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Independent optical excitation of distinct neural populations.
Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, Boyden ES
Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9. PubMed Article

Plasmids from Article

ID Plasmid Purpose
58805pAAV-CaMKII-Chronos-GFPAAV expression of Chronos-GFP under the CaMKII promoter
58806pAAV-Syn-ChrimsonR-GFPAAV expression of ChrimsonR-GFP under the Syn promoter
58841pAAV-Syn-CsChR-GFPAAV expression of CsChR-GFP under the Syn promoter
59049FCK-ChrimsonR-GFPLentiviral expression of ChrimsonR under the CaMKII promoter
59056pCAG-FLEX-rc[Chronos-GFP]Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)
59070pAAV-Syn-CoChR-GFPAAV-mediated expression of CoChR-GFP under the Syn promoter
59137pCAG-FLEX-fwd[Chrimson-tdT]Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)
59169pCAG-ChrimsonR-tdTExpression vector for ChrimsonR-tdTomato
59170pAAV-Syn-Chronos-GFPAAV-mediated expression of Chronos-GFP under the Syn promoter
59171pAAV-Syn-ChrimsonR-tdTAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter
62718pAAV-CaMKII-Chrimson-GFPAAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.
62722pAAV-Syn-FLEX-rc[Chronos-GFP]AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
62723pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.
62724pAAV-Syn-FLEX-rc[CoChR-GFP]AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
62725pAAV-EF1α-FLEX-rc[Chronos-GFP]AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
62726pAAV-Syn-Chronos-tdTomatoAAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.
80902FCK-Chronos-GFPLenti-mediated expression of Chronos-GFP under the CaMKII promoter.
84480pAAV-Syn-FLEX-rc [ChrimsonR-GFP]AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
84481pAAV-Syn-FLEX-rc [Chronos-tdTomato]AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.
84482pAAV-Syn-FLEX-fd [Chronos-GFP]AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.
84483pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.
84484pAAV-CAG-FLEX-rc [Chronos-tdTomato]AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.
84485pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
99231pAAV-CaMKII-ChrimsonR-tdTomatoAAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.
99232pAAV-CAG-Chronos-GFPAAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.
105834AAV-CAG-FLPX-rc [Chronos-tdTomato]AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.
108273pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
11154420XUAS IVS CsChrimson tdtomato_trOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato
1115455XUAS IVS CsChrimson tdtomato_trOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato
11154620XUAS IVS ChrimsonR mCherry_trOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry
11154710XUAS IVS ChrimsonR mCherry_trOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry
11154813XLexAop2 IVS ChrimsonR mCherry_trOptogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry
118295AAV-CAG-FLPX-rc [ChrimsonR-GFP]AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.
122063pAAV-EF1α1.1-ChrimsonR-GFPAAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.
122064pAAV-EF1α1.1-ChrimsonR-tdTomatoAAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
122098pAAV-EF1α1.1-Chronos-GFPAAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.
122099pAAV-EF1α1.1-Chronos-tdTomatoAAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
122100pAAV-EF1α1.1-GFPAAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.
122101pAAV-EF1α1.1-tdTomatoAAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
122102pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.
122103AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
128587AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
128588AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.
128589AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
130909AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

Antibodies from Article