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A noncanonical E-box enhancer drives mouse Period2 circadian oscillations in vivo.
Yoo SH, Ko CH, Lowrey PL, Buhr ED, Song EJ, Chang S, Yoo OJ, Yamazaki S, Lee C, Takahashi JS
Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2608-13. Epub 2005 Feb 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
48741pGL1Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to -141 with respect to transcription start site at +1)
48742pGL2Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)
48743pGL3Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)
48744pGL4Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1536 with respect to transcription start site at +1)
48745pGL5Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +2064 with respect to transcription start site at +1)
48746pGL6Expression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)
48747pGL3 basic E2Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)
48748pGL3 basic Mut E2Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)
48749pGL3 Pro E2Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)
48750pGL3 Pro Mut E2Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)

Antibodies from Article