A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila.
Zhang X, Koolhaas WH, Schnorrer F
G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60944 | pJET1.2-STOP-dsRed | Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR. |
| 60945 | pBS-GGAC-TTCT | Drosophila, backbone for donor plasmid assembly. CRISPR knock-in. |
| 60948 | p3xP3-EGFP.vas-int.NLS | Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR. |
| 60949 | pBS-GGAC-ATGC | Drosophila, backbone for donor plasmid assembly. CRISPR knock-in. |
| 60950 | pBS-CGGA-GTGC | Drosophila, backbone for donor plasmid assembly. CRISPR knock-in. |
| 61255 | pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5 | CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette |
| 61256 | pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5 | CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette |
| 61257 | pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5 | CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette |