Compositional Control of Phase-Separated Cellular Bodies.
Banani SF, Rice AM, Peeples WB, Lin Y, Jain S, Parker R, Rosen MK
Cell. 2016 Jul 28;166(3):651-663. doi: 10.1016/j.cell.2016.06.010. Epub 2016 Jun 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122026 | RFP-(SUMO)10-(SIM)6 | Mammalian expression plasmid for multivalent scaffold of (SUMO)10-(SIM)6 biomolecular condensates to recruit SIM-containing client proteins. |
| 122027 | RFP-(SUMO)6-(SIM)10 | Mammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins. |
| 126866 | (SUMO)10-(SIM)5 | Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins. |
| 126923 | (SUMO)5-(SIM)10 | Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins. |
| 126946 | (SIM)10 | Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds. |
| 126947 | (SIM)5 | Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds. |
| 126948 | (SUMO)10 | Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds. |
| 126949 | (SUMO)5 | Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds. |
| 126954 | GFP-(SIM)1 | Bacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds. |
| 126955 | GFP-(SIM)3 | Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds. |
| 127089 | GFP-(SUMO)1 | Bacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds. |
| 127093 | GFP-(SUMO)3 | Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds. |
| 127094 | RFP-(SIM)1 | Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds. |
| 127148 | RFP-(SIM)3 | Mammalian expression plasmid for mCherry-(SIM)3 client to assess SUMO-SIM scaffolds. |
| 127150 | RFP-(SUMO)3 | Mammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds. |
| 130703 | RFP-(SUMO FAAA)3 | Mammalian expression plasmid for negative control of mCherry-(SUMO)3. |