Addgene: Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut.

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Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut.
Lim B, Zimmermann M, Barry NA, Goodman AL
Cell. 2017 Apr 20;169(3):547-558.e15. doi: 10.1016/j.cell.2017.03.045. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
90317	pNBU2_erm_P1T_DP-GH023	Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023
90318	pNBU2_erm_P1T_DP-A21	Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS A21
90319	pNBU2_erm_P1T_DP-B1	Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B1
90320	pNBU2_erm_P1T_DP-rpiL*	Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS rpiL*
90321	pNBU2_erm_P1T_DP-B41	Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B41
90324	pNBU2_erm-TetR-P1T_DP-GH023	Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter
90325	pExchange_tdk_BT1311p-tetR-1	Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702
90326	pExchange_tdk_BT1311p-tetR-2	Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694
90327	pExchange_tdk_BT1311p-tetR-3	Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957
117727	pNBU2_erm_P1T_DP-GH023 - NanoLuc	Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter
117728	pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc	Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Antibodies from Article