Site-specific integrase-mediated transgenesis in mice via pronuclear injection.
Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 10.1073/pnas.1019507108. Epub 2011 Apr 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52491 | pBT317_pET-PhiC31o-pA | for preparation of PhiC31o mRNA |
| 52537 | pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5 | Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning |
| 52538 | pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5 | Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning |
| 52539 | pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5 | Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning |
| 52540 | pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5 | Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning |
| 52542 | pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5 | Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning |
| 52543 | pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5 | Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning |
| 52544 | pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5 | Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus |
| 52545 | pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5 | Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus |
| 52546 | pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5 | Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus |
| 52547 | pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5 | Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus |
| 52548 | pBT316_pattB-pCA-GFP-pA | for Phic31 integrase-mediated transgene integration |
| 52549 | pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA | transgenic construct for expression of GFP and Flpo |
| 52550 | pBT344_pattB-pCA-GFP-pA-FRT5 | for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice |
| 52551 | pBT346_pLambda-attB-pCA-GFP-pA | for producing PhiC31 attB-containing minicircle in vitro |
| 52552 | pBT366_pattB-Hb9-GFP-pA-FRT5 | for PhiC31 integrase-mediated integration of pHb9-GFP |
| 52553 | pBT374_pattB-pCA-GFP-pA-attBSwa | for PhiC31 integrase-mediated cassette exchange |
| 52554 | pBT378_pattB-pCA-GFP-pA-attB | for PhiC31 integrase-mediated cassette exchange |