Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6.
Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F
Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15. PubMed Article
Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F
Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115207 | pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI | Expresses BzoCas13b from a SUMO-tagged bacterial expression construct |
| 115208 | pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI | Expresses PinCas13b from a SUMO-tagged bacterial expression construct |
| 115209 | pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI | Expresses PbuCas13b from a SUMO-tagged bacterial expression construct |
| 115210 | pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI | Expresses AspCas13b from a SUMO-tagged bacterial expression construct |
| 115211 | pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI | Expresses PsmCas13b from a SUMO-tagged bacterial expression construct |
| 115213 | pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI | Expresses RanCas13b from a SUMO-tagged bacterial expression construct |
| 115214 | pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI | Expresses PauCas13b from a SUMO-tagged bacterial expression construct |
| 115216 | pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI | Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct |
| 115218 | pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI | Expresses PguCas13b from a SUMO-tagged bacterial expression construct |
| 115219 | pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI | Expresses PspCas13b from a SUMO-tagged bacterial expression construct |
| 115220 | pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI | Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct |
| 115267 | pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI | Expresses LbuCas13a from a SUMO-tagged bacterial expression construct |
| 115268 | pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI | Expresses EiCsm6 from a SUMO-tagged bacterial expression construct |
| 115270 | pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI | Expresses TtCsm6 from a SUMO-tagged bacterial expression construct |
| 182687 | pR0306 CcaCas13b His6-TwinStrep-SUMO | Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification |