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Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination.

Chen C, Fenk LA, de Bono M
Nucleic Acids Res. 2013 Sep 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
48960peft-3::cas-9::tbb-2 3'UTRthis plasmid contains the codon optimized gene encoding Cas9 for C. elegans.
48961sgRNA with rpr-1 promoterto drive the sgRNA expression under RNase P non-coding RNA promoter
48962sgRNA with U6 promoterto drive the sgRNA expression under a U6 promoter

Antibodies from Article