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Phosphoinositide Interactions Position cGAS at the Plasma Membrane to Ensure Efficient Distinction between Self- and Viral DNA.
Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch PJ, Kagan JC
Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 10.1016/j.cell.2019.01.049. Epub 2019 Feb 28.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
130908pRRL-gRNA(anti-cGAS)-Cas9-PurogRNA targeting human cGAS; Cas9 insert; Puromycin selection marker
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130910pLenti-CMV-cGAS-HAExpresses human cGAS-HA; Puromycin selection marker
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130912pLenti-CMV-cGASΔN-HAExpresses human cGASΔN (aa160-522)-HA; Puromycin selection marker
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130913pLenti-CMV-cGAS-N-HAExpresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker
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130915pLenti-CMV-cGASΔN-HA (murine)Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker
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130916pLenti-CMV-cGAS-N-HA (murine)Expresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection marker
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130917pLenti-CMV-cGAS C396/7A-HAExpresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker
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130918pLenti-CMV-cGASΔN C396/7A-HAExpresses human cGASΔN (aa160-522)-HA with C396/397A point mutation; Puromycin selection marker
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130919pLenti-CMV-cGAS R71/75E-HAExpresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker
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130920pLenti-CMV-cGAS R71/75E C396/7A-HAExpresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker
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130921pLenti-CMV-TIRAP-cGASΔN-HAExpresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker
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130922pLenti-CMV-Fyn-cGASΔN-HAExpresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker
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Antibodies from Article