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pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9
(Plasmid #223323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330_42230
  • Backbone manufacturer
    Feng Zhang, Addgene plasmid #42230
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 and sgRNA-T2
  • gRNA/shRNA sequence
    gggccactagggacaggat
  • Entrez Gene
    cas9 (a.k.a. SD90_RS03825, SD90_03830)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer U6_forward
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Expression vector from Cong et al. 10.1126/science.1231143 that carries the target sequence of Mali et al., 10.1126/science.1232033

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expression vector from Cong et al. 10.1126/science.1231143 that carries the target sequence of Mali et al., 10.1126/science.1232033

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9 was a gift from Jonathan Arias (Addgene plasmid # 223323 ; http://n2t.net/addgene:223323 ; RRID:Addgene_223323)

  • For your References section:

    Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2). Petruškevičiūtė A, Šimuliūnaitė U, Polanco CM, Rojas B, Kuras S, Valatkaitė-Rakštienė B, Norvilas R, Vijaya AK, Muñoz P, Neniškytė U, Jakubauskas A, Burokas A, Nalvarte I, Inzunza J, Naumovas D, Stoškus M, Griškevičius L, Baltriukienė D, Arias J. Stem Cell Res. 2024 Dec 12:103628. doi: 10.1016/j.scr.2024.103628. 10.1016/j.scr.2024.103628
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