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Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system.

Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V
Plant Methods. 2013 Oct 11;9(1):39. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
49770pICH41308::hCas9Level 0 hCas9 module
49771pICH47742::2x35S-5’UTR-hCas9(STOP)-NOSTLevel 1 hCas9 module
49772pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1Level 2 construct

Antibodies from Article