Addgene pLVTHM-shRnd3 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pLVTHM-shRnd3 Sequencing Result


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EBV-rev (10,946 .. 10,965) SV40pA-R (10,892 .. 10,911) SV40 NLS small t intron PshAI (9896) AvrII (9066) SfiI (9019) pBABE 3' (8747 .. 8767) L4440 (8660 .. 8677) pBR322ori-F (8407 .. 8426) AhdI (7674) FspI (7451) PvuI (7305) Amp-R (7105 .. 7124) pBRforEco (6696 .. 6714) pGEX 3' (6636 .. 6658) pRS-marker (6517 .. 6536) pBRrevBam (6284 .. 6303) SgrAI (6256) SP6 (5977 .. 5994) SP6 promoter XbaI (5955) 5' LTR (truncated) loxP MluI (5682) PmlI (291) 3' LTR HIV-1 Ψ EagI - NotI (1145) KflI - PpuMI (1931) BfuAI - BspMI (2671) EF-1α promoter FseI (2851) PasI (2895) EF1a-F (3273 .. 3293) SwaI (3340) AbsI (3509) PmeI (3518) MauBI (3551) EGFP-N (3626 .. 3647) EXFP-R (3887 .. 3906) EGFP-C (4234 .. 4255) SpeI (4358) NdeI (4365) WPRE-R (4425 .. 4445) SnaBI (4968) MscI * (4974) Acc65I (4978) KpnI (4982) BstXI (5103) BamHI (5106) tet operator tet operator tet operator tet operator tet operator tet operator EcoRI (5379) H1 (5520 .. 5539) pLVTHM-shRnd3 11,155 bp

Sequence View

5ʹ 3ʹ 60 3' LTR t t g g a a g g g c t a a t t c a c t c c c a a a g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a a c c t t c c c g a t t a a g t g a g g g t t t c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g

120 3' LTR a c a c a a g g c t a c t t c c c t g a t t a g c a g a a c t a c a c a c c a g g g c c a g g g g t c a g a t a t c c a t g t g t t c c g a t g a a g g g a c t a a t c g t c t t g a t g t g t g g t c c c g g t c c c c a g t c t a t a g g t

180 3' LTR c t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c c a g a t a a g g t a g a a g a g g c c g a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g g t c t a t t c c a t c t t c t c c g g

240 3' LTR a a t a a a g g a g a g a a c a c c a g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g t t a t t t c c t c t c t t g t g g t c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c

PmlI 300 3' LTR g a g a g a g a a g t g t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c g t g g c c c g a c t c t c t c t t c a c a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g c a c c g g g c t

360 3' LTR g a g c t g c a t c c g g a g t a c t t c a a g a a c t g c t g a t a t c g a g c t t g c t a c a a g g g a c t t t c c c t c g a c g t a g g c c t c a t g a a g t t c t t g a c g a c t a t a g c t c g a a c g a t g t t c c c t g a a a g g

420 3' LTR g c t g g g g a c t t t c c a g g g a g g c g t g g c c t g g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a c g a c c c c t g a a a g g t c c c t c c g c a c c g g a c c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t

480 3' LTR t c c t g c a t a t a a g c a g c t g c t t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g g a c g t a t a t t c g t c g a c g a a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c

540 3' LTR a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g

600 3' LTR t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a

660 3' LTR c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t

720 HIV-1 Ψ g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c

780 HIV-1 Ψ g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c

840 HIV-1 Ψ a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c

900 g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c

960 g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g

1020 t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t

1080 t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g

1140 a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c

EagINotI 1200 MRDNWRSE c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t

1260 LYKYKVVKIEPLGVAPTKAK a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t

1320 RRE RRVVQREKRAVGIGALFLGF g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a

1380 RRE LGAAGSTMGAASMTLTVQAR c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c

1440 RRE QLLSGIVQQQNNLLRAIEAQ a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t

1500 RRE QHLLQLTVWGIKQLQARILA a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g

1560 RRE VERYLKDQQLLGIWGCSGKL t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a

1620 ICTTAVPWNASWSNKSLEQI c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a

1680 WNHTTWMEWDREINNYTSLI t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a

1740 1 5 KNEQELLE gp41 peptide HSLIEESQNQQEKNEQELLE a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t

1800 10 15 LDKWASL (in frame with gp41 peptide) WNWFNITNWLWYI gp41 peptide LDKWASLWNWFNITNWLWYI a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a

1860 (in frame with gp41 peptide) KLFIMIVGGLVGLRIVFAVL KLFIMIVGGLVGLRIVFAVL a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a

1920 (in frame with gp41 peptide) SIVNRVRQGYSPLSFQTHLP SIVNRVRQGYSPLSFQTHLP t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g

PpuMIKflI 1980 (in frame with gp41 peptide) TPRGPDRPEGIEEEGGERDR TPRGPDRPEGIEEEGGERDR a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c

2040 (in frame with gp41 peptide) DRSIRLVNGSRRYRSRD* DRSIRLVNGSRRYRSRD* a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g a t c a c g a g a c t a g c c t c g t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c t a g t g c t c t g a t c g g a g c

2100 a c c a t c g c g a t g t c g a c g a t a a g c t t t g c a a a g a t g g a t a a a g t t t t a a a c a g a g a g g a a t g g t a g c g c t a c a g c t g c t a t t c g a a a c g t t t c t a c c t a t t t c a a a a t t t g t c t c t c c t t

2160 EF-1 α promoter t c t t t g c a g c t a a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t g g c t c c g g t g c c c g a g a a a c g t c g a t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a c c g a g g c c a c g g g c

2220 EF-1 α promoter t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t

2280 EF-1 α promoter t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c

2340 EF-1 α promoter g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t

2400 EF-1 α promoter EF-1 α intron A c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c

2460 EF-1 α promoter EF-1 α intron A *KWRA c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c t g g c t g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g a c c g a

2520 EF-1 α promoter EF-1 α intron A ATRSEQDRAEPQFHTPSNSA g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a g g c c c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c t c c g g

2580 EF-1 α promoter EF-1 α intron A KRKLLGKAEHKLQPRAQASP t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t g g g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g a c c c c

2640 EF-1 α promoter EF-1 α intron A GGRAFRTAGERRDRQKRYTE c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a

BspMIBfuAI 2700 EF-1 α promoter EF-1 α intron A LWKFNKIVQQSAKKRALYDQ a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a

2760 EF-1 α promoter EF-1 α intron A LHPGLDACQYKPKQPRPRRR a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c

2820 EF-1 α promoter EF-1 α intron A PGHTGACMNPSAPGALAAVS g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c

FseI 2880 EF-1 α promoter EF-1 α intron A FRVPTTELQGAQEPAQGRAA a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g

PasI 2940 EF-1 α promoter EF-1 α intron A TYRGARPPLAPGTPVLQTLP g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t

3000 EF-1 α promoter EF-1 α intron A FIAAERGQQLSSLISSAASP a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c t c g g g t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g a g c c c

3060 EF-1 α promoter EF-1 α intron A LAPPHTVWVFSFPRETRLRR a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c g c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g c a g c g

3120 EF-1 α promoter EF-1 α intron A KM t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g a g c t t a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c t c g a a

3180 EF-1 α promoter EF-1 α intron A t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g

3240 EF-1 α promoter EF-1 α intron A t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a

3300 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a

SwaI 3360 EF-1 α promoter EF-1 α intron A t t t t c t t c c a t t t c a g g t g t c g t g a g g a a t t t c g a c a t t t a a a t t t a a t t a a t c t c g a c g a a a a g a a g g t a a a g t c c a c a g c a c t c c t t a a a g c t g t a a a t t t a a a t t a a t t a g a g c t g c

3420 cPPT/CTS g t a t c g g t t a a c t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t c a t a g c c a a t t g a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a

3480 cPPT/CTS a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a

PmeIAbsI 3540 cPPT/CTS t c a a a a t t t t a t c g a t c a c g a g a c t a g c c t c g a g g t t t a a a c t a c g g g a t c t t c g a a g g c a g t t t t a a a a t a g c t a g t g c t c t g a t c g g a g c t c c a a a t t t g a t g c c c t a g a a g c t t c c g

MauBI 3600 1 M 1a V 5 SKGEE EGFP 1 M 1a V 5 SKGEE EGFP Kozak sequence MVSKGEE c t a a g c t t a c g c g c g c g t c c t a g c g c t a c c g g t c g c c a c c a t g g t g a g c a a g g g c g a g g a g a t t c g a a t g c g c g c g c a g g a t c g c g a t g g c c a g c g g t g g t a c c a c t c g t t c c c g c t c c t

3660 10 15 20 25 LFTGVVPILVELDGDVNGHK EGFP 10 15 20 25 LFTGVVPILVELDGDVNGHK EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N LFTGVVPILVELDGDVNGHK g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t

3720 30 35 40 45 FSVSGEGEGDATYGKLTLKF EGFP 30 35 40 45 FSVSGEGEGDATYGKLTLKF EGFP FSVSGEGEGDATYGKLTLKF g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a

3780 50 55 60 65 ICTTGKLPVPWPTLVTTLTY EGFP 50 55 60 65 ICTTGKLPVPWPTLVTTLTY EGFP ICTTGKLPVPWPTLVTTLTY c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t

3840 70 75 80 85 GVQCFSRYPDHMKQHDFFKS EGFP 70 75 80 85 GVQCFSRYPDHMKQHDFFKS EGFP GVQCFSRYPDHMKQHDFFKS c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g

3900 90 95 100 105 AMPEGYVQERTIFFKDDGNY EGFP 90 95 100 105 AMPEGYVQERTIFFKDDGNY EGFP C T G C T G C C G T T G A T EXFP-R AMPEGYVQERTIFFKDDGNY c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t

3960 110 115 120 125 KTRAEVKFEGDTLVNRIELK EGFP 110 115 120 125 KTRAEVKFEGDTLVNRIELK EGFP G T T C T G EXFP-R KTRAEVKFEGDTLVNRIELK c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t

4020 130 135 140 145 GIDFKEDGNILGHKLEYNYN EGFP 130 135 140 145 GIDFKEDGNILGHKLEYNYN EGFP GIDFKEDGNILGHKLEYNYN g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t

4080 150 155 160 165 SHNVYIMADKQKNGIKVNFK EGFP 150 155 160 165 SHNVYIMADKQKNGIKVNFK EGFP SHNVYIMADKQKNGIKVNFK c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t

4140 170 175 180 185 IRHNIEDGSVQLADHYQQNT EGFP 170 175 180 185 IRHNIEDGSVQLADHYQQNT EGFP IRHNIEDGSVQLADHYQQNT g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g

4200 190 195 200 205 PIGDGPVLLPDNHYLSTQSA EGFP 190 195 200 205 PIGDGPVLLPDNHYLSTQSA EGFP PIGDGPVLLPDNHYLSTQSA c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g

4260 210 215 220 225 LSKDPNEKRDHMVLLEFVTA EGFP 210 215 220 225 LSKDPNEKRDHMVLLEFVTA EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C LSKDPNEKRDHMVLLEFVTA c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g

4320 230 235 AGITLGMDELYK (in frame with EGFP) SGLRSRLA EGFP 230 235 AGITLGMDELYK (in frame with EGFP) SGLRSRLA EGFP AGITLGMDELYKSGLRSRLA c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t c c g g a c t c a g a t c t c g a c t a g c g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a g g c c t g a g t c t a g a g c t g a t c g

NdeISpeI 4380 SS* WPRE SS* SS* t a g t a g c t a g c t a g c t a g t c g a g c t c a a g c t t c g g g g a c t a g t c a t a t g a t a a t c a a c c t a t c a t c g a t c g a t c g a t c a g c t c g a g t t c g a a g c c c c t g a t c a g t a t a c t a t t a g t t g g a

4440 WPRE A C A A C G A G G A A A A T G C WPRE-R c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c

4500 WPRE (in frame with Factor Xa site) *HRQIMSNSGTHSE G A T A C WPRE-R MPLYHAIASRMAF *HRQIMSNSGTHSE c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g

4560 WPRE (in frame with Factor Xa site) NEGGQIFGPQQRKILLQPGN IFSSLYKSWLLSLYEELWPV NEGGQIFGPQQRKILLQPGN a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a

4620 WPRE (in frame with Factor Xa site) DPLTAHHASHKSVCGGSTPA VRQRGVVCTVFADATPTGWG DPLTAHHASHKSVCGGSTPA g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g

4680 WPRE (in frame with Factor Xa site) NGGGTLEKGPSESEGERNGR IATTCQLLSGTFAFPLPIAT NGGGTLEKGPSESEGERNGR a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c

4740 WPRE (in frame with Factor Xa site) RFEDGGAKGAAPCPSPQQAS AELIAACLARCWTGARLLGT RFEDGGAKGAAPCPSPQQAS g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a

4800 WPRE (in frame with Factor Xa site) VIGHHQRPLQRGKWPQEGTN DNSVVLSGKLTSFPWLLACV VIGHHQRPLQRGKWPQEGTN g a c a a t t c c g t g g t g t t g t c g g g g a a g c t g a c g t c c t t t c c a t g g c t g c t c g c c t g t g t t c t g t t a a g g c a c c a c a a c a g c c c c t t c g a c t g c a g g a a a g g t a c c g a c g a g c g g a c a c a a

4860 WPRE (in frame with Factor Xa site) GGPNQAPRGEAVDR 1 RGEI Factor Xa site ATWILRGTSFCYVPSALNPA GGPNQAPRGEAVDRRGEIWR g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c

4920 WPRE DLPSRGLLPALRPLPRLRLR VKRGAAQQRSQPRKRTKAKA g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g

Acc65IMscI*SnaBI 4980 WPRE PQTSRISLWAASPHRYVWPG RLRTPDGKPGGGRM c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g g t a c g t a t g g c c a g g t g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c c a t g c a t a c c g g t c c a

KpnI 5040 TFKTNDLQGSCRS* a c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a t g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t

5100 g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t g g g a t c a a t t c a c c a t g c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a c c c t a g t t a a g t g g t a c

BstXIBamHI 5160 tet operator c t a g t g g a t c c c c c t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g a t c a c c t a g g g g g a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t

5220 tet operator tet operator g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t c c c t a t c a c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a g g g a t a g t

5280 tet operator tet operator g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t

5340 tet operator tet operator a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t c c t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a g g

EcoRI 5400 tet operator H1 promoter c t a t c a g t g a t a g a g a a a a g t g a a a g t c g g g g c t g c a g g a a t t c g a a c g c t g a c g t c a t c g a t a g t c a c t a t c t c t t t t c a c t t t c a g c c c c g a c g t c c t t a a g c t t g c g a c t g c a g t a g

5460 H1 promoter a a c c c g c t c c a a g g a a t c g c g g g c c c a g t g t c a c t a g g c g g g a a c a c c c a g c g c g c g t g c t t g g g c g a g g t t c c t t a g c g c c c g g g t c a c a g t g a t c c g c c c t t g t g g g t c g c g c g c a c g

5520 H1 promoter T H1 g c c c t g g c a g g a a g a t g g c t g t g a g g g a c a g g g g a g t g g c g c c c t g c a a t a t t t g c a t g t c g g g a c c g t c c t t c t a c c g a c a c t c c c t g t c c c c t c a c c g c g g g a c g t t a t a a a c g t a c a

5580 H1 promoter C G C T A T G T G T T C T G G G A A A H1 c g c t a t g t g t t c t g g g a a a t c a c c a t a a a c g t g a a a t g t c t t t g g a t t t g g g a a t c t t a t g c g a t a c a c a a g a c c c t t t a g t g g t a t t t g c a c t t t a c a g a a a c c t a a a c c c t t a g a a t a

5640 H1 promoter a a g t t c t g t a t g a g a c c a c a a g c t t c c t a g t a g a g c t c t c c a a t c a c a t t c a a g a g a t g t t t c a a g a c a t a c t c t g g t g t t c g a a g g a t c a t c t c g a g a g g t t a g t g t a a g t t c t c t a c a

MluI 5700 g a t t g g a g a g c t c t a c t a t t t t t g g a a g t c g a c t t a a t t a a a c g c g t c t c c a a g c t t a t c c t a a c c t c t c g a g a t g a t a a a a a c c t t c a g c t g a a t t a a t t t g c g c a g a g g t t c g a a t a g

5760 loxP g a t a c c g t c g c a t g g g a a t a a c t t c g t a t a g c a t a c a t t a t a c g a a g t t a t g c t g c t t t t c t a t g g c a g c g t a c c c t t a t t g a a g c a t a t c g t a t g t a a t a t g c t t c a a t a c g a c g a a a a

5820 5' LTR (truncated) t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a

5880 5' LTR (truncated) a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g

5940 5' LTR (truncated) c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t

XbaI 6000 5' LTR (truncated) SP6 promoter G A T A T C A C A G T G G A T T T A SP6 a a t c t c t a g c a g c a t c t a g a a t t a a t t c c g t g t a t t c t a t a g t g t c a c c t a a a t c g t a t g t t a g a g a t c g t c g t a g a t c t t a a t t a a g g c a c a t a a g a t a t c a c a g t g g a t t t a g c a t a c

6060 t g t a t g a t a c a t a a g g t t a t g t a t t a a t t g t a g c c g c g t t c t a a c g a c a a t a t g t a c a a g a c a t a c t a t g t a t t c c a a t a c a t a a t t a a c a t c g g c g c a a g a t t g c t g t t a t a c a t g t t c

6120 c c t a a t t g t g t a g c a t c t g g c t t a c t g a a g c a g a c c c t a t c a t c t c t c t c g t a a a c t g c c g g a t t a a c a c a t c g t a g a c c g a a t g a c t t c g t c t g g g a t a g t a g a g a g a g c a t t t g a c g g

6180 g t c a g a g t c g g t t t g g t t g g a c g a a c c t t c t g a g t t t c t g g t a a c g c c g t c c c g c a c c c g c a g t c t c a g c c a a a c c a a c c t g c t t g g a a g a c t c a a a g a c c a t t g c g g c a g g g c g t g g g c

6240 g a a a t g g t c a g c g a a c c a a t c a g c a g g g t c a t c g c t a g c c a g a t c c t c t a c g c c g g a c g c c t t t a c c a g t c g c t t g g t t a g t c g t c c c a g t a g c g a t c g g t c t a g g a g a t g c g g c c t g c g

SgrAI 6300 G G A T A T A G C G G C T G T A G pBRrevBam a t c g t g g c c g g c a t c a c c g g c g c c a c a g g t g c g g t t g c t g g c g c c t a t a t c g c c g a c a t c t a g c a c c g g c c g t a g t g g c c g c g g t g t c c a c g c c a a c g a c c g c g g a t a t a g c g g c t g t a g

6360 T G G pBRrevBam a c c g a t g g g g a a g a t c g g g c t c g c c a c t t c g g g c t c a t g a g c g c t t g t t t c g g c g t g g g t t g g c t a c c c c t t c t a g c c c g a g c g g t g a a g c c c g a g t a c t c g c g a a c a a a g c c g c a c c c a

6420 a t g g t g g c a g g c c c c g t g g c c g g g g g a c t g t t g g g c g c c a t c t c c t t g c a t g c a c c a t t c t a c c a c c g t c c g g g g c a c c g g c c c c c t g a c a a c c c g c g g t a g a g g a a c g t a c g t g g t a a g

6480 c t t g c g g c g g c g g t g c t c a a c g g c c t c a a c c t a c t a c t g g g c t g c t t c c t a a t g c a g g a g g a a c g c c g c c g c c a c g a g t t g c c g g a g t t g g a t g a t g a c c c g a c g a a g g a t t a c g t c c t c

6540 A T G T T A G A C G A G A C T A C G G C pRS-marker t c g c a t a a g g g a g a g c g t c g a a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a a g c g t a t t c c c t c t c g c a g c t t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t

6600 g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a

6660 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a

6720 C G G A G C A C T A T G C G G A T A A pBRforEco t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t

6780 g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a

6840 AmpR promoter g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c

6900 1 5 signal sequence MSIQH AmpR promoter AmpR MSIQH a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t

6960 10 15 20 signal sequence FRVALIPFFAAFCLPVFA 25 HP AmpR FRVALIPFFAAFCLPVFAHP t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g

7020 30 35 40 45 ETLVKVKDAEDQLGARVGYI AmpR ETLVKVKDAEDQLGARVGYI a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a

7080 50 55 60 65 ELDLNSGKILESFRPEERFP AmpR ELDLNSGKILESFRPEERFP c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g

7140 70 75 80 85 MMSTFKVLLCGAVLSRIDAG AmpR C G A T A C A C C G C G C C A T A A T A Amp-R MMSTFKVLLCGAVLSRIDAG a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c

7200 90 95 100 105 QEQLGRRIHYSQNDLVEYSP AmpR QEQLGRRIHYSQNDLVEYSP g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g

7260 110 115 120 125 VTEKHLTDGMTVRELCSAAI AmpR VTEKHLTDGMTVRELCSAAI a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a

PvuI 7320 130 135 140 145 TMSDNTAANLLLTTIGGPKE AmpR TMSDNTAANLLLTTIGGPKE a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t

7380 150 155 160 165 LTAFLHNMGDHVTRLDRWEP AmpR LTAFLHNMGDHVTRLDRWEP g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g

7440 170 175 180 185 ELNEAIPNDERDTTMPVAMA AmpR ELNEAIPNDERDTTMPVAMA g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g

FspI 7500 190 195 200 205 TTLRKLLTGELLTLASRQQL AmpR TTLRKLLTGELLTLASRQQL a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a

7560 210 215 220 225 IDWMEADKVAGPLLRSALPA AmpR IDWMEADKVAGPLLRSALPA a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g

7620 230 235 240 245 GWFIADKSGAGERGSRGIIA AmpR GWFIADKSGAGERGSRGIIA t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g

AhdI 7680 250 255 260 265 ALGPDGKPSRIVVIYTTGSQ AmpR ALGPDGKPSRIVVIYTTGSQ a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t

7740 270 275 280 285 ATMDERNRQIAEIGASLIKH AmpR ATMDERNRQIAEIGASLIKH g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t

7800 W* AmpR W* t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a

7860 t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t

7920 ori a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c

7980 ori a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g

8040 ori g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c

8100 ori c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t

8160 ori g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g

8220 ori c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g

8280 ori g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t

8340 ori c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c

8400 ori a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a

8460 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t

8520 ori g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g

8580 g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a

8640 a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c

8700 A G C G A G T C A G T G A G C G A G L4440 c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c

8760 SV40 promoter C C T T A C A C A C A G T C pBABE 3' c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g t g g a a t g t g t g t c a g g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c a c c t t a c a c a c a g t c

8820 SV40 promoter A A T C C C A pBABE 3' t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g

8880 SV40 promoter a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t

8940 SV40 promoter SV40 ori a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g

9000 SV40 promoter SV40 ori c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a

SfiI 9060 SV40 promoter SV40 ori g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a

AvrII 9120 SV40 promoter SV40 ori g g a g g c c t a g g c t t t t g c a a a a a g c t t g g a c a c a a g a c a g g c t t g c g a g a t a t g t t t g a g c c t c c g g a t c c g a a a a c g t t t t t c g a a c c t g t g t t c t g t c c g a a c g c t c t a t a c a a a c t c

9180 a a t a c c a c t t t a t c c c g c g t c a g g g a g a g g c a g t g c g t a a a a a g a c g c g g a c t c a t g t g a t t a t g g t g a a a t a g g g c g c a g t c c c t c t c c g t c a c g c a t t t t t c t g c g c c t g a g t a c a c t

9240 a a t a c t g g t t t t t a g t g c g c c a g a t c t c t a t a a t c t c g c g c a a c c t a t t t t c c c c t c g a a t t a t g a c c a a a a a t c a c g c g g t c t a g a g a t a t t a g a g c g c g t t g g a t a a a a g g g g a g c t t

9300 c a c t t t t t a a g c c g t a g a t a a a c a g g c t g g g a c a c t t c a c a t g a g c g a a a a a t a c a t c g t g t g a a a a a t t c g g c a t c t a t t t g t c c g a c c c t g t g a a g t g t a c t c g c t t t t t a t g t a g c a

9360 c a c c t g g g a c a t g t t g c a g a t c c a t g c a c g t a a a c t c g c a a g c c g a c t g a t g c c t t c t g a g t g g a c c c t g t a c a a c g t c t a g g t a c g t g c a t t t g a g c g t t c g g c t g a c t a c g g a a g a c t

9420 a c a a t g g a a a g g c a t t a t t g c c g t a a g c c g t g g c g g t c t g t a c c g g g t g c g t t a c t g g c g t g t t a c c t t t c c g t a a t a a c g g c a t t c g g c a c c g c c a g a c a t g g c c c a c g c a a t g a c c g c

9480 c g t g a a c t g g g t a t t c g t c a t g t c g a t a c c g t t t g t a t t t c c a g c t a c g a t c a c g a c a a c g c a c t t g a c c c a t a a g c a g t a c a g c t a t g g c a a a c a t a a a g g t c g a t g c t a g t g c t g t t g

9540 c a g c g c g a g c t t a a a g t g c t g a a a c g c g c a g a a g g c g a t g g c g a a g g c t t c a t c g t t a t t g t c g c g c t c g a a t t t c a c g a c t t t g c g c g t c t t c c g c t a c c g c t t c c g a a g t a g c a a t a a

9600 g a t g a c c t g g t g g a t a c c g g t g g t a c t g c g g t t g c g a t t c g t g a a a t g t a t c c a a a a g c g c t a c t g g a c c a c c t a t g g c c a c c a t g a c g c c a a c g c t a a g c a c t t t a c a t a g g t t t t c g c

9660 c a c t t t g t c a c c a t c t t c g c a a a a c c g g c t g g t c g t c c g c t g g t t g a t g a c t a t g t t g t t g t g a a a c a g t g g t a g a a g c g t t t t g g c c g a c c a g c a g g c g a c c a a c t a c t g a t a c a a c a a

9720 g a t a t c c c g c a a g a t a c c t g g a t t g a a c a g c c g t g g g a t a t g g g c g t c g t a t t c g t c c c g c t a t a g g g c g t t c t a t g g a c c t a a c t t g t c g g c a c c c t a t a c c c g c a g c a t a a g c a g g g c

9780 c c a a t c t c c g g t c g c t a a t c t t t t c a a c g c c t g g c a c t g c c g g g c g t t g t t c t t t t t a a c g g t t a g a g g c c a g c g a t t a g a a a a g t t g c g g a c c g t g a c g g c c c g c a a c a a g a a a a a t t g

9840 t t c a g g c g g g t t a c a a t a g t t t c c a g t a a g t a t t c t g g a g g c t g c a t c c a t g a c a c a g g c a a g t c c g c c c a a t g t t a t c a a a g g t c a t t c a t a a g a c c t c c g a c g t a g g t a c t g t g t c c g

PshAI 9900 a a a c c t g a g c g a a a c c c t g t t c a a a c c c c g c t t t a a a c a t c c t g a a a c c t c g a c g c t a g t t t t g g a c t c g c t t t g g g a c a a g t t t g g g g c g a a a t t t g t a g g a c t t t g g a g c t g c g a t c a

9960 c c g c c g c t t t a a t c a c g g c g c a c a a c c g c c t g t g c a g t c g g c c c t t g a t g g t a a a a c c a t g g c g g c g a a a t t a g t g c c g c g t g t t g g c g g a c a c g t c a g c c g g g a a c t a c c a t t t t g g t a

10,020 c c c t c a c t g g t a t c g c a t g a t t a a c c g t c t g a t g t g g a t c t g g c g c g g c a t t g a c c c a c g g g g a g t g a c c a t a g c g t a c t a a t t g g c a g a c t a c a c c t a g a c c g c g c c g t a a c t g g g t g c

10,080 c g a a a t c c t c g a c g t c c a g g c a c g t a t t g t g a t g a g c g a t g c c g a a c g t a c c g a c g a t g a g c t t t a g g a g c t g c a g g t c c g t g c a t a a c a c t a c t c g c t a c g g c t t g c a t g g c t g c t a c t

10,140 t t t a t a c g a t a c g g t g a t t g g c t a c c g t g g c g g c a a c t g g a t t t a t g a g t g g g c c c c g g a a a a t a t g c t a t g c c a c t a a c c g a t g g c a c c g c c g t t g a c c t a a a t a c t c a c c c g g g g c c t

10,200 t c t t t g t g a a g g a a c c t t a c t t c t g t g g t g t g a c a t a a t t g g a c a a a c t a c c t a c a g a g a a g a a a c a c t t c c t t g g a a t g a a g a c a c c a c a c t g t a t t a a c c t g t t t g a t g g a t g t c t c t

10,260 small t intron t t t a a a g c t c t a a g g t a a a t a t a a a a t t t t t a a g t g t a t a a t g t g t t a a a c t a c t g a t t c a a a t t t c g a g a t t c c a t t t a t a t t t t a a a a a t t c a c a t a t t a c a c a a t t t g a t g a c t a a g

10,320 small t intron t a a t t g t t t g t g t a t t t t a g a t t c c a a c c t a t g g a a c t g a t g a a t g g g a g c a g t g g t g g a a t t a a c a a a c a c a t a a a a t c t a a g g t t g g a t a c c t t g a c t a c t t a c c c t c g t c a c c a c c t

10,380 MPSSDDE a t g c c t t t a a t g a g g a a a a c c t g t t t t g c t c a g a a g a a a t g c c a t c t a g t g a t g a t g a g g t a c g g a a a t t a c t c c t t t t g g a c a a a a c g a g t c t t c t t t a c g g t a g a t c a c t a c t a c t c c

10,440 130 PKKKRKV EDP SV40 NLS ATADSQHSTPPKKKRKVEDP c t a c t g c t g a c t c t c a a c a t t c t a c t c c t c c a a a a a a g a a g a g a a a g g t a g a a g a c c c c a g a t g a c g a c t g a g a g t t g t a a g a t g a g g a g g t t t t t t c t t c t c t t t c c a t c t t c t g g g g t

10,500 (in frame with SV40 NLS) KDFPSELLSFLSHAVFSNRT KDFPSELLSFLSHAVFSNRT a g g a c t t t c c t t c a g a a t t g c t a a g t t t t t t g a g t c a t g c t g t g t t t a g t a a t a g a a c t c t c c t g a a a g g a a g t c t t a a c g a t t c a a a a a a c t c a g t a c g a c a c a a a t c a t t a t c t t g a g

10,560 (in frame with SV40 NLS) LACFAIYTTKEKAALLYKKI LACFAIYTTKEKAALLYKKI t t g c t t g c t t t g c t a t t t a c a c c a c a a a g g a a a a a g c t g c a c t g c t a t a c a a g a a a a t t a a a c g a a c g a a a c g a t a a a t g t g g t g t t t c c t t t t t c g a c g t g a c g a t a t g t t c t t t t a a t

10,620 (in frame with SV40 NLS) MEKYSVTFISRHNSYNHNIL MEKYSVTFISRHNSYNHNIL t g g a a a a a t a t t c t g t a a c c t t t a t a a g t a g g c a t a a c a g t t a t a a t c a t a a c a t a c t g t a c c t t t t t a t a a g a c a t t g g a a a t a t t c a t c c g t a t t g t c a a t a t t a g t a t t g t a t g a c a

10,680 (in frame with SV40 NLS) FFLTPHRHRVSAINNYAQKL FFLTPHRHRVSAINNYAQKL t t t t t c t t a c t c c a c a c a g g c a t a g a g t g t c t g c t a t t a a t a a c t a t g c t c a a a a a t t g t a a a a a g a a t g a g g t g t g t c c g t a t c t c a c a g a c g a t a a t t a t t g a t a c g a g t t t t t a a c a

10,740 (in frame with SV40 NLS) CTFSFLICKGVNKEYLMYSA CTFSFLICKGVNKEYLMYSA g t a c c t t t a g c t t t t t a a t t t g t a a a g g g g t t a a t a a g g a a t a t t t g a t g t a t a g t g c c t c a t g g a a a t c g a a a a a t t a a a c a t t t c c c c a a t t a t t c c t t a t a a a c t a c a t a t c a c g g a

10,800 (in frame with SV40 NLS) LTRDHNQPYHICRGFTCFKK LTRDHNQPYHICRGFTCFKK t g a c t a g a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c a c t g a t c t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g

10,860 (in frame with SV40 NLS) PPTPPPEPET* SV40 poly(A) signal PPTPPPEPET* c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c

10,920 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t

10,980 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a

11,040 SV40 poly(A) signal g t c t g g a t c a a c t g g a t a a c t c a a g c t a a c c a a a a t c a t c c c a a a c t t c c c a c c c c a t a c c a g a c c t a g t t g a c c t a t t g a g t t c g a t t g g t t t t a g t a g g g t t t g a a g g g t g g g g t a t g

11,100 c c t a t t a c c a c t g c c a a t t a c c t g t g g t t t c a t t t a c t c t a a a c c t g t g a t t c c t c t g a a g g a t a a t g g t g a c g g t t a a t g g a c a c c a a a g t a a a t g a g a t t t g g a c a c t a a g g a g a c t t

3ʹ 5ʹ 11,155 t t a t t t t c a t t t t a a a g a a a t t g t a t t t g t t a a a t a t g t a c t a c a a a c t t a g t a g a a t a a a a g t a a a a t t t c t t t a a c a t a a a c a a t t t a t a c a t g a t g t t t g a a t c a t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.