Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 57818 | pL-CRISPR.EFS.GFP | Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven |
| 57819 | pL-CRISPR.EFS.tRFP | Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven |
| 57821 | pLKO5d.EFS.SpCas9.P2A.BSD | Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven |
| 57822 | pLKO5.sgRNA.EFS.GFP | 3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven |
| 57823 | pLKO5.sgRNA.EFS.tRFP | 3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven |
| 57824 | pLKO5.sgRNA.EFS.tRFP657 | Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven |
| 57825 | pLKO5.sgRNA.EFS.PAC | Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven |
| 57826 | pL-CRISPR.SFFV.tRFP | Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven |
| 57827 | pL-CRISPR.SFFV.GFP | Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven |
| 57828 | pL-CRISPR.EFS.PAC | Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven |
| 57829 | pL-CRISPR.SFFV.PAC | Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven |
| 58329 | pLKO5d.EFS.SpCas9.P2A.PAC | Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven |
| 61395 | pL-SFFV.Reporter.RFP657.PAC | Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance. |