A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish.
Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 10.1016/j.devcel.2015.01.032.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63154 | pME-Cas9 | Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS |
| 63155 | pME-Cas9-T2A-GFP | Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide. |
| 63156 | pDestTol2CG2-U6:gRNA | Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker. |
| 63157 | pDestTol2pA2-U6:gRNA | Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter. |