A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis.
Bhabha G, Lee J, Ekiert DC, Gam J, Wilson IA, Dyson HJ, Benkovic SJ, Wright PE
Science. 2011 Apr 8;332(6026):234-8. doi: 10.1126/science.1198542. PubMed Article
Bhabha G, Lee J, Ekiert DC, Gam J, Wilson IA, Dyson HJ, Benkovic SJ, Wright PE
Science. 2011 Apr 8;332(6026):234-8. doi: 10.1126/science.1198542. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
110828 | pET27b-ecDHFR-N23PP | Bacterial expression of E.coli DHFR N23PP mutant |
110829 | pET27b-ecDHFR-N23PP/S148A | Bacterial expression of E.coli DHFR N23PP/S148A mutant |