Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system.
Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V
Plant Methods. 2013 Oct 11;9(1):39.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
49770 | pICH41308::hCas9 | Level 0 hCas9 module |
49771 | pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST | Level 1 hCas9 module |
49772 | pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1 | Level 2 construct |