Addgene SepOTSζ - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: SepOTSζ


Map View

Eco53kI (7741) BsaBI (6883) PaqCI (6824) MscI (6155) lac operator M13/pUC Reverse (6086 .. 6108) ApaI (5291) PspOMI (5287) NmeAIII (5286) BstEII (5261) MluI (5080) LacI-R (4749 .. 4768) MauBI (4619) SacII (4107) BglI (4106) SacI (7743) NdeI (37) AgeI - SgrAI (327) FspI (337) AfeI (580) BspEI (647) BlpI (928) BstBI (961) BsrGI (973) BmgBI (1058) PshAI (1159) AscI (1235) SbfI (1252) pBAD Reverse (1298 .. 1315) BsaI (1438) NheI (1700) BmtI (1704) pGEX 3' (2054 .. 2076) PflFI - Tth111I (2174) pRS-marker (2217 .. 2236) L4440 (2319 .. 2336) PciI (2429) pBR322ori-F (2570 .. 2589) pENTR-R (3189 .. 3208) Kan-R (3273 .. 3292) EcoNI (3543) AsiSI (3631) SepOTS ζ 7750 bp

Sequence View

NdeI 5ʹ 3ʹ 60 MGSMSKE LINTLYLFSIYMHSGHRFF t a a a g t a t a t t a g t t a a g t a t a a g a a g g a g a t a t a c a t a t g g g a t c c a t g t c t a a a g a a a a t t t c a t a t a a t c a a t t c a t a t t c t t c c t c t a t a t g t a t a c c c t a g g t a c a g a t t t c t t t

120 KFERTKPHVNVGTIGHVDHG LKFTCFRVNVDTSDAVNVMT a g t t t g a a c g t a c a a a a c c g c a c g t t a a c g t c g g t a c t a t c g g c c a c g t t g a c c a t g g t a t c a a a c t t g c a t g t t t t g g c g t g c a a t t g c a g c c a t g a t a g c c g g t g c a a c t g g t a c c a t

180 KTTLTAAITTVLAKTYGGAA FCRQGSCDSGYQSFGVATSS a a a c a a c g c t g a c c g c t g c a a t c a c t a c c g t a c t g g c t a a a a c c t a c g g c g g t g c t g c t c t t t g t t g c g a c t g g c g a c g t t a g t g a t g g c a t g a c c g a t t t t g g a t g c c g c c a c g a c g a g

240 RAFDQIDNAPEEKARGITIN ACEVLDIVRRFFFSTTDGDV g c g c a t t c g a c c a g a t c g a t a a c g c g c c g g a a g a a a a a g c t c g t g g t a t c a c c a t c a a c a c g c g t a a g c t g g t c t a g c t a t t g c g c g g c c t t c t t t t t c g a g c a c c a t a g t g g t a g t t g t

300 TSRVEYDTPTRHYAHVDCPG SRPNFVVGRGTVVCVYVARP c t t c t c g g g t t g a a t a c g a c a c c c c g a c c c g t c a c t a c g c a c a c g t a g a c t g c c c g g g g c g a a g a g c c c a a c t t a t g c t g t g g g g c t g g g c a g t g a t g c g t g t g c a t c t g a c g g g c c c c g

FspISgrAIAgeI 360 HADYVKNMITGAAQMDGAIL VGVINFVHDGTSRLHVARDQ a c g c c g a c t a t g t t a a a a a c a t g a t c a c c g g t g c t g c g c a g a t g g a c g g c g c g a t c c t g g t g c g g c t g a t a c a a t t t t t g t a c t a g t g g c c a c g a c g c g t c t a c c t g c c g c g c t a g g a c c

420 VVAATDGPMPQTREHILLGR YNSRSVARHRLSTLVDQQTT t a g t t g c t g c g a c t g a c g g c c c g a t g c c g c a g a c t c g t g a g c a c a t c c t g c t g g g t c g t c a t c a a c g a c g c t g a c t g c c g g g c t a c g g c g t c t g a g c a c t c g t g t a g g a c g a c c c a g c a g

480 QVGVPYIIVFLNKCDMVDDE LYANRVDDHEQVFAVHNIVF a g g t a g g c g t t c c g t a c a t c a t c g t g t t c c t g a a c a a a t g c g a c a t g g t t g a t g a c g a a g t c c a t c c g c a a g g c a t g t a g t a g c a c a a g g a c t t g t t t a c g c t g t a c c a a c t a c t g c t t c

540 ELLELVEMEVRELLSQYDFP LQQFQNFHFNTFKQRLVVER a g c t g c t g g a a c t g g t t g a a a t g g a a g t t c g t g a a c t t c t g t c t c a g t a c g a c t t c c c g g t c g a c g a c c t t g a c c a a c t t t a c c t t c a a g c a c t t g a a g a c a g a g t c a t g c t g a a g g g c c

AfeI 600 GDDTPIVRGSALKALEGDAE AVVSRDNTTRSQFRQFAVCL g c g a c g a c a c t c c g a t c g t t c g t g g t t c t g c t c t g a a a g c g c t g g a a g g c g a c g c a g a g t c g c t g c t g t g a g g c t a g c a a g c a c c a a g a c g a g a c t t t c g c g a c c t t c c g c t g c g t c t c a

BspEI 660 WEAKILELAGFLDSYIPEPE PFRFDQFQSAEQIRVNRFWL g g g a a g c g a a a a t c c t g g a a c t g g c t g g c t t c c t g g a t t c t t a c a t t c c g g a a c c a g a g c c c c t t c g c t t t t a g g a c c t t g a c c g a c c g a a g g a c c t a a g a a t g t a a g g c c t t g g t c t c g

720 RAIDKPFLLPINGVYSISGR TRNVLREQQRDVPYVGDGTT g t g c g a t t g a c a a g c c g t t c c t g c t g c c g a t c a a c g g g g t a t a c t c c a t c t c c g g t c g t g c a c g c t a a c t g t t c g g c a a g g a c g a c g g c t a g t t g c c c c a t a t g a g g t a g a g g c c a g c a c

780 GTVVSGRVERGIIKVGEEVE TGNNRTTYFATDDFNTFFNF g t a c c g t t g t t t c g g g t c g t g t a g a a c g c g g t a t c a t c a a a g t t g g t g a a g a a g t t g a a a c a t g g c a a c a a a g c c c a g c a c a t c t t g c g c c a t a g t a g t t t c a a c c a c t t c t t c a a c t t t

840 IVGIKETQKSTCTGVEMFRK DNTDFLSLLRGTSANFHEAF t c g t t g g t a t c a a a g a g a c t c a g a a g t c t a c c t g t a c t g g c g t t g a a a t g t t c c g c a a a c a g c a a c c a t a g t t t c t c t g a g t c t t c a g a t g g a c a t g a c c g c a a c t t t a c a a g g c g t t t g

900 LLDEGRAGEWVGVLLRGIKR QQVFATSTLPYTNQQTTDFT t g c t g g a c g a a g g c c g t g c t g g t g a g t g g g t a g g t g t t c t g c t g c g t g g t a t c a a a c g t g a c g a c c t g c t t c c g g c a c g a c c a c t c a c c c a t c c a c a a g a c g a c g c a c c a t a g t t t g c a c

BlpI 960 EEIERGQVLAKPGTIKPHTK FFDFTTLYQSLRAGDLRVGL a a g a a a t c g a a c g t g g t c a g g t a c t g g c t a a g c c g g g c a c c a t c a a g c c g c a c a c c a a g t t t c t t t a g c t t g c a c c a g t c c a t g a c c g a t t c g g c c c g t g g t a g t t c g g c g t g t g g t t c a

BsrGIBstBI 1020 FESEVYILSKDEGGRHTPFF EFRFHVNQGFIFAATM t c g a a t c t g a a g t g t a c a t t c t g t c c a a a g a t g a a g g c g g c c g t c a t a c t c c g t t c t t c a a g c t t a g a c t t c a c a t g t a a g a c a g g t t t c t a c t t c c g c c g g c a g t a t g a g g c a a g a a g t

BmgBI 1080 KGYRPQFYFRTTDVTGTIEL a a g g c t a c c g t c c g c a g t t c t a c t t c c g t a c t a c t g a c g t g a c t g g t a c c a t c g a a c t g c t t c c g a t g g c a g g c g t c a a g a t g a a g g c a t g a t g a c t g c a c t g a c c a t g g t a g c t t g a c g

1140 PEGVEMVMPGDNIKMVVTLI c g g a a g g c g t a g a g a t g g t a a t g c c g g g c g a c a a c a t c a a a a t g g t t g t t a c c c t g a t c c g c c t t c c g c a t c t c t a c c a t t a c g g c c c g c t g t t g t a g t t t t a c c a a c a a t g g g a c t a g g

PshAI 1200 HPIAMDDGLRFAIREGGRTV a c c c g a t c g c g a t g g a c g a c g g t c t g c g t t t c g c a a t c c g t g a a g g c g g c c g t a c c g t t g t g g g c t a g c g c t a c c t g c t g c c a g a c g c a a a g c g t t a g g c a c t t c c g c c g g c a t g g c a a c

SbfIAscI 1260 GAGVVAKVLS* g c g c g g g c g t t g t a g c a a a a g t t c t g a g c t a a t g g c g c g c c g t c g a c c t g c a g g c a t g c a c g c g c c c g c a a c a t c g t t t t c a a g a c t c g a t t a c c g c g c g g c a g c t g g a c g t c c g t a c g t

1320 G G A C T A T G T C T A A T T T A G pBAD Reverse a g c t t g g c t g t t t t g g c g g a t g a g a g a a g a t t t t c a g c c t g a t a c a g a t t a a a t c a g a a c t c g a a c c g a c a a a a c c g c c t a c t c t c t t c t a a a a g t c g g a c t a t g t c t a a t t t a g t c t t g

1380 g c a g a a g c g g t c t g a t a a a a c a g a a t t t g c c t g g c g g c a g t a g c g c g g t g g t c c c a c c t g c g t c t t c g c c a g a c t a t t t t g t c t t a a a c g g a c c g c c g t c a t c g c g c c a c c a g g g t g g a c

BsaI 1440 a c c c c a t g c c g a a c t c a g a a g t g a a a c g c c g t a g c g c c g a t g g t a g t g t g g g g t c t c c c c t g g g g t a c g g c t t g a g t c t t c a c t t t g c g g c a t c g c g g c t a c c a t c a c a c c c c a g a g g g g

1500 rrnB T1 terminator a t g c g a g a g t a g g g a a c t g c c a g g c a t c a a a t a a a a c g a a a g g c t c a g t c g a a a g a c t g g t a c g c t c t c a t c c c t t g a c g g t c c g t a g t t t a t t t t g c t t t c c g a g t c a g c t t t c t g a c c

1560 rrnB T1 terminator g c c t t t c g t t t t a t c t g t t g t t t g t c g g t g a a c g c t c t c c t g a g t a g g a c a a a t c c g c c g c g g a a a g c a a a a t a g a c a a c a a a c a g c c a c t t g c g a g a g g a c t c a t c c t g t t t a g g c g g c

1620 g g a g c g g a t t t g a a c g t t g c g a a g c a a c g g c c c g g a g g g t g g c g g g c a g g a c g c c c g c c a c c t c g c c t a a a c t t g c a a c g c t t c g t t g c c g g g c c t c c c a c c g c c c g t c c t g c g g g c g g t

1680 rrnB T2 terminator t a a a c t g c c a g g c a t c a a a t t a a g c a g a a g g c c a t c c t g a c g g a t g g c c t t t t t g c g t t t a t t t g a c g g t c c g t a g t t t a a t t c g t c t t c c g g t a g g a c t g c c t a c c g g a a a a a c g c a a a

BmtINheI 1740 c t a c a a a c t c t t t t t g t t t g c t a g c t t t t c t c t g g t c c c g c c g c a t c c a t a c c g c c a g t t g a t g t t t g a g a a a a a c a a a c g a t c g a a a a g a g a c c a g g g c g g c g t a g g t a t g g c g g t c a a

1800 g t t t a c c c t c a c a a c g t t c c a g t a a c c g g g c a t g t t c a t c a t c a g t a a c c c g t a t c g t g a c a a a t g g g a g t g t t g c a a g g t c a t t g g c c c g t a c a a g t a g t a g t c a t t g g g c a t a g c a c t

1860 g c a t c c t c t c t c g t t t c a t c g g t a t c a t t a c c c c c a t g a a c a g a a a t c c c c c t t a c a c g g c g t a g g a g a g a g c a a a g t a g c c a t a g t a a t g g g g g t a c t t g t c t t t a g g g g g a a t g t g c c

1920 1 5 10 15 MTKQEKTALNMARFIRS rop a g g c a t c a g t g a c c a a a c a g g a a a a a a c c g c c c t t a a c a t g g c c c g c t t t a t c a g a a g c c t c c g t a g t c a c t g g t t t g t c c t t t t t t g g c g g g a a t t g t a c c g g g c g a a a t a g t c t t c g g

1980 20 25 30 35 QTLTLLEKLNELDADEQADI rop a g a c a t t a a c g c t t c t g g a g a a a c t c a a c g a g c t g g a c g c g g a t g a a c a g g c a g a c a t c t t c t g t a a t t g c g a a g a c c t c t t t g a g t t g c t c g a c c t g c g c c t a c t t g t c c g t c t g t a g a

2040 40 45 50 55 CESLHDHADELYRSCLARFG rop g t g a a t c g c t t c a c g a c c a c g c t g a t g a g c t t t a c c g c a g c t g c c t c g c g c g t t t c g g t g c a c t t a g c g a a g t g c t g g t g c g a c t a c t c g a a a t g g c g t c g a c g g a g c g c g c a a a g c c a c

2100 60 DDGENL* rop G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g t a a g t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c a t t c

2160 c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g t c g g g g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c a g c c c

Tth111IPflFI 2220 bom C G G C pRS-marker g c g c a g c c a t g a c c c a g t c a c g t a g c g a t a g c g g a g t g t a t a c t g g c t t a a c t a t g c g g c c g c g t c g g t a c t g g g t c a g t g c a t c g c t a t c g c c t c a c a t a t g a c c g a a t t g a t a c g c c g

2280 bom A T C A G A G C A G A T T G T A pRS-marker a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t a t a t g c g g t g t g a a a t a c c g c a c a g a t g c t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a t a t a c g c c a c a c t t t a t g g c g t g t c t a c g

2340 bom G A G C G A G T G A C T G A G C G A L4440 g t a a g g a g a a a a t a c c g c a t c a g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c c a t t c c t c t t t t a t g g c g t a g t c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g

2400 t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g

PciI 2460 a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c

2520 ori a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a

2580 ori T T T C T A T G G T C pBR322ori-F c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c

2640 ori C G C A A A G G G pBR322ori-F g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t

2700 ori t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c

2760 ori t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a

2820 ori c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g

2880 ori g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g

2940 ori g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t t t c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a

3000 ori g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g

3060 ori g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g

3120 ori a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c

3180 a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a a c a a t a a a a c t g t c t g c t t a c a t a a a t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t t g t t a t t t t g a c a g a c g a a t g t a t t t

3240 1 5 10 MSHIQRETSCSRPR KanR G T T C C C C A C A A T A C T C G G T A pENTR-R MSHIQRETSCSRPR c a g t a a t a c a a g g g g t g t t a t g a g c c a t a t t c a a c g g g a a a c g t c t t g c t c t a g g c c g c g g t c a t t a t g t t c c c c a c a a t a c t c g g t a t a a g t t g c c c t t t g c a g a a c g a g a t c c g g c g c

3300 15 20 25 30 LNSNMDADLYGYKWARDNVG KanR C C A T A T T T A C C C G A G C G C T A Kan-R LNSNMDADLYGYKWARDNVG a t t a a a t t c c a a c a t g g a t g c t g a t t t a t a t g g g t a t a a a t g g g c t c g c g a t a a t g t c g g t a a t t t a a g g t t g t a c c t a c g a c t a a a t a t a c c c a t a t t t a c c c g a g c g c t a t t a c a g c c

3360 35 40 45 50 QSGATIYRLYGKPDAPELFL KanR QSGATIYRLYGKPDAPELFL g c a a t c a g g t g c g a c a a t c t a t c g a t t g t a t g g g a a g c c c g a t g c g c c a g a g t t g t t t c t c g t t a g t c c a c g c t g t t a g a t a g c t a a c a t a c c c t t c g g g c t a c g c g g t c t c a a c a a a g a

3420 55 60 65 70 KHGKGSVANDVTDEMVRLNW KanR KHGKGSVANDVTDEMVRLNW g a a a c a t g g c a a a g g t a g c g t t g c c a a t g a t g t t a c a g a t g a g a t g g t c a g a c t a a a c t g c t t t g t a c c g t t t c c a t c g c a a c g g t t a c t a c a a t g t c t a c t c t a c c a g t c t g a t t t g a c

3480 75 80 85 90 LTEFMPLPTIKHFIRTPDDA KanR LTEFMPLPTIKHFIRTPDDA g c t g a c g g a a t t t a t g c c t c t t c c g a c c a t c a a g c a t t t t a t c c g t a c t c c t g a t g a t g c c g a c t g c c t t a a a t a c g g a g a a g g c t g g t a g t t c g t a a a a t a g g c a t g a g g a c t a c t a c g

3540 95 100 105 110 WLLTTAIPGKTAFQVLEEYP KanR WLLTTAIPGKTAFQVLEEYP a t g g t t a c t c a c c a c t g c g a t c c c c g g g a a a a c a g c a t t c c a g g t a t t a g a a g a a t a t c c t a c c a a t g a g t g g t g a c g c t a g g g g c c c t t t t g t c g t a a g g t c c a t a a t c t t c t t a t a g g

EcoNI 3600 115 120 125 130 DSGENIVDALAVFLRRLHSI KanR DSGENIVDALAVFLRRLHSI t g a t t c a g g t g a a a a t a t t g t t g a t g c g c t g g c a g t g t t c c t g c g c c g g t t g c a t t c g a t a c t a a g t c c a c t t t t a t a a c a a c t a c g c g a c c g t c a c a a g g a c g c g g c c a a c g t a a g c t a

AsiSI 3660 135 140 145 150 PVCNCPFNSDRVFRLAQAQS KanR PVCNCPFNSDRVFRLAQAQS t c c t g t t t g t a a t t g t c c t t t t a a c a g c g a t c g c g t a t t t c g t c t c g c t c a g g c g c a a t c a g g a c a a a c a t t a a c a g g a a a a t t g t c g c t a g c g c a t a a a g c a g a g c g a g t c c g c g t t a g

3720 155 160 165 170 RMNNGLVDASDFDDERNGWP KanR RMNNGLVDASDFDDERNGWP a c g a a t g a a t a a c g g t t t g g t t g a t g c g a g t g a t t t t g a t g a c g a g c g t a a t g g c t g g c c t g c t t a c t t a t t g c c a a a c c a a c t a c g c t c a c t a a a a c t a c t g c t c g c a t t a c c g a c c g g

3780 175 180 185 190 VEQVWKEMHKLLPFSPDSVV KanR VEQVWKEMHKLLPFSPDSVV t g t t g a a c a a g t c t g g a a a g a a a t g c a t a a a c t t t t g c c a t t c t c a c c g g a t t c a g t c g t a c a a c t t g t t c a g a c c t t t c t t t a c g t a t t t g a a a a c g g t a a g a g t g g c c t a a g t c a g c a

3840 195 200 205 210 THGDFSLDNLIFDEGKLIGC KanR THGDFSLDNLIFDEGKLIGC c a c t c a t g g t g a t t t c t c a c t t g a t a a c c t t a t t t t t g a c g a g g g g a a a t t a a t a g g t t g g t g a g t a c c a c t a a a g a g t g a a c t a t t g g a a t a a a a a c t g c t c c c c t t t a a t t a t c c a a c

3900 215 220 225 230 IDVGRVGIADRYQDLAILWN KanR IDVGRVGIADRYQDLAILWN t a t t g a t g t t g g a c g a g t c g g a a t c g c a g a c c g a t a c c a g g a t c t t g c c a t c c t a t g g a a a t a a c t a c a a c c t g c t c a g c c t t a g c g t c t g g c t a t g g t c c t a g a a c g g t a g g a t a c c t t

3960 235 240 245 250 CLGEFSPSLQKRLFQKYGID KanR CLGEFSPSLQKRLFQKYGID c t g c c t c g g t g a g t t t t c t c c t t c a t t a c a g a a a c g g c t t t t t c a a a a a t a t g g t a t t g a g a c g g a g c c a c t c a a a a g a g g a a g t a a t g t c t t t g c c g a a a a a g t t t t t a t a c c a t a a c t

4020 255 260 265 270 NPDMSKLQFHLMLDEFF* KanR NPDMSKLQFHLMLDEFF* t a a t c c t g a t a t g a g t a a a t t g c a g t t t c a t t t g a t g c t c g a t g a g t t t t t c t a a g a a t t a t t a g g a c t a t a c t c a t t t a a c g t c a a a g t a a a c t a c g a g c t a c t c a a a a a g a t t c t t a a

4080 a a t t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c t t a a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g

BglISacII 4140 g c a c a t t t c c c c g a a a a g t g c c a c c g c g g c c g c a a a a a a a a t c c t t a g c t t t c g c t a a g g c g t g t a a a g g g g c t t t t c a c g g t g g c g c c g g c g t t t t t t t t a g g a a t c g a a a g c g a t t c c

4200 a t c t g c a g g t c g a c t c t a g a g g a t c c t g g a g c c g g g g g t g g g a t t t g a a c c c a c g t a a g g t a g a c g t c c a g c t g a g a t c t c c t a g g a c c t c g g c c c c c a c c c t a a a c t t g g g t g c a t t c c

4260 c g g a t c t a g a g t c c g c t g c c t a a c c c c t a g a c t a c c c c g g c g a a t t c a g c g t t a c a a g t a g c c t a g a t c t c a g g c g a c g g a t t g g g g a t c t g a t g g g g c c g c t t a a g t c g c a a t g t t c a t

4320 t t a c a c a a a g t t t t t t a t g t t g a g a a t a t t t t t t t g a t g g g g c g c c a c t t a t t t t t g a t c a a t g t g t t t c a a a a a a t a c a a c t c t t a t a a a a a a a c t a c c c c g c g g t g a a t a a a a a c t a g

4380 g t t c g c t c a a a g a a g c g c g g c c g c a a a a a a a a t c c t t a g c t t t c g c t a a g g a t c t g c a g g c a a g c g a g t t t c t t c g c g c c g g c g t t t t t t t t a g g a a t c g a a a g c g a t t c c t a g a c g t c c

4440 t c g a c t c t a g a g g a t c c t g g a g c c g g g g g t g g g a t t t g a a c c c a c g t a a g g c g g a t c t a g a g c t g a g a t c t c c t a g g a c c t c g g c c c c c a c c c t a a a c t t g g g t g c a t t c c g c c t a g a t c

4500 a g t c c g c t g c c t a a c c c c t a g a c t a c c c c g g c g a a t t c a g c g t t a c a a g t a t t a c a c a a a t c a g g c g a c g g a t t g g g g a t c t g a t g g g g c c g c t t a a g t c g c a a t g t t c a t a a t g t g t t t

4560 g t t t t t t a t g t t g a g a a t a t t t t t t t g a t g g g g c g c c a c t t a t t t t t g a t c g t t c g c t c a c a a a a a a t a c a a c t c t t a t a a a a a a a c t a c c c c g c g g t g a a t a a a a a c t a g c a a g c g a g t

MauBI 4620 a a g a a g c g c g g c c g c t t t c a c c g t c a t c a c c g a a a c g c g c g a g g c a g c a g a t c a a t t c g c t t c t t c g c g c c g g c g a a a g t g g c a g t a g t g g c t t t g c g c g c t c c g t c g t c t a g t t a a g c g

4680 lacIq promoter g c g c g a a g g c g a a g c g g c a t g c a t t t a c g t t g a c a c c a t c g a a t g g t g c a a a a c c t t t c g c g c g c t t c c g c t t c g c c g t a c g t a a a t g c a a c t g t g g t a g c t t a c c a c g t t t t g g a a a g c

4740 1 MKPV lacIq promoter lacI c g g t a t g g c a t g a t a g c g c c c g g a a g a g a g t c a a t t c a g g g t g g t g a a t g t g a a a c c a g t g c c a t a c c g t a c t a t c g c g g g c c t t c t c t c a g t t a a g t c c c a c c a c t t a c a c t t t g g t c a

4800 5 10 15 20 TLYDVAEYAGVSYQTVSRVV lacI T G C T A C A G C G T C T C A T A C G G LacI-R a a c g t t a t a c g a t g t c g c a g a g t a t g c c g g t g t c t c t t a t c a g a c c g t t t c c c g c g t g g t t t g c a a t a t g c t a c a g c g t c t c a t a c g g c c a c a g a g a a t a g t c t g g c a a a g g g c g c a c c a

4860 25 30 35 40 NQASHVSAKTREKVEAAMAE lacI MAE g a a c c a g g c c a g c c a c g t t t c t g c g a a a a c g c g g g a a a a a g t g g a a g c g g c g a t g g c g g a c t t g g t c c g g t c g g t g c a a a g a c g c t t t t g c g c c c t t t t t c a c c t t c g c c g c t a c c g c c t

4920 45 50 55 60 LNYIPNRVAQQLAGKQSLLI lacI LNYIPNRVAQQLAGKQSLLI g c t g a a t t a c a t t c c c a a c c g c g t g g c a c a a c a a c t g g c g g g c a a a c a g t c g t t g c t g a t c g a c t t a a t g t a a g g g t t g g c g c a c c g t g t t g t t g a c c g c c c g t t t g t c a g c a a c g a c t a

4980 65 70 75 80 GVATSSLALHAPSQIVAAIK lacI GVATSSLALHAPSQIVAAIK t g g c g t t g c c a c c t c c a g t c t g g c c c t g c a c g c g c c g t c g c a a a t t g t c g c g g c g a t t a a a c c g c a a c g g t g g a g g t c a g a c c g g g a c g t g c g c g g c a g c g t t t a a c a g c g c c g c t a a t t

5040 85 90 95 100 SRADQLGASVVVSMVERSGV lacI SRADQLGASVVVSMVERSGV a t c t c g c g c c g a t c a a c t g g g t g c c a g c g t g g t g g t g t c g a t g g t a g a a c g a a g c g g c g t t a g a g c g c g g c t a g t t g a c c c a c g g t c g c a c c a c c a c a g c t a c c a t c t t g c t t c g c c g c a

MluI 5100 105 110 115 120 EACKAAVHNLLAQRVSGLII lacI EACKAAVHNLLAQRVSGLII c g a a g c c t g t a a a g c g g c g g t g c a c a a t c t t c t c g c g c a a c g c g t c a g t g g g c t g a t c a t g c t t c g g a c a t t t c g c c g c c a c g t g t t a g a a g a g c g c g t t g c g c a g t c a c c c g a c t a g t a

5160 125 130 135 140 NYPLDDQDAIAVEAACTNVP lacI NYPLDDQDAIAVEAACTNVP t a a c t a t c c g c t g g a t g a c c a g g a t g c c a t t g c t g t g g a a g c t g c c t g c a c t a a t g t t c c a t t g a t a g g c g a c c t a c t g g t c c t a c g g t a a c g a c a c c t t c g a c g g a c g t g a t t a c a a g g

5220 145 150 155 160 ALFLDDSDQTPINSIIFSHE lacI ALFLDDSDQTPINSIIFSHE g g c g t t a t t t c t t g a t g a c t c t g a c c a g a c a c c c a t c a a c a g t a t t a t t t t c t c c c a t g a c c g c a a t a a a g a a c t a c t g a g a c t g g t c t g t g g g t a g t t g t c a t a a t a a a a g a g g g t a c t

BstEII 5280 165 170 175 180 DGTRLGVEHLVALGHQQIAL lacI DGTRLGVEHLVALGHQQIAL a g a c g g t a c g c g a c t g g g c g t g g a g c a t c t g g t c g c a t t g g g t c a c c a g c a a a t c g c g c t t c t g c c a t g c g c t g a c c c g c a c c t c g t a g a c c a g c g t a a c c c a g t g g t c g t t t a g c g c g a

NmeAIIIPspOMIApaI 5340 185 190 195 200 LAGPLSSVSARLRLAGWHKY lacI LAGPLSSVSARLRLAGWHKY g t t a g c g g g c c c a t t a a g t t c t g t c t c g g c g c g t c t g c g t c t g g c t g g c t g g c a t a a a t a c a a t c g c c c g g g t a a t t c a a g a c a g a g c c g c g c a g a c g c a g a c c g a c c g a c c g t a t t t a t

5400 205 210 215 220 LTRNQIQPIAEREGDWSAMS lacI LTRNQIQPIAEREGDWSAMS t c t c a c t c g c a a t c a a a t t c a g c c g a t a g c g g a a c g g g a a g g c g a c t g g a g t g c c a t g t c a g a g t g a g c g t t a g t t t a a g t c g g c t a t c g c c t t g c c c t t c c g c t g a c c t c a c g g t a c a g

5460 225 230 235 240 GFQQTMQMLNEGIVPTAMLV lacI GFQQTMQMLNEGIVPTAMLV c g g t t t t c a a c a a a c c a t g c a a a t g c t g a a t g a g g g c a t c g t t c c c a c t g c g a t g c t g g t g c c a a a a g t t g t t t g g t a c g t t t a c g a c t t a c t c c c g t a g c a a g g g t g a c g c t a c g a c c a

5520 245 250 255 260 ANDQMALGAMRAITESGLRV lacI ANDQMALGAMRAITESGLRV t g c c a a c g a t c a g a t g g c g c t g g g c g c a a t g c g c g c c a t t a c c g a g t c c g g g c t g c g c g t a c g g t t g c t a g t c t a c c g c g a c c c g c g t t a c g c g c g g t a a t g g c t c a g g c c c g a c g c g c a

5580 265 270 275 280 GADISVVGYDDTEDSSCYIP lacI GADISVVGYDDTEDSSCYIP t g g t g c g g a t a t c t c g g t a g t g g g a t a c g a c g a t a c c g a a g a c a g c t c a t g t t a t a t c c c a c c a c g c c t a t a g a g c c a t c a c c c t a t g c t g c t a t g g c t t c t g t c g a g t a c a a t a t a g g g

5640 285 290 295 300 PLTTIKQDFRLLGQTSVDRL lacI PLTTIKQDFRLLGQTSVDRL g c c g t t a a c c a c c a t c a a a c a g g a t t t t c g c c t g c t g g g g c a a a c c a g c g t g g a c c g c t t c g g c a a t t g g t g g t a g t t t g t c c t a a a a g c g g a c g a c c c c g t t t g g t c g c a c c t g g c g a a

5700 305 310 315 320 LQLSQGQAVKGNQLLPVSLV lacI LQLSQGQAVKGNQLLPVSLV g c t g c a a c t c t c t c a g g g c c a g g c g g t g a a g g g c a a t c a g c t g t t g c c c g t c t c a c t g g t c g a c g t t g a g a g a g t c c c g g t c c g c c a c t t c c c g t t a g t c g a c a a c g g g c a g a g t g a c c a

5760 325 330 335 340 KRKTTLAPNTQTASPRALAD lacI KRKTTLAPNTQTASPRALAD g a a a a g a a a a a c c a c c c t g g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a c t t t t c t t t t t g g t g g g a c c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t

5820 345 350 355 360 SLMQLARQVSRLESGQ* lacI SLMQLARQVSRLESGQ* t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c

5880 c a a t t a a t g t g a g t t a g c g c g a a t t g a t c t g g t t t g a c a g c t t a t c a t c g a c t g c a c g g t g t t a a t t a c a c t c a a t c g c g c t t a a c t a g a c c a a a c t g t c g a a t a g t a g c t g a c g t g c c a

5940 g c a c c a a t g c t t c t g g c g t c a g g c a g c c a t c g g a a g c t g t g g t a t g g c t g t g c a g g t c g t c g t g g t t a c g a a g a c c g c a g t c c g t c g g t a g c c t t c g a c a c c a t a c c g a c a c g t c c a g c a

6000 a a a t c a c t g c a t a a t t c g t g t c g c t c a a g g c g c a c t c c c g t t c t g g a t a a t g t t t t t t g c t t t a g t g a c g t a t t a a g c a c a g c g a g t t c c g c g t g a g g g c a a g a c c t a t t a c a a a a a a c g

6060 -35 trc promoter g c c g a c a t c a t a a c g g t t c t g g c a a a t a t t c t g a a a t g a g c t g t t g a c a a t t a a t c a t c c c g g c t g t a g t a t t g c c a a g a c c g t t t a t a a g a c t t t a c t c g a c a a c t g t t a a t t a g t a g g

6120 -10 trc promoter lac operator A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse M g g c t c g t a t a a t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g a c c c a t c c g a g c a t a t t a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c t g g g t a

MscI 6180 AMFKREEIIEMANKDFEKAW g g c a a t g t t t a a a a g a g a a g a a a t c a t t g a a a t g g c c a a t a a g g a c t t t g a a a a a g c a t g c c g t t a c a a a t t t t c t c t t c t t t a g t a a c t t t a c c g g t t a t t c c t g a a a c t t t t t c g t a c

6240 IETKDLIKAKKINESYPRIK g a t c g a a a c t a a a g a c c t t a t a a a a g c t a a a a a g a t a a a c g a a a g t t a c c c a a g a a t a a a c t a g c t t t g a t t t c t g g a a t a t t t t c g a t t t t t c t a t t t g c t t t c a a t g g g t t c t t a t t t

6300 PVFGKTHPVNDTIENLRQAY a c c a g t t t t t g g a a a a a c a c a c c c t g t a a a t g a c a c t a t t g a a a a t t t a a g a c a g g c a t a t g g t c a a a a a c c t t t t t g t g t g g g a c a t t t a c t g t g a t a a c t t t t a a a t t c t g t c c g t a t

6360 LRMGFEEYINPVIVDERDIY t c t t a g a a t g g g t t t t g a a g a a t a t a t a a a c c c a g t a a t t g t c g a t g a a a g a g a t a t t t a a g a a t c t t a c c c a a a a c t t c t t a t a t a t t t g g g t c a t t a a c a g c t a c t t t c t c t a t a a a t

6420 KQFGPEAMAVLDRCFYLAGL t a a a c a a t t c g g c c c a g a a g c t a t g g c a g t t t t g g a t a g a t g c t t t t a t t t a g c g g g a c t a t t t g t t a a g c c g g g t c t t c g a t a c c g t c a a a a c c t a t c t a c g a a a a t a a a t c g c c c t g a

6480 PRPDVGLSDEKISQIEKLGI t c c a a g a c c t g a c g t t g g t t t g a g c g a t g a a a a a a t t t c a c a g a t t g a a a a a c t t g g a a t a g g t t c t g g a c t g c a a c c a a a c t c g c t a c t t t t t t a a a g t g t c t a a c t t t t t g a a c c t t a

6540 KVSEHKESLQKILHGYKKGT t a a a g t t t c t g a g c a c a a a g a a a g t t t a c a a a a a a t a c t t c a c g g a t a c a a a a a a g g a a c a t t t c a a a g a c t c g t g t t t c t t t c a a a t g t t t t t t a t g a a g t g c c t a t g t t t t t t c c t t g

6600 LDGDDLVLEISNALEISSEM t c t t g a t g g t g a c g a t t t a g t t t t a g a a a t t t c a a a t g c a c t t g a a a t t t c a a g c g a g a t a g a a c t a c c a c t g c t a a a t c a a a a t c t t t a a a g t t t a c g t g a a c t t t a a a g t t c g c t c t a

6660 GLKILEDVFPEFKDLTAVSS g g g t t t a a a a a t t t t a g a a g a t g t t t t c c c a g a a t t t a a g g a t t t a a c c g c a g t t t c t t c c c c a a a t t t t t a a a a t c t t c t a c a a a a g g g t c t t a a a t t c c t a a a t t g g c g t c a a a g a a g

6720 KLTLRSHMTSGWFLTVSDLM a a a a t t a a c t t t a a g a a g c c a c a t g a c t t c a g g a t g g t t c c t t a c t g t t t c a g a c c t c a t t t t t a a t t g a a a t t c t t c g g t g t a c t g a a g t c c t a c c a a g g a a t g a c a a a g t c t g g a g t a

6780 NKKPLPFKLFSIDRCFRREQ g a a c a a a a a a c c c t t g c c a t t t a a a c t c t t t t c a a t c g a t a g a t g t t t t a g a a g a g a a c a c t t g t t t t t t g g g a a c g g t a a a t t t g a g a a a a g t t a g c t a t c t a c a a a a t c t t c t c t t g t

PaqCI 6840 KEDKSHLMTYHSASCAIAGE a a a a g a a g a t a a a a g c c a c t t a a t g a c a t a c c a c t c t g c a t c c t g t g c a a t t g c a g g t g a t t t t c t t c t a t t t t c g g t g a a t t a c t g t a t g g t g a g a c g t a g g a c a c g t t a a c g t c c a c t

BsaBI 6900 GVDINDGKAIAEGLLSQFGF a g g c g t g g a t a t t a a t g a t g g a a a a g c a a t t g c a g a a g g a t t a t t a t c c c a a t t t g g c t t t c c g c a c c t a t a a t t a c t a c c t t t t c g t t a a c g t c t t c c t a a t a a t a g g g t t a a a c c g a a

6960 TNFKFIPDEKKSKYYTPETQ t a c a a a c t t t a a a t t c a t t c c t g a t g a a a a g a a a a g t a a a t a c t a c a c c c c t g a a a c a c a a t g t t t g a a a t t t a a g t a a g g a c t a c t t t t c t t t t c a t t t a t g a t g t g g g g a c t t t g t g t

7020 TEVYAYHPKLKEWLEVATFG g a c t g a a g t t t a c g c a t a c c a c c c a a a a t t a a a a g a a t g g c t c g a a g t t g c t a c a t t t g g c t g a c t t c a a a t g c g t a t g g t g g g t t t t a a t t t t c t t a c c g a g c t t c a a c g a t g t a a a c c

7080 VYSPVALSKYGIDVPVMNLG a g t a t a t t c g c c a g t t g c a t t a a g c a a a t a c g g a a t a g a t g t a c c t g t a a t g a a t t t g g g t c a t a t a a g c g g t c a a c g t a a t t c g t t t a t g c c t t a t c t a c a t g g a c a t t a c t t a a a c c c

7140 LGVERLAMISGNFADVREMV t c t t g g t g t t g a a a g a c t t g c a a t g a t t t c t g g a a a t t t c g c a g a t g t t c g a g a a a t g g t a g a a c c a c a a c t t t c t g a a c g t t a c t a a a g a c c t t t a a a g c g t c t a c a a g c t c t t t a c c a

7200 YPQFYEHKLNDRNVASMVKL a t a t c c t c a g t t t t a c g a a c a c a a a c t t a a t g a c c g g a a t g t c g c t t c a a t g g t a a a a c t t a t a g g a g t c a a a a t g c t t g t g t t t g a a t t a c t g g c c t t a c a g c g a a g t t a c c a t t t t g a

7260 DKVPVMDEIYDLTKELIESC c g a t a a a g t t c c a g t a a t g g a t g a a a t t t a c g a t t t a a c a a a a g a a t t a a t t g a g t c a t g g c t a t t t c a a g g t c a t t a c c t a c t t t a a a t g c t a a a t t g t t t t c t t a a t t a a c t c a g t a c

7320 VKNKDLKSPCELAIEKTFSF t g t t a a a a a c a a a g a t t t a a a a t c c c c t t g t g a a t t a g c t a t t g a a a a a a c g t t t t c a t t a c a a t t t t t g t t t c t a a a t t t t a g g g g a a c a c t t a a t c g a t a a c t t t t t t g c a a a a g t a a

7380 GKTKKNVKINIFEKEEGKNL t g g a a a a a c c a a g a a a a a t g t a a a a a t a a a c a t t t t t g a a a a a g a a g a a g g t a a a a a t t t a c c t t t t t g g t t c t t t t t a c a t t t t t a t t t g t a a a a a c t t t t t c t t c t t c c a t t t t t a a a

7440 LGPSILNEIYVYDGNVIGIP *SPFTIPIG a c t c g g a c c t t c a a t t t t a a a c g a a a t c t a c g t t t a c g a t g g a a a t g t a a t t g g a a t t c c t g a g c c t g g a a g t t a a a a t t t g c t t t a g a t g c a a a t g c t a c c t t t a c a t t a a c c t t a a g g

7500 ESFDGVKEEFKDFLEKGKSE SLKSPTFSSNLSKKSFPFDS t g a a a g c t t t g a c g g a g t a a a a g a a g a a t t t a a a g a c t t c t t a g a a a a a g g a a a a t c a g a a c t t t c g a a a c t g c c t c a t t t t c t t c t t a a a t t t c t g a a g a a t c t t t t t c c t t t t a g t c t

7560 GVATGIRYIDALCFKITSKL PTAVPMRYISASQKLIVEFN a g g g g t a g c a a c a g g c a t t c g a t a t a t c g a t g c g c t t t g c t t t a a a a t t a c t t c a a a a t t t c c c c a t c g t t g t c c g t a a g c t a t a t a g c t a c g c g a a a c g a a a t t t t a a t g a a g t t t t a a

7620 EEAFVSNTTEFKVKVPIVRS SSANTDFVVSNLTLTGITLL a g a a g a a g c a t t t g t g t c a a a c a c t a c t g a a t t c a a a g t t a a a g t t c c a a t t g t c a g a a g t c t t c t t c g t a a a c a c a g t t t g t g a t g a c t t a a g t t t c a a t t t c a a g g t t a a c a g t c t t c

7680 LSDINLKIDDIALKQIMSKN KLSMLKFISSIANFCIMLLF t t t a a g c g a c a t t a a c t t a a a a a t c g a t g a t a t c g c a t t a a a a c a g a t c a t g a g c a a a a a a a a t t c g c t g t a a t t g a a t t t t t a g c t a c t a t a g c g t a a t t t t g t c t a g t a c t c g t t t t t

7740 KVIDVRGPVFLNVEVKIE* LTISTLPGTKKFTSTFISYS t a a a g t a a t c g a c g t t a g a g g c c c a g t c t t t t t a a a t g t c g a a g t a a a a a t t g a a t a a g a a t t t c a t t a g c t g c a a t c t c c g g g t c a g a a a a a t t t a c a g c t t c a t t t t t a a c t t a t t c t

Eco53kISacI 3ʹ 5ʹ 7750 SPV g c t c g g t a c c c g a g c c a t g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.