Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC
Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51129 | pST1374-N-NLS-flag-linker-Cas9-H840A | Cas9 nickase optimized for nuclear import. |
| 51130 | pST1374-N-NLS-flag-linker-Cas9-D10A | Cas9 nickase optimized for nuclear import. |
| 51132 | pUC57-sgRNA expression vector | For in vitro transcription of sgRNA from the T7 promoter. |
| 51133 | pGL3-U6-sgRNA-PGK-puromycin | expresses sgRNA from the U6 promoter. |