Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome.
Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP
Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121966 | pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE | Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB. |
| 121968 | pHR-BRD4ΔN-mCherry-sspB | Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID. |
| 122151 | pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE | Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP. |
| 122436 | pHR-pSFFV-TAF15N-mCherry-sspB | Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID. |
| 122439 | pHR-BRD4ΔN-miRFP670-Cry2WT | Expresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT |
| 122440 | pHR-TAF15N-miRFP670-Cry2WT | Expresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT |
| 122441 | pHR-FUSN-miRFP670-Cry2WT | Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT |
| 122442 | pHR-HP1α-miRFP670-Cry2WT | Expresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT |
| 122659 | pLV-sgTel | Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker |