A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
BMC Plant Biol. 2014 Nov 29;14(1):327. PubMed Article
Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
BMC Plant Biol. 2014 Nov 29;14(1):327. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50579 | pBUN6A11 | CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance |
| 50580 | pBUN6I11 | CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance |
| 50581 | pBUN411 | CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance |
| 50582 | pBUN501 | CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance |
| 50586 | pHSN6A01 | CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance |
| 50587 | pHSN6I01 | CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance |
| 50588 | pHSN401 | CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance |
| 50589 | pHSN501 | CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance |
| 50590 | pCBC-DT1T2 | CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites |
| 50591 | pCBC-DT2T3 | CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites |
| 50592 | pCBC-DT3T4 | CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites |
| 50593 | pCBC-MT1T2 | CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites |
| 50594 | pCBC-MT2T3 | CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites |
| 50595 | pCBC-MT3T4 | CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites |
| 62200 | pBUE411 | CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance |
| 62201 | pHSE401 | CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance |
| 62202 | pKSE401 | CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance |
| 62203 | pHUE411 | CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance |
| 62204 | pBUN421 | CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance |