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Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation.

Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, Liu Z, Mancini MA, Li W
Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
177120plenti-ef1a-dCasRx-FTO-HA-T2A-BSDdCasRx mediated RNA tethering of FTO
177121pTXBI-mclover-YTHDC1-FLBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
177122pTXBI-mclover-YTH-IDR2Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
177123pTXBI-mClover-YTH domainBacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
177124pHR-YTHDC1-FL-mCherry-Cry2Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay
177125pHR-IDR1-mCherry-Cry2Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay
177126pHR-YTH-mCherry-Cry2Express YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay
177130pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-ResistantGenerate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

Antibodies from Article