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In vivo genome editing using Staphylococcus aureus Cas9.
Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
Nature. 2015 Apr 1. doi: 10.1038/nature14299.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
61591pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNAA single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
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61592pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpAA human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).
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61593pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNAA single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
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61594pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpAA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.
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Antibodies from Article