Jonathan Arias Lab Materials

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• Plasmids
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Title	Authors	Publication
Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2).	Petruskeviciute A, Simuliunaite U, Polanco CM, Rojas B, Kuras S, Valatkaite-Rakstiene B, Norvilas R, Vijaya AK, Munoz P, Neniskyte U, Jakubauskas A, Burokas A, Nalvarte I, Inzunza J, Naumovas D, Stoskus M, Griskevicius L, Baltriukiene D, Arias J	Stem Cell Res. 2024 Dec 12;82:103628. doi: 10.1016/j.scr.2024.103628.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
	223321	pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9	SpCas9 codon optimized for human (cas9 Human, Synthetic), chloramphenicol resistance protein, CcdB	Mammalian Expression, CRISPR, Synthetic Biology	Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2). Stem Cell Res. 2024 Dec 12;82:103628. doi: 10.1016/j.scr.2024.103628.
	223323	pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9	SpCas9 and sgRNA-T2 (cas9 )	Mammalian Expression, CRISPR, Synthetic Biology	Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2). Stem Cell Res. 2024 Dec 12;82:103628. doi: 10.1016/j.scr.2024.103628.
	223328	pDONOR-MARINA	MARINA, KIR2.1 channel Golgi-to-plasma membrane trafficking signal	Mammalian Expression, CRISPR, Synthetic Biology	Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2). Stem Cell Res. 2024 Dec 12;82:103628. doi: 10.1016/j.scr.2024.103628.