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Site-specific integrase-mediated transgenesis in mice via pronuclear injection.
Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 10.1073/pnas.1019507108. Epub 2011 Apr 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
52491pBT317_pET-PhiC31o-pAfor preparation of PhiC31o mRNA
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52537pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning
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52538pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning
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52539pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning
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52540pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning
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52542pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning
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52543pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning
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52544pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus
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52545pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus
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52546pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus
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52547pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus
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52548pBT316_pattB-pCA-GFP-pAfor Phic31 integrase-mediated transgene integration
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52549pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pAtransgenic construct for expression of GFP and Flpo
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52550pBT344_pattB-pCA-GFP-pA-FRT5for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice
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52551pBT346_pLambda-attB-pCA-GFP-pAfor producing PhiC31 attB-containing minicircle in vitro
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52552pBT366_pattB-Hb9-GFP-pA-FRT5for PhiC31 integrase-mediated integration of pHb9-GFP
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52553pBT374_pattB-pCA-GFP-pA-attBSwafor PhiC31 integrase-mediated cassette exchange
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52554pBT378_pattB-pCA-GFP-pA-attBfor PhiC31 integrase-mediated cassette exchange
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Antibodies from Article