S-palmitoylation and ubiquitination differentially regulate interferon-induced transmembrane protein 3 (IFITM3)-mediated resistance to influenza virus.
Yount JS, Karssemeijer RA, Hang HC
J Biol Chem. 2012 Jun 1;287(23):19631-41. doi: 10.1074/jbc.M112.362095. Epub 2012 Apr 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58400 | pCMV-HA-mIFITM3-K24A | Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells |
| 58401 | pCMV-HA-mIFITM3-K83A | Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells |
| 58402 | pCMV-HA-mIFITM3-K88A | Expresses murine IFITM3-K88A with an N-terminal HA tag in mammalian cells |
| 58403 | pCMV-HA-mIFITM3-K104A | Expresses murine IFITM3-K104A with an N-terminal HA tag in mammalian cells |
| 58404 | pCMV-HA-mIFITM3-K24,83,88A | Expresses murine IFITM3-K24,83,88A with an N-terminal HA tag in mammalian cells |
| 58405 | pCMV-HA-mIFITM3-K83,88,104A | Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells |
| 58406 | pCMV-HA-mIFITM3-K24,88,104A | Expresses murine IFITM3-K24,88,104A with an N-terminal HA tag in mammalian cells |
| 58407 | pCMV-HA-mIFITM3-K24,83,104A | Expresses murine IFITM3-K24,83,104A with an N-terminal HA tag in mammalian cells |
| 58408 | pCMV-HA-mIFITM3-K24,83,88,104A | Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant) |
| 58410 | pCMV-HA-mIFITM3-N2A | Expresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site) |
| 58411 | pCMV-HA-mIFITM3-S38N | Expresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site) |
| 58412 | pCMV-HA-mIFITM3-D92N | Expresses murine IFITM3-D92N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site) |
| 58413 | pCMV-HA-mIFITM3-H136T | Expresses murine IFITM3-H136T with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site) |
| 58414 | pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL | Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells |