Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.
Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK
Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 53369 | pSQT1601 | human codon optimized Csy4 and FokI-dCas9 expression plasmid |
| 53370 | pSQT1313 | multiplex gRNA expression plasmid |
| 53371 | pSQT834 | Csy4 and Cas9 nuclease expression plasmid |
| 53372 | pNW3 | Csy4 and Cas9 D10A nickase expression plasmid |
| 53373 | pSQT817 | wildtype Cas9 expression plasmid |
| 60071 | RFN_EGFP_47 | Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease |
| 60072 | RFN_EGFP_80 | Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease |
| 60073 | RFN_EGFP_81 | Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease |
| 60074 | RFN_EGFP_82 | Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease |
| 60075 | RFN_EGFP_83 | Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease |
| 60076 | RFN_EGFP_84 | Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease |