t a t c g t c g c a a a c a t g g t g g c a g a g a t g a t t g a g g c g a g c t a g t a g a a a t g t c t t c c c t a a t a g c a g c g t t t g t a c c a c c g t c t c t a c t a a c t c c g c

AflII 960 (in frame with Ig κ leader) HTDLKVVRKLNLGGVNVNFE HTDLKVVRKLNLGGVNVNFE c a t a c a g a c c t t a a g g t g g t t c g c a a a c t g a a c c t t g g c g g g g t c a a c g t t a a c t t t g a a g t a t g t c t g g a a t t c c a c c a a g c g t t t g a c t t g g a a c c g c c c c a g t t g c a a t t g a a a c t t

1020 (in frame with Ig κ leader) AIKRGGANNGIDIYVEYTGH AIKRGGANNGIDIYVEYTGH g c c a t t a a a c g c g g a g g t g c g a a t a a t g g t a t t g a c a t t t a c g t g g a g t a c a c t g g g c a c c g g t a a t t t g c g c c t c c a c g c t t a t t a c c a t a a c t g t a a a t g c a c c t c a t g t g a c c c g t g

1080 (in frame with Ig κ leader) GLVDILGFPEPNVYITADKQ GLVDILGFPEPNVYITADKQ g g t c t t g t g g a t a t t c t g g g g t t c c c g g a g c c g a a c g t c t a t a t c a c c g c c g a c a a g c a g c c a g a a c a c c t a t a a g a c c c c a a g g g c c t c g g c t t g c a g a t a t a g t g g c g g c t g t t c g t c

1140 (in frame with Ig κ leader) KNGIKANFKIRHNVEDGSVQ KNGIKANFKIRHNVEDGSVQ a a g a a c g g c a t c a a g g c g a a c t t c a a g a t c c g c c a c a a c g t g g a g g a c g g c a g c g t g c a g t t c t t g c c g t a g t t c c g c t t g a a g t t c t a g g c g g t g t t g c a c c t c c t g c c g t c g c a c g t c

1200 (in frame with Ig κ leader) LADHYQQNTPIGDGPVLLPD LADHYQQNTPIGDGPVLLPD c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g

BlpI 1260 (in frame with Ig κ leader) NHYLSYQSVLSKDPNEKRDH C EGFP-C NHYLSYQSVLSKDPNEKRDH a a c c a c t a c c t g a g c t a t c a g t c c g t g c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c t t g g t g a t g g a c t c g a t a g t c a g g c a c g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g

1320 (in frame with Ig κ leader) MVLLEFVTAAGITLGMNELY A T G G T C C T G C T G G A G T T C G T G EGFP-C MVLLEFVTAAGITLGMNELY a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g a a c g a g c t g t a c t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c t t g c t c g a c a t g

Acc65IKpnI 1380 (in frame with Ig κ leader) KGGTGGSMSKGEELFTGVVP KGGTGGSMSKGEELFTGVVP a a g g g c g g t a c c g g a g g g a g c a t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c t t c c c g c c a t g g c c t c c c t c g t a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g

1440 (in frame with Ig κ leader) ILVELDGDVNGHKFSVRGEG G A C C A G C T C G A C C T G C C G C T G C EGFP-N ILVELDGDVNGHKFSVRGEG a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g c g c g g c g a g g g c t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c g c g c c g c t c c c g

1500 (in frame with Ig κ leader) EGDATNGKLTLKLLCTTGKL EGDATNGKLTLKLLCTTGKL g a g g g c g a t g c c a c c a a c g g c a a g c t g a c c c t g a a g c t c c t g t g c a c c a c c g g c a a g c t g c t c c c g c t a c g g t g g t t g c c g t t c g a c t g g g a c t t c g a g g a c a c g t g g t g g c c g t t c g a c

PasI 1560 (in frame with Ig κ leader) PVPWPTLVTTLGYGVQCFAR PVPWPTLVTTLGYGVQCFAR c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g g g c t a c g g c g t g c a g t g c t t c g c c c g c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c c c g a t g c c g c a c g t c a c g a a g c g g g c g

1620 (in frame with Ig κ leader) YPDHMKQHDFFKSAMPEGYV YPDHMKQHDFFKSAMPEGYV t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g

1680 (in frame with Ig κ leader) QERTISFKDDGTYKTRAEVK QERTISFKDDGTYKTRAEVK c a g g a g c g c a c c a t c a g c t t c a a g g a c g a c g g c a c c t a c a a g a c c c g c g c c g a g g t g a a g g t c c t c g c g t g g t a g t c g a a g t t c c t g c t g c c g t g g a t g t t c t g g g c g c g g c t c c a c t t c

1740 (in frame with Ig κ leader) FEGDTLVNRIELKGIDFKED FEGDTLVNRIELKGIDFKED t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g

1800 (in frame with Ig κ leader) GNILGHKLEYNFPPPATTDP GNILGHKLEYNFPPPATTDP g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t t t c c g c c g c c c g c g a c t a c t g a t c c a c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a a a g g c g g c g g g c g c t g a t g a c t a g g t

1860 (in frame with Ig κ leader) EGAYETVKKEYKRKWNIVWL EGAYETVKKEYKRKWNIVWL g a a g g t g c a t a c g a a a c c g t g a a g a a g g a g t a c a a a c g t a a a t g g a a t a t t g t a t g g c t c c t t c c a c g t a t g c t t t g g c a c t t c t t c c t c a t g t t t g c a t t t a c c t t a t a a c a t a c c g a g

1920 (in frame with Ig κ leader) KPLGFNNTATLTVKDELAKQ KPLGFNNTATLTVKDELAKQ a a a c c a c t g g g a t t c a a c a a t a c g g c g a c g c t t a c c g t t a a a g a c g a a c t g g c g a a a c a g t t t g g t g a c c c t a a g t t g t t a t g c c g c t g c g a a t g g c a a t t t c t g c t t g a c c g c t t t g t c

1980 (in frame with Ig κ leader) YNLKTFSDLAKISDKLILGA YNLKTFSDLAKISDKLILGA t a t a a c c t t a a a a c c t t c a g t g a c t t a g c g a a a a t c t c g g a t a a g c t g a t t c t g g g t g c a a t a t t g g a a t t t t g g a a g t c a c t g a a t c g c t t t t a g a g c c t a t t c g a c t a a g a c c c a c g t

StuI*BstEII 2040 (in frame with Ig κ leader) TMFFLEGPDGYPGLQKLYNF TMFFLEGPDGYPGLQKLYNF a c g a t g t t c t t t t t a g a a g g g c c c g a t g g t t a c c c a g g c c t g c a a a a a c t g t a c a a t t t c t g c t a c a a g a a a a a t c t t c c c g g g c t a c c a a t g g g t c c g g a c g t t t t t g a c a t g t t a a a g

BstXI 2100 (in frame with Ig κ leader) KFKHTKSMDMGIRYTAIDNN KFKHTKSMDMGIRYTAIDNN a a a t t c a a g c a c a c c a a a a g c a t g g a c a t g g g t a t t c g c t a t a c c g c c a t t g a t a a t a a c t t t a a g t t c g t g t g g t t t t c g t a c c t g t a c c c a t a a g c g a t a t g g c g g t a a c t a t t a t t g

2160 (in frame with Ig κ leader) EVQVIDAWATDGLLVSHKLK EVQVIDAWATDGLLVSHKLK g a a g t t c a g g t a a t t g a t g c c t g g g c c a c t g a t g g c t t g c t g g t g a g c c a c a a a t t a a a a c t t c a a g t c c a t t a a c t a c g g a c c c g g t g a c t a c c g a a c g a c c a c t c g g t g t t t a a t t t t

2220 (in frame with Ig κ leader) ILEDDKAFFPPYYAAPIIRQ ILEDDKAFFPPYYAAPIIRQ a t t c t g g a g g a t g a t a a a g c g t t c t t c c c g c c g t a t t a t g c t g c c c c c a t c a t c c g t c a g t a a g a c c t c c t a c t a t t t c g c a a g a a g g g c g g c a t a a t a c g a c g g g g g t a g t a g g c a g t c

NruIBmgBI 2280 (in frame with Ig κ leader) DVLDKHPELKDVLNKLANQI DVLDKHPELKDVLNKLANQI g a t g t c t t a g a t a a g c a t c c t g a a c t g a a g g a c g t g c t g a a c a a a c t c g c g a a t c a a a t t c t a c a g a a t c t a t t c g t a g g a c t t g a c t t c c t g c a c g a c t t g t t t g a g c g c t t a g t t t a a

PpuMI 2340 (in frame with Ig κ leader) SLEEMQKLNYKVDGEGQDPA SLEEMQKLNYKVDGEGQDPA t c a c t g g a a g a a a t g c a g a a a c t g a a t t a c a a g g t g g a c g g t g a g g g t c a g g a c c c a g c g a g t g a c c t t c t t t a c g t c t t t g a c t t a a t g t t c c a c c t g c c a c t c c c a g t c c t g g g t c g c

BspMIBfuAI 2400 (in frame with Ig κ leader) KVAKEFLKEKGLILQVD 1 EQK Myc KVAKEFLKEKGLILQVDEQK a a a g t a g c t a a g g a g t t t t t g a a a g a g a a a g g t t t a a t t c t g c a g g t c g a c g a a c a a a a a t t t c a t c g a t t c c t c a a a a a c t t t c t c t t t c c a a a t t a a g a c g t c c a g c t g c t t g t t t t t

2460 5 10 LISEEDL N 1 5 10 AVGQDTQEVIVV Myc PDGFR β TM domain LISEEDLNAVGQDTQEVIVV c t c a t c t c a g a a g a g g a t c t g a a t g c t g t g g g c c a g g a c a c g c a g g a g g t c a t c g t g g t g g a g t a g a g t c t t c t c c t a g a c t t a c g a c a c c c g g t c c t g t g c g t c c t c c a g t a g c a c c a c

XcmI 2520 15 20 25 30 PHSLPFKVVVISAILALVVL PDGFR β TM domain PHSLPFKVVVISAILALVVL c c a c a c t c c t t g c c c t t t a a g g t g g t g g t g a t c t c a g c c a t c c t g g c c c t g g t g g t g c t c g g t g t g a g g a a c g g g a a a t t c c a c c a c c a c t a g a g t c g g t a g g a c c g g g a c c a c c a c g a g

HindIII 2580 35 40 45 TIISLIILIMLWQKKPR * PDGFR β TM domain TIISLIILIMLWQKKPR* a c c a t c a t c t c c c t t a t c a t c c t c a t c a t g c t t t g g c a g a a g a a g c c a c g t t a g a a g c t t t g g t a g t a g a g g g a a t a g t a g g a g t a g t a c g a a a c c g t c t t c t t c g g t g c a a t c t t c g a a

2640 WPRE A WPRE-R a t c g a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t t a g c t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a

2700 WPRE C A A C G A G G A A A A T G C G A T A C WPRE-R MPLYHAIA MLLL g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a

2760 WPRE SRMAFIFSSLYKSWLLSLYE PVWLSFSPPCINPGCCLFMR t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c

2820 WPRE ELWPVVRQRGVVCTVFADAT SCGPLSGNVAWCALCLLTQP g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g

2880 WPRE PTGWGIATTCQLLSGTFAFP PLVGALPPPVSSFPGLSLSP c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g

2940 WPRE LPIATAELIAACLARCWTGA SLLPRRNSSPPALPAAGQGL c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a

3000 WPRE (in frame with Factor Xa site) *RGKRP RLLGTDNSVVLSGKSSSFPW GCWALTIPWCCRGNHRPFLG c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c

3060 WPRE (in frame with Factor Xa site) QEGTNGGPNQAPRGEAVDR R Factor Xa site LLACVATWILRGTSFCYVPS CSPVLPPGFCAGRPSATSLR c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c

BbsI 3120 WPRE 1 GEI Factor Xa site ALNPADLPSRGLLPALRPLP PSIQRTFLPAACCRLCGLFR g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c

3180 WPRE KS primer C T pBluescriptKS RLRLRPQTSRISLWAASPHR VFAFALRRVGSPFGPPPRID c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g a g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c t

EagINotI 3240 KS primer SV40 poly(A) signal A T G G C A G C T G G A G C T pBluescriptKS C T A C T C EBV-rev YRRPRPGRPLRADMIRYIDE TVDLDPGGRFEQT* t a c c g t c g a c c t c g a c c c g g g c g g c c g c t t c g a g c a g a c a t g a t a a g a t a c a t t g a t g a g a t g g c a g c t g g a g c t g g g c c c g c c g g c g a a g c t c g t c t g t a c t a t t c t a t g t a a c t a c t c

3300 SV40 poly(A) signal G A A A T T T G T G A T SV40pA-R A A A C C T G T T T G G T G EBV-rev FGQTTTRMQ* t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a

MfeI 3360 SV40 poly(A) signal G C T A T T G C SV40pA-R g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g

3420 a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g a t g t g g g a g g t t t t t t a a a g c a a g t a a a a c t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c t a c a c c c t c c a a a a a a t t t c g t t c a t t t t g

3480 c t c t a c a a a t g t g g t a a a a t c g a t a a g g a t c t t c c t a g a g c a t g g c t a c g t a g a t a a g t a g a g a t g t t t a c a c c a t t t t a g c t a t t c c t a g a a g g a t c t c g t a c c g a t g c a t c t a t t c a t

3540 AAV2 ITR g c a t g g c g g g t t a a t c a t t a a c t a c a a g g a a c c c c t a g t g a t g g a g t t g g c c a c t c c c t c c g t a c c g c c c a a t t a g t a a t t g a t g t t c c t t g g g g a t c a c t a c c t c a a c c g g t g a g g g a g

3600 AAV2 ITR t c t g c g c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c c g a c g c c c g g g c t t a g a c g c g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g g c t g c g g g c c c g a a

PacI 3660 AAV2 ITR M13 fwd T G A C C G M13 Forward t g c c c g g g c g g c c t c a g t g a g c g a g c g a g c g c g c a g c c t t a a t t a a c c t a a t t c a c t g g c a c g g g c c c g c c g g a g t c a c t c g c t c g c t c g c g c g t c g g a a t t a a t t g g a t t a a g t g a c c g

3720 M13 fwd G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g

3780 a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g

3840 f1 ori c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g

3900 f1 ori G F1ori-R g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g

3960 f1 ori A G G A A A G C G A A A G A A G G G A F1ori-R t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a

4020 f1 ori a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t

4080 f1 ori a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g

4140 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a

4200 f1 ori c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c

4260 f1 ori g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a

4320 f1 ori AmpR promoter t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g

4380 AmpR promoter t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t

4440 1 5 10 signal sequence MSIQHFRVALIPFF AmpR promoter AmpR MSIQHFRVALIPFF t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a

4500 15 20 signal sequence AAFCLPVFA 25 30 HPETLVKVKDA AmpR AAFCLPVFAHPETLVKVKDA g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a

4560 35 40 45 50 EDQLGARVGYIELDLNSGKI AmpR EDQLGARVGYIELDLNSGKI g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g

XmnI 4620 55 60 65 70 LESFRPEERFPMMSTFKVLL AmpR C G A T Amp-R LESFRPEERFPMMSTFKVLL c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t

4680 75 80 85 90 CGAVLSRIDAGQEQLGRRIH AmpR A C A C C G C G C C A T A A T A Amp-R CGAVLSRIDAGQEQLGRRIH t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g

ScaI 4740 95 100 105 110 YSQNDLVEYSPVTEKHLTDG AmpR YSQNDLVEYSPVTEKHLTDG t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g

4800 115 120 125 130 MTVRELCSAAITMSDNTAAN AmpR MTVRELCSAAITMSDNTAAN a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g

4860 135 140 145 150 LLLTTIGGPKELTAFLHNMG AmpR LLLTTIGGPKELTAFLHNMG t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c

4920 155 160 165 170 DHVTRLDRWEPELNEAIPND AmpR DHVTRLDRWEPELNEAIPND g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g

4980 175 180 185 190 ERDTTMPVAMATTLRKLLTG AmpR ERDTTMPVAMATTLRKLLTG g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g

5040 195 200 205 210 ELLTLASRQQLIDWMEADKV AmpR ELLTLASRQQLIDWMEADKV g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a

5100 215 220 225 230 AGPLLRSALPAGWFIADKSG AmpR AGPLLRSALPAGWFIADKSG g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t

BsaI 5160 235 240 245 250 AGERGSRGIIAALGPDGKPS AmpR AGERGSRGIIAALGPDGKPS g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g

5220 255 260 265 270 RIVVIYTTGSQATMDERNRQ AmpR RIVVIYTTGSQATMDERNRQ c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c

5280 275 280 285 IAEIGASLIKHW* AmpR IAEIGASLIKHW* a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t

5340 t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g

5400 c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t

5460 ori g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g

5520 ori t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t

5580 ori c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a

5640 ori c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g

5700 ori g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c

5760 ori t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c

5820 ori t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c

5880 ori c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g

5940 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a

6000 ori a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c

6060 ori g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g

AflIIIPciINspI 6120 t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a

6180 a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t

BspQISapI 6200 g t g a g c g a g g a a g c g g a a g a c a c t c g c t c c t t c g c c t t c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.