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Addgene

pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST
(Plasmid #49771)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49771 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pICH47742
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    35Sp::hCas9
  • Species
    Synthetic

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    hCas9 from George Church
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We removed BsaI and BbsI sites from hCas9 preserving the amino acid composition and placed it under the 35S promoter using the Golden Gate system from S. Marillonnet.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST was a gift from Sophien Kamoun (Addgene plasmid # 49771 ; http://n2t.net/addgene:49771 ; RRID:Addgene_49771)
  • For your References section:

    Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V. Plant Methods. 2013 Oct 11;9(1):39. 10.1186/1746-4811-9-39 PubMed 24112467