Addgene pAK011 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAK011 Sequencing Result


Map View

PfoI (46) BtgZI (2578) BsrDI (2559) pENTR-R (2473 .. 2492) NruI (2398) Kan-R (2395 .. 2414) EcoNI (2142) SspI (2130) BsrFI (2097) AsiSI (2057) Bpu10I - BsmBI - Esp3I (2035) TaqII (1803) PflMI (1795) AcuI - Eco57MI (1393) AlwNI (1261) PspFI (1153) pGEX 3' (29 .. 51) ZraI (53) AatII (55) XcmI (113) BtgI (136) CAP binding site M13/pUC Reverse (316 .. 338) BsrBI (317) lac operator M13 Reverse (335 .. 351) HindIII (364) BfuAI - BspMI (369) SphI (374) PstI - SbfI (380) SalI (382) AccI (383) HincII (384) XbaI (388) BamHI (394) Acc65I (403) KpnI (407) Eco53kI (411) SacI (413) EcoRI (415) EaeI (423) M13 Forward (421 .. 438) M13/pUC Forward (430 .. 452) BmrI (457) FspI (557) BglI (567) KasI (576) NarI (577) SfoI (578) PluTI (580) NdeI (629) BstAPI (633) TatI (644) pRS-marker (646 .. 665) AflIII - PciI (845) pBR322ori-F (986 .. 1005) BssSI - BssSαI (1018) BciVI (1048) BseYI (1149) pAK011 2594 bp

Sequence View

ZraIAatIIPfoI 60 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a c g t c a t t g a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t g c a g t a a c

XcmI 120 t c g a t g g t c t c a g a a a a a g t g g c a c c g a g t c g g t g c a a c a a a g c a c c a g t g g t c t a g t g g a g c t a c c a g a g t c t t t t t c a c c g t g g c t c a g c c a c g t t g t t t c g t g g t c a c c a g a t c a c c

BtgI 180 t a g a a t a g t a c c c t g c c a c g g t a c a g a c c c g g g t t c g a t t c c c g g c t g g t g c a t t g t c t t a t c t t a t c a t g g g a c g g t g c c a t g t c t g g g c c c a a g c t a a g g g c c g a c c a c g t a a c a g a a

240 CAP binding site c g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a

300 -35 -10 CAP binding site lac promoter g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c

BsrBI 360 1 5 MTMIT lac promoter lac operator lacZ α M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g

HindIIIBspMIBfuAISphISbfIPstISalIAccIHincIIXbaIBamHIAcc65IKpnIEco53kISacIEcoRI 420 10 15 20 25 PSLHACRSTLEDPRVPSSNS lacZ α MCS g c c a a g c t t g c a t g c c t g c a g g t c g a c t c t a g a g g a t c c c c g g g t a c c g a g c t c g a a t t c c g g t t c g a a c g t a c g g a c g t c c a g c t g a g a t c t c c t a g g g g c c c a t g g c t c g a g c t t a a g

BmrIEaeI 480 30 35 40 45 LAVVLQRRDWENPGVTQLNR lacZ α M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c

540 50 55 60 65 LAAHPPFASWRNSEEARTDR lacZ α c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c

KasINarISfoIPluTIBglIFspI 600 70 75 80 85 PSQQLRSLNGEWRLMRYFLL lacZ α c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g c g c c t g a t g c g g t a t t t t c t c c t g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c g c g g a c t a c g c c a t a a a a g a g g a

TatIBstAPINdeI 660 90 95 100 105 THLCGISHRIWCTLSTICSD lacZ α A T G T T A G A C G A G A C T pRS-marker t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t

720 AA* lacZ α A C G G C pRS-marker t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g

780 t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c g a a g a c a a g t t t t a g a g c t a g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g c t t c t g t t c a a a a t c t c g a t c

840 a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t g t g a g a c c a t c a t t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a c a c t c t g g t a g t a

AflIIIPciI 900 t g t c a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g a c a g t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c

960 ori g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c

BssSαIBssSI 1020 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g

BciVI 1080 ori g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c

1140 ori g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a

BseYIPspFI 1200 ori c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g

1260 ori g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g

AlwNI 1320 ori a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c

1380 ori t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t

Eco57MIAcuI 1440 ori g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g

1500 ori g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a

1560 c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a

1620 t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a

1680 270 *FF KanR *FF t t t a a a t c a a g c c c a a t c t g a a t a a t g t t a c a a c c a a t t a a c c a a t t c t g a t t a g a a a a a a a a t t t a g t t c g g g t t a g a c t t a t t a c a a t g t t g g t t a a t t g g t t a a g a c t a a t c t t t t t

1740 265 260 255 250 EDLMLHFQLKNMDPNDIGYK KanR EDLMLHFQLKNMDPNDIGYK c t c a t c g a g c a t c a a a t g a a a c t g c a a t t t a t t c a t a t c a g g a t t a t c a a t a c c a t a t t t g a g t a g c t c g t a g t t t a c t t t g a c g t t a a a t a a g t a t a g t c c t a a t a g t t a t g g t a t a a a

PflMI 1800 245 240 235 230 QFLRKQLSPSFEGLCNWLIA KanR QFLRKQLSPSFEGLCNWLIA t t g a a a a a g c c g t t t c t g t a a t g a a g g a g a a a a c t c a c c g a g g c a g t t c c a t a g g a t g g c a a c t t t t t c g g c a a a g a c a t t a c t t c c t c t t t t g a g t g g c t c c g t c a a g g t a t c c t a c c g

TaqII 1860 225 220 215 210 LDQYRDAIGVRGVDICGILK KanR LDQYRDAIGVRGVDICGILK a a g a t c c t g g t a t c g g t c t g c g a t t c c g a c t c g t c c a a c a t c a a t a c a a c c t a t t a a t t t t t c t a g g a c c a t a g c c a g a c g c t a a g g c t g a g c a g g t t g t a g t t a t g t t g g a t a a t t a a a

1920 205 200 195 190 GEDFILNDLSFDGHTVVSDP KanR GEDFILNDLSFDGHTVVSDP c c c c t c g t c a a a a a t a a g g t t a t c a a g t g a g a a a t c a c c a t g a g t g a c g a c t g a a t c c g g g g g g a g c a g t t t t t a t t c c a a t a g t t c a c t c t t t a g t g g t a c t c a c t g c t g a c t t a g g c c

1980 185 180 175 170 SFPLLKHMEKWVQEVPWGNR KanR SFPLLKHMEKWVQEVPWGNR t g a g a a t g g c a a a a g t t t a t g c a t t t c t t t c c a g a c t t g t t c a a c a g g c c a g c c a t t a c g a c t c t t a c c g t t t t c a a a t a c g t a a a g a a a g g t c t g a a c a a g t t g t c c g g t c g g t a a t g c

Bpu10IBsmBIEsp3I 2040 165 160 155 150 EDDFDSADVLGNNMRSQAQA KanR EDDFDSADVLGNNMRSQAQA c t c g t c a t c a a a a t c a c t c g c a t c a a c c a a a c c g t t a t t c a t t c g t g a t t g c g c c t g a g c g a g c a g t a g t t t t a g t g a g c g t a g t t g g t t t g g c a a t a a g t a a g c a c t a a c g c g g a c t c g

BsrFIAsiSI 2100 145 140 135 130 LRFVRDSNFPCNCVPISHLR KanR LRFVRDSNFPCNCVPISHLR g a g a c g a a a t a c g c g a t c g c t g t t a a a a g g a c a a t t a c a a a c a g g a a t c g a a t g c a a c c g c t c t g c t t t a t g c g c t a g c g a c a a t t t t c c t g t t a a t g t t t g t c c t t a g c t t a c g t t g g c

EcoNISspI 2160 125 120 115 110 RLFVALADVINEGSDPYEEL KanR RLFVALADVINEGSDPYEEL g c g c a g g a a c a c t g c c a g c g c a t c a a c a a t a t t t t c a c c t g a a t c a g g a t a t t c t t c t a a c g c g t c c t t g t g a c g g t c g c g t a g t t g t t a t a a a a g t g g a c t t a g t c c t a t a a g a a g a t t

2220 105 100 95 90 VQFATKGPIATTLLWADDPT KanR VQFATKGPIATTLLWADDPT t a c c t g g a a t g c t g t t t t t c c g g g g a t c g c a g t g g t g a g t a a c c a t g c a t c a t c a g g a g t a t g g a c c t t a c g a c a a a a a g g c c c c t a g c g t c a c c a c t c a t t g g t a c g t a g t a g t c c t c a

2280 85 80 75 70 RIFHKITPLPMFETLWNLRV KanR RIFHKITPLPMFETLWNLRV a c g g a t a a a a t g c t t g a t g g t c g g a a g a g g c a t a a a t t c c g t c a g c c a g t t t a g t c t g a c t g c c t a t t t t a c g a a c t a c c a g c c t t c t c c g t a t t t a a g g c a g t c g g t c a a a t c a g a c t g

2340 65 60 55 50 MEDTVDNAVSGKGHKLFLEP KanR MEDTVDNAVSGKGHKLFLEP c a t c t c a t c t g t a a c a t c a t t g g c a a c g c t a c c t t t g c c a t g t t t c a g a a a c a a c t c t g g g t a g a g t a g a c a t t g t a g t a a c c g t t g c g a t g g a a a c g g t a c a a a g t c t t t g t t g a g a c c

NruI 2400 45 40 35 30 ADPKGYLRYITAGSQGVNDR KanR A T C G C G Kan-R ADPKGYLRYITAGSQGVNDR c g c a t c g g g c t t c c c a t a c a a g c g a t a g a t t g t c g c a c c t g a t t g c c c g a c a t t a t c g c g g c g t a g c c c g a a g g g t a t g t t c g c t a t c t a a c a g c g t g g a c t a a c g g g c t g t a a t a g c g c

2460 25 20 15 10 AWKYGYLDADMNSNLRPRST KanR A G C C C A T T T A T A C C Kan-R AWKYGYLDADMNSNLRPRST a g c c c a t t t a t a c c c a t a t a a a t c a g c a t c c a t g t t g g a a t t t a a t c g c g g c c t c g a c g t t c g g g t a a a t a t g g g t a t a t t t a g t c g t a g g t a c a a c c t t a a a t t a g c g c c g g a g c t g c a

2520 5 1 ERQIHSM KanR A T G G C T C A T A A C A C C C C T T G pENTR-R ERQIHSM t t c c c g t t g a a t a t g g c t c a t a a c a c c c c t t g t a t t a c t g t t t a t g t a a g c a g a c a g t t t a a g g g c a a c t t a t a c c g a g t a t t g t g g g g a a c a t a a t g a c a a a t a c a t t c g t c t g t c a a a

BtgZIBsrDI 2580 t a t t g t t c a t g a t g a t a t a t t t t t a t c t t g t g c a a t g t a a c a t c a g a g a t t t t g a g a c a c a t a a c a a g t a c t a c t a t a t a a a a a t a g a a c a c g t t a c a t t g t a g t c t c t a a a a c t c t g t g

2594 g g g c c a g a g c t g c a c c c g g t c t c g a c g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.