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High-sensitivity measurements of multiple kinase activities in live single cells.

Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
59138pENTR-ERKKTRCloverORF encoding ERK KTR mClover flanked by Gateway sequences
59139pENTR-JNKKTRCloverORF encoding JNK KTR mClover flanked by Gateway sequences
59142pENTR-p38KTRCloverORF encoding p38 KTR mClover flanked by Gateway sequences
59146pENTR-PKAKTRCloverORF encoding PKA KTR mClover flanked by gateway sequences
59148pENTR-JNKKTRmRuby2ORF encoding JNK KTR mRuby2 flanked by Gateway sequences
59149pENTR-p38KTRmCerulean3ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences
59150pLentiCMV Puro DEST ERKKTRCloverLentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)
59151pLentiPGK Puro DEST JNKKTRCloverLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)
59152pLentiPGK Puro DEST p38KTRCloverLentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)
59153pLentiPGK Puro DEST PKAKTRCloverLentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)
59154pLentiPGK Blast DEST JNKKTRmRuby2Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)
59155pLentiCMV Puro DEST p38KTRmCerulean3Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

Antibodies from Article