Overcoming inefficient secretion of recombinant VEGF-C in baculovirus expression vector system by simple purification of the protein from cell lysate.
Klaus T, Kulesza M, Bzowska M, Wyroba B, Kilarski WW, Bereta J
Protein Expr Purif. 2015 Mar 7. pii: S1046-5928(15)00040-6. doi: 10.1016/j.pep.2015.03.001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64065 | gp64-mVEGF-C-HisTag-pENTR | Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system. |
| 64066 | HBM-mVEGF-C-HisTag-pENTR | Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system. |