Independent optical excitation of distinct neural populations.
Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, Boyden ES
Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58805 | pAAV-CaMKII-Chronos-GFP | AAV expression of Chronos-GFP under the CaMKII promoter |
| 58806 | pAAV-Syn-ChrimsonR-GFP | AAV expression of ChrimsonR-GFP under the Syn promoter |
| 58841 | pAAV-Syn-CsChR-GFP | AAV expression of CsChR-GFP under the Syn promoter |
| 59049 | FCK-ChrimsonR-GFP | Lentiviral expression of ChrimsonR under the CaMKII promoter |
| 59056 | pCAG-FLEX-rc[Chronos-GFP] | Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on) |
| 59070 | pAAV-Syn-CoChR-GFP | AAV-mediated expression of CoChR-GFP under the Syn promoter |
| 59137 | pCAG-FLEX-fwd[Chrimson-tdT] | Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off) |
| 59169 | pCAG-ChrimsonR-tdT | Expression vector for ChrimsonR-tdTomato |
| 59170 | pAAV-Syn-Chronos-GFP | AAV-mediated expression of Chronos-GFP under the Syn promoter |
| 59171 | pAAV-Syn-ChrimsonR-tdT | AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter |
| 62718 | pAAV-CaMKII-Chrimson-GFP | AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal. |
| 62722 | pAAV-Syn-FLEX-rc[Chronos-GFP] | AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. |
| 62723 | pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] | AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version. |
| 62724 | pAAV-Syn-FLEX-rc[CoChR-GFP] | AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. |
| 62725 | pAAV-EF1α-FLEX-rc[Chronos-GFP] | AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. |
| 62726 | pAAV-Syn-Chronos-tdTomato | AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. |
| 80902 | FCK-Chronos-GFP | Lenti-mediated expression of Chronos-GFP under the CaMKII promoter. |
| 84480 | pAAV-Syn-FLEX-rc [ChrimsonR-GFP] | AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. |
| 84481 | pAAV-Syn-FLEX-rc [Chronos-tdTomato] | AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version. |
| 84482 | pAAV-Syn-FLEX-fd [Chronos-GFP] | AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. |
| 84483 | pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato] | AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version. |
| 84484 | pAAV-CAG-FLEX-rc [Chronos-tdTomato] | AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version. |
| 84485 | pAAV-EF1α1.1-FLEX-rc [Chronos-GFP] | AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. |
| 99231 | pAAV-CaMKII-ChrimsonR-tdTomato | AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal. |
| 99232 | pAAV-CAG-Chronos-GFP | AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal. |
| 105834 | AAV-CAG-FLPX-rc [Chronos-tdTomato] | AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. |
| 108273 | pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP] | AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. |
| 111544 | 20XUAS IVS CsChrimson tdtomato_tr | Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato |
| 111545 | 5XUAS IVS CsChrimson tdtomato_tr | Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato |
| 111546 | 20XUAS IVS ChrimsonR mCherry_tr | Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry |
| 111547 | 10XUAS IVS ChrimsonR mCherry_tr | Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry |
| 111548 | 13XLexAop2 IVS ChrimsonR mCherry_tr | Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry |
| 118295 | AAV-CAG-FLPX-rc [ChrimsonR-GFP] | AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner. |
| 122063 | pAAV-EF1α1.1-ChrimsonR-GFP | AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal. |
| 122064 | pAAV-EF1α1.1-ChrimsonR-tdTomato | AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal. |
| 122098 | pAAV-EF1α1.1-Chronos-GFP | AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal. |
| 122099 | pAAV-EF1α1.1-Chronos-tdTomato | AAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal. |
| 122100 | pAAV-EF1α1.1-GFP | AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal. |
| 122101 | pAAV-EF1α1.1-tdTomato | AAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal. |
| 122102 | pAAV-EF1α1.1-FLPX-rc [Chronos-GFP] | AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. |
| 122103 | AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato] | AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination. |
| 128587 | AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato] | AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination. |
| 128588 | AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP] | AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. |
| 128589 | AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] | AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination. |
| 130909 | AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] | AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination. |