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A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos.
Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero C, Mueller F, Lemaire P
PLoS One. 2007. 2(9):e916. PubMed Article

Plasmids from Article

ID Plasmid Purpose
186350pDONR-221-P3-P5Gateway donor vector with AttP3 AttP5 recombination sites
186351pDONR-221-P3-P4Gateway donor vector with AttP3 AttP4 recombination sites
186355pENT-L1-Kozak-EGFP-GPI-stop-L2Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
186356pENT-L1-Kozak-GAP43-ECFP-stop-L2Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences
186357pENT-L1-Kozak-HistoneH2B-L2Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
186358pENT-L1-Kozak-NLSlacZ-stop-L2Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.
186359pENT-L1-Kozak-lacZ-stop-L2Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.
186360pENT-L1-Kozak-Venus-stop-L2Entry clone with ORF encoding Venus fluorescent protein flanked by Gateway recombination sequences
186361pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.
186362pENT-L1-Kozak-Ark-L2Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
186364pENT-L3-pFOGl-L5Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequences
186365pENT-L3-pSnail-L5Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequences
186366pENT-L3-a-element-L4Entry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequences
186367pENT-L3-Titf-L4Entry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequences
186368pENT-L3-eBra-L4Entry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequences
186369pENT-R4-bpFOG-L5Entry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequences
186370pDEST-Spe3-RfADestination vector for the expression of a protein of interest into embryos, by mRNA microinjection
186371pDEST-Spe3-Venus-RfADestination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjection
186372pDEST-Spe3-RfA-VenusDestination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjection
186373pDEST-Spe3-HA-RfADestination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjection
186374pDEST-Spe3-RfA-HADestination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjection
186396pDEST-pSP172BSSPE-Swa1::RfADestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.
186397pDEST-pSP172BSSPE-pFOGc::RfADestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.
186398pDEST-pSP172BSSPE-Swa1::venus-RfADestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.
186400pDEST-pSP172BSSPE-pFOGc::venus-RfADestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.
186401pDEST-pSP172BSSPE-pFOGc::RfA-venusDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.
186402pDEST-pSP172BSSPE-Swa1::RfA-ECFPDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.
186403PDEST-pSP172BSSPE-pFOGc::RfA-ECFPDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.
186404pDEST-pSP172BSSPE-Swa1::HA-RfADestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.
186405pDEST-pSP172BSSPE-Swa1::RfA-HADestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.
186406PDEST-pSP172BSSPE-pFOGc::RfA-HADestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.
186407pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfAMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.
186408pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfAMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.
186409pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-venusMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.
186410pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HAMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.
186411pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfAMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.
186412pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFPMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.
186413pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.
186414pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.

Antibodies from Article