Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA
Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. PubMed Article
Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA
Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 44246 | pdCas9-humanized | Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown |
| 44247 | pdCas9::BFP-humanized | Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown |
| 44248 | pgRNA-humanized | Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown |
| 44249 | pdCas9-bacteria | aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown |
| 44250 | pwtCas9-bacteria | aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown |
| 44251 | pgRNA-bacteria | Expression of customizable guide RNA (gRNA) for bacterial gene knockdown |