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Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs.

Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A, Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein MB, Snyder M, Turk BE
Sci Signal. 2010 Feb 16;3(109):ra12. doi: 10.1126/scisignal.2000482. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
52681pGEX4T2-Ste7-EEexpression of constitutively active Ste7 kinase in bacteria
52682pET28-Kss1Expression of Kss1 in bacteria

Antibodies from Article