Addgene: Systematic discovery of natural CRISPR-Cas12a inhibitors.

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Jennifer Doudna
Watters et al

Systematic discovery of natural CRISPR-Cas12a inhibitors.
Watters KE, Fellmann C, Bai HB, Ren SM, Doudna JA
Science. 2018 Oct 12;362(6411):236-239. doi: 10.1126/science.aau5138. Epub 2018 Sep 6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
115653	pKEW106-MbCas12a-58069	Expresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR control
115654	pKEW107-MbCas12a-33362	Expresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR control
115656	pKEW302-AcrVA4	Expresses AcrVA4 in bacteria, under TetR control.
115657	pKEW303-AcrVA5	Expresses AcrVA5 in bacteria, under TetR control.
115658	pKEW212-MBP-TEV-AcrVA4	Expresses AcrVA4 in bacteria via T7 RNAP, for purification
115660	pKEW189-MBP-TEV-AcrVA1	Expresses AcrVA1 in bacteria via T7 RNAP, for purification
115661	pKEW321-MbCas12a-RFP-GFP-array	Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1
115662	pCF525-EF1a-Hygro-P2A-AcrVA1-lenti	Expresses AcrVA1 in human cells, contained in a lentiviral vector.
115663	pCF525-EF1a-Hygro-P2A-AcrVA4-lenti	Expresses AcrVA4 in human cells, contained in a lentiviral vector.
115664	pCF525-EF1a-Hygro-P2A-AcrVA5-lenti	Expresses AcrVA5 in human cells, contained in a lentiviral vector.
115669	pKEW270-pTet-empty	Vector for anti-CRISPR expression with pTet
115670	pKEW336-MBP-TEV-MbCas12a-33362	Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification
115795	pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti	Expresses AcrIIA4 in human cells, contained in a lentiviral vector.
115796	pCF525-EF1a-Hygro-P2A-mCherry-lenti	Expresses mCherry in human cells, contained in a lentiviral vector.
115797	pCF525-EF1a-Hygro-P2A-mTagBFP2-lenti	Expresses mTagBFP in human cells, contained in a lentiviral vector.

Antibodies from Article