Copper is required for oncogenic BRAF signalling and tumorigenesis.

Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S, Xiao K, Campbell SL, Thiele DJ, Counter CM
Nature. 2014 Apr 9. doi: 10.1038/nature13180. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
53202	pGEX4T3-HA-ERK2 D321N	Expresses GST and HA tagged human ERK2 D321N from bacterial expression vector
53201	pGEX4T3-HA-ERK2 R67S	Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector
53200	pGEX4T3-HA-ERK2 GOF	Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector
53180	pSUPER-retro-puro-tetO-CTR1-shRNA	Expresses shRNA against human CTR1 from puromycin resistance retroviral vector
53179	pSUPER-retro-puro-tetO-Mek1-shRNA	Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector
53177	pWZLblasti-HA-MEK1-MEK5DD	Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector
53175	pWZLblasti-HA-ERK5	Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector
53174	pWZLblasti-HA-ERK2 GOF	Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector
53173	pWZLblasti-HA-ERK2 D321N	Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector
53172	pWZLblasti-HA-ERK2 R67S	Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector