Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
Farzadfard F, Perli SD, Lu TK
ACS Synth Biol. 2013 Sep 11. PubMed Article
Farzadfard F, Perli SD, Lu TK
ACS Synth Biol. 2013 Sep 11. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 49013 | pTPGI_dCas9_VP64 | encodes yeast-optimized dCas9_VP64 synthetic transcription factor |
| 49014 | pRPR1_gRNA_handle_RPR1t | gRNA expression empty vector (yeast) |
| 49015 | pCMV_dCas9_VP64 | encodes human-optimized dCas9_VP64 synthetic transcription factor |
| 49016 | pU6_gRNA_handle_U6t | gRNA expression empty vector (mammalian cells) |
| 62966 | pRPR1(1xTetO)_gRNA_handle_RPR1t | aTc inducible gRNA expression vector |
| 64279 | pTPGI_dCas9 | encodes yeast-optimized dCas9 synthetic transcription factor |
| 64378 | pRPR1_a1gRNA_RPR1t | encodes a1 gRNA |
| 64379 | pRPR1_c1gRNA_RPR1t | encodes c1 gRNA |
| 64380 | pRPR1_c2gRNA_RPR1t | encodes c2 gRNA |
| 64381 | pRPR1_c3gRNA_RPR1t | encodes c3 gRNA |
| 64382 | pRPR1_c4gRNA_RPR1t | encodes c4 gRNA |
| 64383 | pRPR1_c5gRNA_RPR1t | encodes c5 gRNA |
| 64384 | pRPR1_c6gRNA_RPR1t | encodes c6 gRNA |
| 64385 | pRPR1_c7gRNA_RPR1t | encodes c7 gRNA |
| 64386 | pRPR1_c8gRNA_RPR1t | encodes c8 gRNA |
| 64387 | pRPR1_g1gRNA_RPR1t | encodes g1 gRNA |
| 64388 | pRPR1_g2gRNA_RPR1t | encodes g2 gRNA |
| 64389 | pCYC1m_yeGFP | encodes yeast enhanced GFP under control of minimal pCYC1 promoter |
| 64390 | 1x_a1gRNAOp_pCYC1m_yeBFP2 | encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP |
| 64391 | 3x_a1gRNAOp_pCYC1m_yeBFP2 | encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP |
| 64392 | 6x_a1gRNAOp_pCYC1m_yeBFP2 | encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP |
| 64393 | 12x_a1gRNAOp_pCYC1m_yeBFP2 | encodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP |