Copper is required for oncogenic BRAF signalling and tumorigenesis.
Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S, Xiao K, Campbell SL, Thiele DJ, Counter CM
Nature. 2014 Apr 9. doi: 10.1038/nature13180.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 53156 | pBABEbleo-Flag-BRAFV600E | Expresses Flag tagged human oncogenic BRAFV600E from bleomycin resistance retroviral vector |
| 53157 | pWZLblasti-CTR1 | Expresses human CTR1 from blasticidin resistance retroviral vector |
| 53158 | pWZLblasti-CTR1M154A | Expresses human CTR1 M154A from blasticidin resistance retroviral vector |
| 53159 | pGEX6P1-HA-MEK1 | Expresses GST-HA tagged human MEK1 from bacterial expression vector |
| 53161 | pWZLblasti-HA-MEK1 | Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector |
| 53163 | pGEX6P1-HA-MEK1 CBM | Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector |
| 53164 | pGEX6P1-HA-MEK1 C121S | Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector |
| 53165 | pWZLblasti-HA-MEK1 C121S | Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector |
| 53166 | pGEX6P1-HA-MEK5 | Expresses GST-HA tagged human MEK5 from bacterial expression vector |
| 53167 | pWZLblasti-HA-MEK5 | Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector |
| 53168 | pGEX6P1-HA-MEK5 CBM-E | Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector |
| 53169 | pWZLblasti-HA-MEK5 CBM-E | Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector |
| 53170 | pGEX4T3-HA-ERK2 K54R | Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector |
| 53171 | pGEX6P1-HA-ERK5 K84R | Expresses GST-HA tagged human ERK5 K84R from bacterial expression vector |
| 53172 | pWZLblasti-HA-ERK2 R67S | Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector |
| 53173 | pWZLblasti-HA-ERK2 D321N | Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector |
| 53174 | pWZLblasti-HA-ERK2 GOF | Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector |
| 53175 | pWZLblasti-HA-ERK5 | Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector |
| 53176 | pBABEpuro-HA-MEK1-MEK5DD | Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from puromycin resistance retroviral vector |
| 53177 | pWZLblasti-HA-MEK1-MEK5DD | Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector |
| 53178 | pBABEpuro-CMYC T58A | Expresses mouse CMYC T58A from puromycin resistance retroviral vector |
| 53179 | pSUPER-retro-puro-tetO-Mek1-shRNA | Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector |
| 53180 | pSUPER-retro-puro-tetO-CTR1-shRNA | Expresses shRNA against human CTR1 from puromycin resistance retroviral vector |
| 53195 | pBABEpuro-HA-MEK1 | Expresses HA tagged human MEK1 from puromycin resistance retroviral vector |
| 53196 | pBABEpuro-HA-MEK1 CBM | Expresses HA tagged human MEK1 M187A/H188A/M230A/H239A from puromycin resistance retroviral vector |
| 53197 | pBABEpuro-HA-MEK1 C121S | Expresses HA tagged human MEK1 C121S from puromycin resistance retroviral vector |
| 53198 | pBABEpuro-HA-MEK5 | Expresses HA tagged human MEK5 from puromycin resistance retroviral vector |
| 53199 | pBABEpuro-HA-MEK5 CBM-E | Expresses HA tagged human MEK5 L280A/H281A/M323A/Q332A from puromycin resistance retroviral vector |
| 53200 | pGEX4T3-HA-ERK2 GOF | Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector |
| 53201 | pGEX4T3-HA-ERK2 R67S | Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector |
| 53202 | pGEX4T3-HA-ERK2 D321N | Expresses GST and HA tagged human ERK2 D321N from bacterial expression vector |
| 53203 | pBABEpuro-HA-ERK2 GOF | Expresses HA tagged human ERK2 R67S D321N from puromycin resistance retroviral vector |