sgRNA with rpr-1 promoter
(Plasmid
#48961)
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Purpose(Empty Backbone) to drive the sgRNA expression under RNase P non-coding RNA promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMA-T
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Backbone manufacturerlife technologies
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Vector typeWorm Expression, CRISPR
- Promoter rpr-1 promoter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer acaaaaaggctcagcctcaacc
- 3′ sequencing primer tttgaaattttgagtgaggctcagag (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sgRNA with rpr-1 promoter was a gift from Mario de Bono (Addgene plasmid # 48961 ; http://n2t.net/addgene:48961 ; RRID:Addgene_48961) -
For your References section:
Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination. Chen C, Fenk LA, de Bono M. Nucleic Acids Res. 2013 Sep 5. 10.1093/nar/gkt805 PubMed 24013562