An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting.

Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q
Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
85400	Lenti-iCas9-neo	Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
85401	Lenti-multi-Guide	Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
85402	Lenti-multi-CRISPR	Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression
85745	Lenti-entry-puro	Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.