Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.
Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R
Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48214 | pAC1-pCR8-dCas9VP48 | dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression |
| 48218 | pAC84-pCR8-dCas9 | dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression |
| 48219 | pAC147-pCR8-dCas9VP64 | dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression |
| 48220 | pAC148-pCR8-dCas9VP96 | dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression |
| 48221 | pAC149-pCR8-dCas9VP160 | dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression |
| 48222 | pAC90-pmax-DEST | Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector |
| 48223 | pAC91-pmax-dCas9VP64 | dCas9VP64 on pmax expression vector |
| 48224 | pAC92-pmax-dCas9VP96 | dCas9VP96 on pmax expression vector |
| 48225 | pAC93-pmax-dCas9VP160 | dCas9VP160 on pmax expression vector |
| 48226 | pAC94-pmax-dCas9VP160-2A-puro | dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested. |
| 48227 | pAC95-pmax-dCas9VP160-2A-neo | dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested. |
| 48228 | pAC103-PBNeo-U6-sgRNA-Expression | sgRNA expression on PiggyBac vector with Neo-selectable marker |
| 48234 | pAC150-PBLHL-4xHS-EF1a-DEST | PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker |
| 48236 | pAC2-dual-dCas9VP48-sgExpression | Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters |
| 48237 | pAC5-dual-dCas9VP48-sgTetO | Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters |
| 48238 | pAC152-dual-dCas9VP64-sgExpression | Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters |
| 48239 | pAC153-dual-dCas9VP96-sgExpression | Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters |
| 48240 | pAC154-dual-dCas9VP160-sgExpression | Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters |
| 49045 | pAC155-pCR8-sgExpression | U6-driven sgRNA expressing cassette on a gateway donor vector |