Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
Farzadfard F, Perli SD, Lu TK
ACS Synth Biol. 2013 Sep 11.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 49013 | pTPGI_dCas9_VP64 | encodes yeast-optimized dCas9_VP64 synthetic transcription factor |
| 49014 | pRPR1_gRNA_handle_RPR1t | gRNA expression empty vector (yeast) |
| 49015 | pCMV_dCas9_VP64 | encodes human-optimized dCas9_VP64 synthetic transcription factor |
| 49016 | pU6_gRNA_handle_U6t | gRNA expression empty vector (mammalian cells) |
| 62966 | pRPR1(1xTetO)_gRNA_handle_RPR1t | aTc inducible gRNA expression vector |
| 64279 | pTPGI_dCas9 | encodes yeast-optimized dCas9 synthetic transcription factor |
| 64378 | pRPR1_a1gRNA_RPR1t | encodes a1 gRNA |
| 64379 | pRPR1_c1gRNA_RPR1t | encodes c1 gRNA |
| 64380 | pRPR1_c2gRNA_RPR1t | encodes c2 gRNA |
| 64381 | pRPR1_c3gRNA_RPR1t | encodes c3 gRNA |
| 64382 | pRPR1_c4gRNA_RPR1t | encodes c4 gRNA |
| 64383 | pRPR1_c5gRNA_RPR1t | encodes c5 gRNA |
| 64384 | pRPR1_c6gRNA_RPR1t | encodes c6 gRNA |
| 64385 | pRPR1_c7gRNA_RPR1t | encodes c7 gRNA |
| 64386 | pRPR1_c8gRNA_RPR1t | encodes c8 gRNA |
| 64387 | pRPR1_g1gRNA_RPR1t | encodes g1 gRNA |
| 64388 | pRPR1_g2gRNA_RPR1t | encodes g2 gRNA |
| 64389 | pCYC1m_yeGFP | encodes yeast enhanced GFP under control of minimal pCYC1 promoter |
| 64390 | 1x_a1gRNAOp_pCYC1m_yeBFP2 | encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP |
| 64391 | 3x_a1gRNAOp_pCYC1m_yeBFP2 | encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP |
| 64392 | 6x_a1gRNAOp_pCYC1m_yeBFP2 | encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP |
| 64393 | 12x_a1gRNAOp_pCYC1m_yeBFP2 | encodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP |