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A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies.

Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 2014 Mar 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
58486pBabe JunD-HA neomammalian expression of mouse JunD
58489pBabe JUND-HA neomammalian expression of human JUND
58491pBabe RFP1-Smad2 neomammalian expression of mRFP1-tagged mouse Smad2
58493pBabe RFP1-KRT5 hygromammalian expression of mRFP1-tagged human KRT5
58496pBabe JunD-Venus puromammalian expression of Venus-tagged mouse JunD
58497pEN_TT Tgfbr3-HAGateway entry vector for an inducible HA-tagged mouse Tgfbr3
58498pEN_TT JunD-HAGateway entry vector for an inducible HA-tagged mouse JunD
58507pEN_TT S6K (E389-deltaCT)Gateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCT
58508pSLIK Tgfbr3-HA neoLentiviral expression vector for an inducible HA-tagged mouse Tgfbr3
58515pSLIK JunD-HA neoLentiviral expression vector for an inducible HA-tagged mouse JunD
58516pSLIK S6K (E389-deltaCT) neoLentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT
58518pTRF.1-mCMV-udsVenusEmpty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest
58692pTRF.1 udsVenus (P_JUND)A rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)
58696pLKO.1 shTGFBR3 puroLentiviral shRNA vector for knockdown of human TGFBR3
58698pLKO.1 shJUND puroLentiviral shRNA vector for knockdown of human JUND

Antibodies from Article