CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den Heuvel S, Boxem M
Genetics. 2013 Aug 26. PubMed Article
Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den Heuvel S, Boxem M
Genetics. 2013 Aug 26. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47941 | pMB60 | Can be used to generate synthetic guide in vitro from T7 promotor |
| 47943 | pMB70 | Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences |
| 47944 | pMB62 | Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter |
| 47945 | pMB63 | Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter |
| 47946 | pMB66 | Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter |
| 47947 | pMB67 | Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter |