Systematic discovery of natural CRISPR-Cas12a inhibitors.
Watters KE, Fellmann C, Bai HB, Ren SM, Doudna JA
Science. 2018 Oct 12;362(6411):236-239. doi: 10.1126/science.aau5138. Epub 2018 Sep 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115653 | pKEW106-MbCas12a-58069 | Expresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR control |
| 115654 | pKEW107-MbCas12a-33362 | Expresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR control |
| 115656 | pKEW302-AcrVA4 | Expresses AcrVA4 in bacteria, under TetR control. |
| 115657 | pKEW303-AcrVA5 | Expresses AcrVA5 in bacteria, under TetR control. |
| 115658 | pKEW212-MBP-TEV-AcrVA4 | Expresses AcrVA4 in bacteria via T7 RNAP, for purification |
| 115660 | pKEW189-MBP-TEV-AcrVA1 | Expresses AcrVA1 in bacteria via T7 RNAP, for purification |
| 115661 | pKEW321-MbCas12a-RFP-GFP-array | Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1 |
| 115662 | pCF525-EF1a-Hygro-P2A-AcrVA1-lenti | Expresses AcrVA1 in human cells, contained in a lentiviral vector. |
| 115663 | pCF525-EF1a-Hygro-P2A-AcrVA4-lenti | Expresses AcrVA4 in human cells, contained in a lentiviral vector. |
| 115664 | pCF525-EF1a-Hygro-P2A-AcrVA5-lenti | Expresses AcrVA5 in human cells, contained in a lentiviral vector. |
| 115669 | pKEW270-pTet-empty | Vector for anti-CRISPR expression with pTet |
| 115670 | pKEW336-MBP-TEV-MbCas12a-33362 | Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification |
| 115795 | pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti | Expresses AcrIIA4 in human cells, contained in a lentiviral vector. |
| 115796 | pCF525-EF1a-Hygro-P2A-mCherry-lenti | Expresses mCherry in human cells, contained in a lentiviral vector. |
| 115797 | pCF525-EF1a-Hygro-P2A-mTagBFP2-lenti | Expresses mTagBFP in human cells, contained in a lentiviral vector. |