Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination.
Chen C, Fenk LA, de Bono M
Nucleic Acids Res. 2013 Sep 5.
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PubMed
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
48960 | peft-3::cas-9::tbb-2 3'UTR | this plasmid contains the codon optimized gene encoding Cas9 for C. elegans. |
48961 | sgRNA with rpr-1 promoter | to drive the sgRNA expression under RNase P non-coding RNA promoter |
48962 | sgRNA with U6 promoter | to drive the sgRNA expression under a U6 promoter |