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A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability.

Wilke G, Funkhouser-Jones LJ, Wang Y, Ravindran S, Wang Q, Beatty WL, Baldridge MT, VanDussen KL, Shen B, Kuhlenschmidt MS, Kuhlenschmidt TB, Witola WH, Stappenbeck TS, Sibley LD
Cell Host Microbe. 2019 Jun 18. pii: S1931-3128(19)30252-5. doi: 10.1016/j.chom.2019.05.007. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
122852pACT1:Cas9-GFP, U6:sgTKExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene
122853pACT1:Cas9-GFP, U6:sgUPRTExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene
134896TK-GFP-Nluc-P2A-neo-TKExpresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus
135015UPRT-mCh-Nluc-P2A-neo-UPRTExpresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

Antibodies from Article